Patents Examined by Susan M. Hanley
  • Patent number: 10842173
    Abstract: Disclosed are compositions and methods for producing a poultry egg that is rich in odd-chain fatty acid (OCFA), particularly pentadecanoic acid (C15:0) and heptadecanoic (C17:0) acid. Poultry feed can be mixed with a compound (e.g. biomass or oil extract) that is produced from microalgae cultured to comprise elevated levels of OCFA. The OCFA-enriched poultry feed comprises an elevated level of OCFA and it can be fed to poultry that are laying eggs. As the OCFA-enriched feed is incorporated by the poultry, the resulting eggs comprise yolks that are rich in OCFA. The OCFA rich eggs can be consumed by humans as a dietary source of OCFA, to improve health.
    Type: Grant
    Filed: September 20, 2019
    Date of Patent: November 24, 2020
    Assignee: Heliae Development, LLC
    Inventors: Jamie Evans, Micheal LaMont
  • Patent number: 10836767
    Abstract: Described are pro-coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays or fragment complementary luciferase. The disclosed pro-coelenterazine analogues provide increased serum stability for live cell assays, and are capable of tuning the brightness and assay windows as needed for the applications.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: November 17, 2020
    Inventors: Wenhui Zhou, Brock Binkowski, Poncho Meisenheimer, Andrew L. Niles, Kevin Kupcho, James Unch
  • Patent number: 10829799
    Abstract: The present invention relates to the production of activated biological molecules for use in profiling biological molecule interactions. The activated biological molecule may be a ubiquitin (Ub) and ubiquitin-like conjugation enzyme as well as other proteins with internal reactive cysteine residues.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: November 10, 2020
    Inventors: Satpal Virdee, Mathew Stanley, Kuan-Chuan Pao
  • Patent number: 10830763
    Abstract: Provided is a component analysis device, which comprises an analysis unit for measuring a component fed to the plurality of containers and analyzing the component thus measured, wherein the plurality of containers are at least a first container and a second container, wherein the first container retains a first solution containing a substance that releases adhesion between multiple cells forming the liver cell tissue, and the second container retains a buffer solution, and wherein the analysis unit measures an amount of the component discharged from the liver cell tissue in the first container into the first solution and an amount of the component discharged from the liver cell tissue in the second container into the buffer solution in the second container, and analyzes an amount of the component to be discharged via a bile duct in the liver cell tissue.
    Type: Grant
    Filed: January 5, 2016
    Date of Patent: November 10, 2020
    Inventors: Ryosuke Takahashi, Katsuhiro Kanda, Yusuke Shimizu
  • Patent number: 10827767
    Abstract: The present invention relates to the use of turbine mixing during enzymatic hydrolysis of aquatic protein from species such as fish, aquatic mammals, crustaceans and/or mollusks, to obtain high quality aquatic protein hydrolysates, having very low oxidation, improved organoleptic profile and improved biological activity of interest, for human consumption and cosmetics. The turbine mixing can inhibit oxidation during hydrolysis, contribute to an increase in the bio-activity and decrease the bitter taste of the final product. The process can vary in starting material, pre-treatment, type and amount of enzyme, hydrolysis conditions, time, degree of hydrolysis and post-treatment.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: November 10, 2020
    Inventors: Bomi Framroze, Roald Rogne
  • Patent number: 10815379
    Abstract: Fluorescent probes and their salts have high fluorescence quantum yield and high resistance to photobleaching suitable for visualizing various pH environments within cells such as weakly basic, neutral, and weakly acidic environments.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: October 27, 2020
    Inventors: Kenjiro Hanaoka, Yu Kagami, Tetsuo Nagano, Yasuteru Urano
  • Patent number: 10815247
    Abstract: Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.
    Type: Grant
    Filed: December 28, 2017
    Date of Patent: October 27, 2020
    Inventors: Roderick G. Flemming, Mary Hall, Thomas Machleidt, Poncho Meisenheimer, Nidhi Nath, Matthew B. Robers, Joel R. Walker, Wenhui Zhou
  • Patent number: 10793501
    Abstract: The invention pertains to a method for providing a succinic acid solution, comprising the steps of —providing a first magnesium succinate containing medium with a magnesium succinate concentration of 18-23 wt. % to a first acidification reactor where it is contacted with hydrogen chloride to form a solution of succinic acid, magnesium chloride and hydrogen chloride, —providing a second magnesium succinate containing medium with a magnesium succinate concentration of 25-50 wt. %, and contacting it in a second acidification reactor with the solution of succinic acid, magnesium chloride and hydrogen chloride withdrawn from the first acidification reactor, to form an aqueous mixture comprising magnesium chloride and succinic acid with a succinic acid concentration of at least 18 wt. %. The method according to the invention makes it possible to obtain a solution comprising succinic acid and magnesium 20 chloride with an increased succinic acid concentration.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: October 6, 2020
    Assignee: PURAC BIOCHEM B.V.
    Inventors: Peter Paul Jansen, Jan Van Breugel, Gerard Hendrik Van Bochove, Jose Maria Vidal Lancis, Tanja Dekic Zivkovic
  • Patent number: 10774234
    Abstract: The present invention is to provide a cell culture vessel comprising a copolymer which contains a recurring unit containing an organic group of the following formula (a) and a recurring unit containing an organic group of the following formula (b) being coated onto a surface thereof, a method for manufacturing the same and a method for manufacturing a cell aggregate using the same (wherein Ua1, Ua2, Ub1, Ub2 and Ub3, and An? are as defined in the present specification and the claims).
