Abstract: High aldehyde dehydrogenase 1A1 (ALDH1A1) activity has emerged as a reliable marker for the identification of both normal and cancer stem cells. Herein, is presented AlDeSense, a turn-on green fluorescent probe for aldehyde dehydrogenase 1A1 (ALDH1A1) and Ctrl-AlDeSense, a matching non-responsive reagent. AlDeSense exhibits a 20-fold fluorescent enhancement when treated with ALDH1A1. Through the application of surface marker antibody staining, tumorsphere assays, and assessment of tumorigenicity, the disclosed results show that cells exhibiting high AlDeSense signal intensity have properties of cancer stem cells. Herein, is also reported the development of a red congener, red-AlDeSense. Importantly, red-AlDeSense represents one of only a few examples of a turn-on sensor in the red region using the d-PeT quenching mechanism.
Type:
Grant
Filed:
December 18, 2019
Date of Patent:
August 9, 2022
Assignee:
The Board of Trustees of the University of Illinois
Inventors:
Thomas Edward Bearrood, Chelsea Diane Anorma, Jefferson Chan
Abstract: Disclosed herein are compositions related to conjugated polypeptides with MTA/ADO-degrading enzyme activity. The conjugated polypeptides are engineered to allow for maximal conjugation while maintaining catalytic activities. Also disclosed are nucleic acids, expression vectors, and host cells related to the conjugated polypeptides. Further disclosed are methods of using the pharmaceutical formulations comprising above to treat cancer.
Type:
Grant
Filed:
January 6, 2021
Date of Patent:
July 26, 2022
Assignee:
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Abstract: Provided herein are methods of predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor, methods of improving cell viability in a production bioreactor, methods of predicting cell viability in a production bioreactor, and methods for culturing a cell in a production bioreactor.
Type:
Grant
Filed:
March 15, 2019
Date of Patent:
July 19, 2022
Assignee:
Genzyme Corporation
Inventors:
Jonathan Wang, Neha Shah, Jason Walther, Jiuyi Lu, Timothy Johnson, Yukun Ren, Jean McLarty
Abstract: The present invention provides a composition of nanoparticles comprising a biological mimetic base component that forms the structure of the nanoparticle. By interacting with the functional groups of the base component, the half-life of a bioactive molecule is extended.
Abstract: Provided is a compound which accelerates the enzymatic reaction catalyzed by an oxidase. The present invention provides an oxidase reaction accelerating agent comprising a compound represented by formula (I) and a method using the same.
Abstract: Provided herein are a voltage indicator and a method of measuring voltage. The voltage indicator includes a membrane-localized voltage-sensitive protein coupled to a capture protein. The method of measuring voltage includes administering a voltage indicator including a membrane-localized voltage-sensitive protein coupled to a capture protein, and determining changes in fluorescence of a small-molecule fluorescent dye captured by the capture protein.
Type:
Grant
Filed:
November 30, 2017
Date of Patent:
July 12, 2022
Assignee:
HOWARD HUGHES MEDICAL INSTITUTE
Inventors:
Eric R. Schreiter, Luke D. Lavis, Ahmed Abdelfattah
Abstract: Methods are disclosed for viable preservation of biomaterials including both prokaryotic and eukaryotic cells/materials such as human cells and tissues at subzero and suprazero temperatures. One embodiment provides a method wherein initial desiccation and subsequent cooling of the biological samples is below their glass transition temperature (Tg) to achieve a stable glassy state without exposing the biomaterials to excessive osmotic/chemical stresses for long periods of time. Another embodiment provides a method that includes combining the initial desiccation with subsequent freeze-drying to achieve a glassy state of biomaterials. Another embodiment provides a desiccation medium with low salt, high osmolyte/glass former content and desiccation of biomaterials in a spherical droplet to avoid the edge effect.
Type:
Grant
Filed:
October 2, 2018
Date of Patent:
June 14, 2022
Assignee:
AUGUSTA UNIVERSITY RESEARCH INSTITUTE, INC.
Abstract: A method for the cryopreservation of eggs, nauplii and juvenile of barnacles. Cryopreserved feed and a use of the preserved organisms as live feed organism in marine aquaculture typically replacing rotifers and Artemia and other life feed organisms.
Abstract: The invention includes methods, apparatus, and systems for the conversion of hydrogen together with carbon dioxide or carbon monoxide to methane using a population of microbes and a defined liquid medium. The microbial population maintains the amount of nutrients in the liquid medium within a relative range without requiring replenishment of the nutrients. Methods and apparatus for enriching the microbial population are also described.
Type:
Grant
Filed:
June 29, 2017
Date of Patent:
May 17, 2022
Inventors:
Sandra Esteves, Richard Mark Dinsdale, Timothy Patterson, Savvas Savvas
Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.
