Patents Examined by Susan M. Weber
  • Patent number: 5436152
    Abstract: Immortalized human epithelial cell sublines are provided. The novel cell lines do not undergo terminal differentiation and senescence upon exposure to high calcium concentrations. The novel cells exhibit positive reactivity with milk-fat globule membrane antigen and cytokeratin anti-serum. The cells are non-tumorigenic in athymic mice, and exhibit both three-dimensional growth in collagen and dome formation in confluent cultures. The cell sublines demonstrate growth control by hormones and growth factors. The novel cell sublines are useful in evaluating the capacity of preselected agents to bring about a change in epithelial cell growth and in the production of proteins.
    Type: Grant
    Filed: May 20, 1991
    Date of Patent: July 25, 1995
    Inventors: Herbert Soule, Charles M. McGrath
  • Patent number: 5292655
    Abstract: Methods and formulations are disclosed for the in vitro formation of a histologically complete human epidermis in a serum-free, companion cell or cell feeder layer-free, and organotypic matrix-free culture system commencing with the isolation and cultivation of a unique population of clonally-competent basal epidermal cells and ending with the formation of a functional, histologically complete, human squamous epithelium. The formation of a histologically complete human epidermis is accomplished in a serum-free medium, without companion-cells or feeder layer cells or any organotypic support using a multi-step process that is controlled by manipulating the growth and differentiation factors requisite to the sequential development of a usable, functional, and completely differentiated epidermis.
    Type: Grant
    Filed: January 29, 1990
    Date of Patent: March 8, 1994
    Inventor: John J. Wille, Jr.
  • Patent number: 5284765
    Abstract: The invention is a method of ordering plant embryos so that a specified end of the embryo is always oriented in a predetermined direction. The embryos are preferably first fairly rapidly desiccated to about 15% moisture content. They are then suspended in a benign liquid flotation medium having a density in the range of about 1.059-1.104 g/cm.sup.3. The density must be adjusted empirically so that a predominant number of viable embryos will float and nonviable embryos will sink. In at least the case of conifer somatic embryos, they will float with the end bearing the latent cotyledons upward. After sufficient separation time in the flotation medium the oriented embryos are swept by a flowing liquid stream into a conduit. They enter cotyledon end first and are then carried to a delivery point without losing that orientation. Here they are separated from the transporting medium.
    Type: Grant
    Filed: April 8, 1992
    Date of Patent: February 8, 1994
    Assignee: Weyerhaeuser Company
    Inventors: Curtis A. Bryan, William C. Carlson, Michael K. McKinnis
  • Patent number: 5279824
    Abstract: A method of treating thrombosis with a pharmaceutical formulation comprising an endo-beta-glucuronidase, preferably leech-derived, heparin and a pharmacologically acceptable diluent, carrier or excipient which does not prevent the interaction between the heparin and the endo-beta-glucuronidase is disclosed. The formulation, which is disclosed, can also comprise a clot-lytic agent such as tissue plasminogen activator. Endo-beta-glucuronidase, unlike other hyaluronidases, has been found not to be inhibited by heparin.
    Type: Grant
    Filed: November 9, 1992
    Date of Patent: January 18, 1994
    Assignee: Merck Patent GmbH
    Inventors: Roy T. Sawyer, Christopher Powell-Jones
  • Patent number: 5277908
    Abstract: A method and compositions for reducing ischemic, inflammatory or septic injury in an animal that includes administration to the animal of an effective amount of a modified superoxide dismutase, wherein the modification comprises substituting a first amino acid residue located within 3-12 .ANG. of the active site of the superoxide dismutase with a second amino acid residue capable of reacting with a highly reactive nitrating species, such as nitronium ion, formed from the reaction of peroxynitrite and superoxide dismutase.
    Type: Grant
    Filed: May 29, 1992
    Date of Patent: January 11, 1994
    Assignee: The UAB Research Foundation
    Inventors: Joseph S. Beckman, Haralambos Ischiropoulos, Craig D. Smith
  • Patent number: 5270448
    Abstract: The invention concerns novel isolates of Bacillus thuringiensis (B.t.) which contain a toxin(s) which is active against nematodes. This B.t. toxin(s) or B.t. isolate(s) can be used to treat animals and plants hosting susceptible nematodes.