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: September 15, 2020
    Inventors: Ayako Otani, Taito Nishino, Yoshiomi Hiroi, Takahiro Kishioka, Tomoyuki Ozawa
  • Patent number: 10774394
    Abstract: The disclosure provides a system for production of reactive intermediates from lignocellulosic biomass. The reactive intermediates can be used as platform chemicals for biological conversions or can be further catalytically upgraded to be used as “drop in” reagents for fuels. The disclosure provides methods and compositions useful for processing biomass to biofuels and intermediates.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: September 15, 2020
    Assignee: The Regents of the University of California
    Inventors: Charles M. Cai, Charles E. Wyman, Taiying Zhang, Rajeev Kumar
  • Patent number: 10767141
    Abstract: A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: September 8, 2020
    Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
    Inventors: Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Patent number: 10751367
    Abstract: An aluminoborate composition, an alumino-borosilicate glass composition, or a mixture thereof, and solid or hollow microspheres thereof, as defined herein. Also disclosed are methods of making and using the disclosed compositions, for example, forming microspheres for use in bioactive applications, and composition extracts for use in treating or healing wounds.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: August 25, 2020
    Assignee: Corning Incorporated
    Inventors: Huayun Deng, Ye Fang, Qiang Fu, Lina Ma, John Christopher Mauro, Michael Joshua Snyder, Dana Lynne Widmer-Quiel
  • Patent number: 10729776
    Abstract: A composition includes a plurality of coacervate micro and/or nanodroplets of oxidized alginate and a methacrylated gelatin.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: August 4, 2020
    Inventors: Eben Alsberg, Oju Jeon, David Wolfson
  • Patent number: 10730892
    Abstract: Probes for formaldehyde (FA) including a homoallylamine trigger group attached to a detectable moiety are provided. Aspects of the probes include luminogenic or fluorogenic probes, such as a probe including a quencher in energy-receiving proximity to a fluorophore. Also provided are methods of using the probes for sensitive and bio orthogonal detection of FA in a sample. Aspects of the methods include selectively reacting the probe with the formaldehyde in the sample to a release (e.g., via a 2-aza-Cope rearrangement) a reporter group comprising a detectable moiety. Aspects of the methods detecting formaldehyde in a cell, tissue, organ or fluid in a subject. Also provided are compositions and kits including the subject probes that find use in practicing various embodiments of the subject methods.
    Type: Grant
    Filed: August 18, 2016
    Date of Patent: August 4, 2020
    Assignee: The Regents of the University of California
    Inventors: Christopher J. Chang, Thomas Francis Brewer, Jefferson Chan
  • Patent number: 10732172
    Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.
    Type: Grant
    Filed: May 22, 2016
    Date of Patent: August 4, 2020
    Assignees: President and Fellows of Harvard College, Children's Medical Center Corporation
    Inventors: Donald E. Ingber, Abhishek Jain, Andries D. van der Meer, Alan David Michelson, Andrew L. Frelinger, III, Riccardo Barrile
  • Patent number: 10731182
    Abstract: The use of a product for internal dehydration of hydrogenated sugar as a methanogen substrate in a method for biogas production, a composition including a monoanhydrohexitol (M), a dianhydrohexitol (D), and anhydrohexitol polymers (P), and a methanisation method.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: August 4, 2020
    Inventors: Herve Wyart, Mathias Ibert
  • Patent number: 10725055
    Abstract: Disclosed herein are embodiments of donor compounds that can be used to produce H2S from COS or CS2 released from the donor compounds. In some embodiments, the donor compounds can indirectly produce H2S after being exposed to a reactive component in a triggering event. In other embodiments, the donor compounds can indirectly regenerate H2S after reacting with an H2S analyte. The donor compounds disclosed herein can be used for analytical techniques, disease diagnostics, and/or therapeutic applications. Methods of making and using the donor compounds also are provided herein.
    Type: Grant
    Filed: April 10, 2017
    Date of Patent: July 28, 2020
    Assignee: University of Oregon
    Inventors: Michael D. Pluth, Andrea Steiger, Yu Zhao
  • Patent number: 10718694
    Abstract: The present disclosure relates to counterstains for staining a biological sample on a single slide in preparation for microscopic examination. The counterstains are used to analyze the sample on the single slide using both brightfield and fluorescent illumination. The counterstains can identify both morphological details and molecular structures within the cells contained in the sample. The counterstains can be used in conjunction with other molecular stains.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: July 21, 2020
    Assignees: Diagnostic BioSystems
    Inventor: Marc Key
  • Patent number: 10718006
    Abstract: An electrochemical method is provided for in-vitro determination of the presence of bacteria producing lactamases, in a sample that may contain such bacteria.
    Type: Grant
    Filed: April 1, 2016
    Date of Patent: July 21, 2020
    Inventors: Murielle Dequaire-Rochelet, Alain Hartmann, Catherine Neuwirth, Benoit Chantemesse
  • Patent number: 10718005
    Abstract: Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: July 21, 2020
    Assignee: Enzo Life Sciences, Inc
    Inventors: Irina Lebedeva, Wayne Forrest Patton, Maciej Szczepanik, Yuejun Xiang, Praveen Pande