Type:
Grant
Filed:
November 20, 2015
Date of Patent:
May 10, 2022
Assignee:
Inguran, LLC
Inventors:
Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
Abstract: Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 ?m to 5 ?m thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The thin regions of parylene are semipermeable to allow for proteins in serum to pass through, and the thick regions give mechanical support for handling by a surgeon. The smooth front side allows for monolayer cell growth, and the rough back side helps prevents cells from attaching there.
Type:
Grant
Filed:
June 16, 2016
Date of Patent:
May 3, 2022
Assignees:
California Institute of Technology, University of Southern California
Abstract: Embodiments of the invention relate generally to protein extraction and, more generally, to bone protein extraction methods that do not require demineralization. In one embodiment, the invention provides a method comprising: mixing a bone sample and a quantity of an extraction buffer comprising: ammonium phosphate dibasic; or ammonium phosphate dibasic and ammonium bicarbonate; or ammonium phosphate dibasic, ammonium bicarbonate, and guanidine HCl; or sodium phosphate dibasic and sodium bicarbonate; or sodium phosphate dibasic, sodium bicarbonate, and guanidine HCl; or potassium phosphate dibasic and potassium bicarbonate; or potassium phosphate dibasic, potassium bicarbonate, and guanidine HCl; and incubating the bone sample/extraction buffer mixture.
Abstract: The present invention provides wood preservative composition containing a dispersed and/or emulsified phase comprising a wood preservative component; and a stabilizer. The stabilizer is a cationic polymer, an enzyme, organic polymer, quaternary ammonium compound and/or a mixture thereof. Also provided is a method of preserving wood or other cellulosic material which comprises applying to the wood or other cellulosic material.
Type:
Grant
Filed:
April 30, 2015
Date of Patent:
April 26, 2022
Assignee:
ARCH WOOD PROTECTION, INC.
Inventors:
Kimberlee Thompson, Susan Thomason, Alex Valcke, David Cantrell
Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.
Type:
Grant
Filed:
March 15, 2021
Date of Patent:
April 19, 2022
Assignee:
Ludwig Institute for Cancer Research Ltd
Abstract: Disclosed is the use of isothiocyanate compounds. In particular, disclosed is the use of a compound as shown by Formula (I) or a derivative as shown by Formula (II) in the manufacture of a composition or preparation for preventing and/or treating hyperlipidemia. The compound of the present invention can reduce the levels of triglycerides and low density lipoproteins in the serum of mammals (such as rats and humans), increase the level of high density lipoproteins in the serum, and reduce the level of total bilirubin in the serum of mammals (such as rats and humans).
Abstract: The present invention relates to a novel process for biological detection of mycobacteria via electrochemical analysis methods of the catalytic activity of antigen 85.
Type:
Grant
Filed:
March 29, 2018
Date of Patent:
April 5, 2022
Assignees:
UNIVERSITÉ DE BOURGOGNE, INSTITUT NATIONAL DE RECHERCHE POUR LAGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Abstract: The present disclosure relates to the provision of novel biodegradable amphiphilic peptides and peptide analogues based derivatives comprising hydrophobic chains and their use in the permeabilization of mammalian cells and delivery of agents, for example therapeutic agents, imaging agents and cell preservation agents.
Abstract: The present disclosure provides methods for determining an etiology of upper and/or lower eyelid puffiness by examining a subject with squinted eyes; and determining if upper and/or lower eyelid puffiness does not improve, improves, partially improves, or worsens, wherein the etiology of the upper and/or lower eyelid puffiness is diagnosed to be anterior to the orbicularis oculi muscle if the puffiness does not improve, wherein the etiology of the upper and/or lower eyelid puffiness is determined to be posterior to the orbicularis oculi muscle if the puffiness improves, wherein the etiology of the upper and/or lower eyelid puffiness is determined to be anterior and posterior to the orbicularis oculi muscle if the puffiness partially improves, or wherein the puffiness is determined to be secondary to hypertrophy of the orbicularis muscle or if the puffiness worsens.
Abstract: An enzymatic processing plant for continuous flow-based enzymatic processing of organic molecules. The enzymatic processing plant including an enzymatic processing area, wherein the enzymatic processing area includes a turbulence-generating pipe with a repeatedly changing centre-line and/or a repeatedly changing cross-section, for generating turbulence to mix a reaction mixture and prevent sedimentation of particles as the reaction mixture is flowing through the turbulence-generating pipe. The enzymatic processing plant and the enzymatic processing area are arranged such that the reaction mixture is subjected to turbulence within the enzymatic processing area for a reaction time of 15 minutes or more.
Abstract: The present application relates to a freeze-dried polymer composition containing chitosan and at least one lyoprotectant, a process for preparing a freeze-dried composition containing chitosan and at least one lyoprotectant and the use of a reconstituted freeze-dried chitosan composition to prepare implants for tissue repair.
Type:
Grant
Filed:
February 20, 2015
Date of Patent:
March 29, 2022
Assignee:
Ortho Regenerative Technologies Inc.
Inventors:
Anik Chevrier, Michael D. Buschmann, Daniel Veilleux, Caroline Hoemann, Marc Lavertu