Abstract: The invention related to hyaluronic derivative according to formula (I), methods of preparation thereof and a hydrogel prepared obtained from the derivative and methods of preparation thereof. The hydrogel can be used in tissue engineering, cosmetics, medicine or regenerative medicine such as the forming of scaffolds for the treatment of articular cartilage or bone tissue defects.
Type:
Grant
Filed:
February 26, 2013
Date of Patent:
November 15, 2016
Assignee:
Contipro Biotech s.r.o.
Inventors:
Lucie Wolfova, Martin Pravda, Marcela Foglarova, Miroslava Nemcova, Krzysztof Niedoba, Vladimir Velebny
Abstract: The present invention relates to the detection of tumor stem cells and tumor cells in epithelial-mesenchymal transition and uses of such methods. According to the present invention said method comprises a selecting step for selection or enrichment of said predetermined cells from the sample wherein the sample is contacted with the solid surface for preferential binding of said predetermined cells to the solid surface and then the sample is removed d from the solid surface in a washing step. The inventive method is characterized in that the sample contains a polyol at least during one of contacting the sample with the solid surface and the washing step and in a detection step detecting in said cells, preferentially selected or enriched by said selecting step, the presence or absence of expression of at least one marker associate with at least one the group comprising tumor stem cells and tumor cells in epithelial-mesenchymal transition.
Abstract: Methods of treating an arthritic joint of a subject, including administering a pharmaceutical composition by injection into the arthritic joint, wherein the composition includes an anthocyanin or anthocyanidin, glucose, and a pharmaceutically acceptable carrier.
Abstract: Disclosed herein are isotopically labeled antifungal antibiotics and related compounds. Also disclosed are methods for synthesizing these isotopically labeled molecules and using the same to study the distribution of these compounds in the biosphere as well as the products formed by the breakdown of these isotopically labeled compounds.
Type:
Grant
Filed:
December 23, 2013
Date of Patent:
November 1, 2016
Inventors:
Ronald Ross, Jr., Peter Lee Johnson, Kevin G. Meyer
Abstract: The invention relates to a culture substrate having thermoresponsive microgel particles that have a thermoresponsive polymer and modulator particles fixed to the carrier area of the substrate. The modulator can be a substance that has an adhesion capability with biological cells or a substance that causes cellular reactions that are inducible to binding to surface receptors of biological cells. Methods for preparation and use in culturing cells are given.
Type:
Grant
Filed:
March 21, 2011
Date of Patent:
October 25, 2016
Assignee:
Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
Inventors:
Claus Duschl, Andreas Lankenau, Stephan Schmidt, Thomas Hellweg, Erik Wischerhoff, Andre Laschewsky, Jean-Francois Lutz
Abstract: A method for separating cells which includes: adhering cells to the surface of a cell culture substrate containing a photo-acid generator that generates an acidic substance upon irradiation with active energy rays, and irradiating only a partial region of the cell culture substrate with the active energy rays to selectively remove the cells within the partial region, thereby separating the cells within the partial region and cells in other regions.
Type:
Grant
Filed:
March 28, 2011
Date of Patent:
September 6, 2016
Assignee:
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
Abstract: The invention relates to a method for the production of adenosine 3?,5?-monophosphate (cAMP) by a permeable microbial strain which uses polyols for protecting activities of enzymes and a cAMP precursor and phosphate as substrates. Glucose is used as an energy provider and metal ions and an organic solvent such as acetone are used in the medium.
Abstract: Disclosed are molecules and their synthesis. The fluorescent moiety also facilitates screening, tracking, and pharmacodynamic studies of the drug in a biological system both in vitro and in vivo.
Type:
Grant
Filed:
August 17, 2009
Date of Patent:
August 9, 2016
Assignee:
Georgetown University
Inventors:
Venkata Mahidhar Yenugonda, Milton L. Brown
Abstract: Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregation-induced emission (AIE) properties, which can be used for long-term cell tracking. The luminogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. The fluorescent molecules can readily pass through cell membranes, stain only the cell cytoplasm, and form highly emissive nanoaggregates in aqueous media without any obvious cytoxicity in the living cells. Furthermore, the molecules can be retained inside the cells without noticeable leakage to the outside. Therefore, these AIE-based compounds can be used as selective and cell-compatible fluroescent bioprobes for long-term live cell tracking and imaging.
Type:
Grant
Filed:
October 11, 2012
Date of Patent:
August 9, 2016
Assignee:
THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventors:
Benzhong Tang, Ka Ming Ng, Qian Luo, Yong Yu, Yuning Hong, Jianzhao Liu, Sijie Chen, Wing Yip Lam
Abstract: A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.
Type:
Grant
Filed:
August 6, 2012
Date of Patent:
July 12, 2016
Assignees:
Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
Abstract: Compositions and methods are disclosed for substantially liquid, gel, suspension, slurry, semisolid and/or colloid storage of biological samples following admixture with the herein disclosed storage composition, permitting substantial recovery of biological activity following storage without refrigeration. In certain embodiments, unfractionated blood or tissue samples may be stored without refrigeration in a form that permits recovery of intact DNA or RNA components following the storage period.
Type:
Grant
Filed:
July 26, 2011
Date of Patent:
June 28, 2016
Assignee:
Biomatrica, Inc.
Inventors:
Scott E. Whitney, Steven Wilkinson, Rolf Muller
Abstract: A fluorophore and methods of detecting cations and hydrophobic environments using the fluorophore are disclosed. The fluorophore includes an imidazo[1,5-a]pyridinium ion core and has the formula where R1, R2, R3 and X? are as defined in the specification, and n is an integer from 1 to 4.
Type:
Grant
Filed:
March 4, 2013
Date of Patent:
June 21, 2016
Assignee:
INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION
Inventors:
Johnathon T. Hutt, Zachary David Aron, Dongwhan Lee, Junyong Jo
Abstract: The invention relates to novel caged ceramide 1-phosphate (C1P), caged ceramide 1-phosphate derivatives(C1P), and the method of using them for delivering C1P intracellularly in vitro and in vivo, for research and therapeutic purposes. Therapeutic purposes include treatment of diseases associated with cell death, such as neurodegerative diseases, including Alzheimer's disease. The disclosed compositions may also be used for wound healing.
Type:
Grant
Filed:
October 26, 2010
Date of Patent:
June 7, 2016
Assignee:
RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK
Abstract: Disclosed is a compound which is a two-photon absorbing fluorescent substance, a production method for the compound, a fluorescence sensor and molecular probe able to sense various substrates or enzyme activity or the like using the same, and a method of sensing enzyme activity or the like using the same. The disclosed compound can be used as a fluorescence sensor, and can be used in the study and treatment of diseases in which MAO enzymes are involved such as mood disorders using MAO enzyme activity and inhibitor screening.
Abstract: The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
May 24, 2016
Assignee:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
Type:
Grant
Filed:
June 1, 2012
Date of Patent:
May 24, 2016
Assignee:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: Generally, compositions and methods for handling processed sperm populations including samples that are freshly collected, transported as fresh samples, as well as samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the sperm cell. Such trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the sperm's ability to fertilize an egg, grow into a healthy embryo and produce a healthy offspring. The novel compounds described can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
May 24, 2016
Assignee:
Inguran, LLC
Inventors:
Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
Abstract: A container for forming a cell aggregate is provided in which a group represented by a general formula of: (in the formula, each of R1, R2, and R3 is independently a an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less.) and at least one of an amino group, a carboxyl group, and a hydroxyl group are present near a surface thereof.
Type:
Grant
Filed:
June 15, 2010
Date of Patent:
May 24, 2016
Assignee:
SHISEIDO COMPANY, LTD.
Inventors:
Shigeyoshi Fujiwara, Keigo Takei, Aya Hirayama, Yukimitsu Suda
Abstract: The invention related to the use of Bacillus licheniformis strain OBT618 (ATCC PTA-122188) for inhibiting Clostridium pathogens in a material contaminated with the pathogens. The strain can be added to animal feed, animal waste or food processing facility waste that is contained with Clostridium to inhibit the pathogen.
Type:
Grant
Filed:
March 14, 2014
Date of Patent:
April 12, 2016
Assignee:
Osprey Biotechnics, Inc.
Inventors:
Christopher J. Reuter, Steven J. MacKenzie, Lauren G. Danielson, Vincent Scuilla