    Type: Grant
    Filed: July 21, 1992
    Date of Patent: December 14, 1993
    Assignee: Mycogen Corporation
    Inventor: Jewel M. Payne
  • Patent number: 5262298
    Abstract: Keratinocyte autocrine factor (KAF) can be isolated from human keratinocytes and is an effective and selective mitogen for keratinocytes in comparison to fibroblasts. Methods of purifying KAF using sulfated glycosaminoglycans, methods to inhibit the effect of KAF using sulfated glycosaminoglycans, and methods to assay for stimulators and inhibitors of KAF activity and production are described.
    Type: Grant
    Filed: July 26, 1990
    Date of Patent: November 16, 1993
    Assignee: The State of Oregon Acting by and Through the State Board of Education on Behalf of the Oregon Health Sciences University
    Inventors: Gary D. Shipley, Mark R. Pittelkow, Paul W. Cook
  • Patent number: 5260211
    Abstract: A process and system for cultivating adhesive cells in a packed bed of solid cell matrix is disclosed. Adhesive cells are adhered to a solid cell matrix, preferably ceramic particles, and packed in a cell culture tank to form the packed bed. The culture medium is circulated through the packed bed in a direction. The circulating of the culture medium is then stopped and the direction of the medium being circulated is reversed. This periodic switching of the direction of the culture medium reduces channelling of the culture medium. The bottom or head of the culture tank can also contain a separator to trap any bubble existing in the culture medium or to trap any quantity of cell matrix possibly running out from the culture tank.
    Type: Grant
    Filed: November 26, 1990
    Date of Patent: November 9, 1993
    Assignee: Snow Brand Milk Products Co., Ltd.
    Inventors: Yoshiaki Matsuda, Shinjiro Mitsuda, Eitaro Kumazawa, Atushi Baba, Masakatsu Uesaka
  • Patent number: 5256571
    Abstract: An aqueous alcohol buffer solution for substantially ambient, in vitro preservation of mammalian cells for a selected duration. The solution generally contains a water-miscible alcohol in an amount sufficient to fix the sample cells without coagulation, an anti-clumping agent, and a buffer agent to maintain the solution at a pH within a range of four to seven.
    Type: Grant
    Filed: May 1, 1991
    Date of Patent: October 26, 1993
    Assignee: Cytyc Corporation
    Inventors: Anne A. Hurley, Daniel C. Lapen, Peter S. Oud
  • Patent number: 5244680
    Abstract: Strains of microorganisms including strains of Cryptococcus are described which have the ability to control postharvest wound or surface fruit pathogens, even at the low temperatures at which fruit is stored. The strains are highly effective in controlling postharvest diseases including mucor rot, blue mold, and gray mold in fruit.
    Type: Grant
    Filed: July 13, 1992
    Date of Patent: September 14, 1993
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventor: Rodney G. Roberts
  • Patent number: 5238840
    Abstract: Two immortalized human mammary epithelial cell sublines are provided. The sublines do not undergo terminal differentiation and senescence upon exposure to high calcium concentrations. The sublines exhibit positive reactivity with milk-fat globule membrane and cytokeratin anti-sera and are non-tumorigenic in athymic mice. The sublines are useful in evaluating the capacity of preselected agents to bring about a change in epithelial cell growth and in the production of proteins.
    Type: Grant
    Filed: July 9, 1991
    Date of Patent: August 24, 1993
    Assignee: Michigan Cancer Foundation
    Inventors: Robert J. Pauley, Terry J. Paine, Herbert D. Soule
  • Patent number: 5232848
    Abstract: This defined basal nutrient medium is very effective in both high and low density culture of a wide variety of cell lines and cell types. The medium may be used for serum-free culture or supplemented with low levels of serum. The medium contains a buffer system formulated for air equilibration.
    Type: Grant
    Filed: November 16, 1989
    Date of Patent: August 3, 1993
    Assignee: W. R. Grace & Co.-Conn.
    Inventors: Richard A. Wolfe, Aaron H. Heifetz, Linda M. Custer
  • Patent number: 5231013
    Abstract: A new polycyclic peptide antibiotic termed gallidermin is disclosed and described, together with a process for producing such antibiotic by fermination of a new microorganism, Staphylococcus gallinarum (DSM 4616).
    Type: Grant
    Filed: May 18, 1989
    Date of Patent: July 27, 1993
    Inventors: Gunther Jung, Roland Kellner, Hans Zahner, Friedrich Gotz, Thomas Horner, Rolf G. Werner, Hermann Allgaier
  • Patent number: 5225343
    Abstract: A process for the preparation of an adventive embryo of a plant belonging to the genus Podophyllum, comprising the steps of preparing cells of a plant belonging to the genus Podophyllum, incubating the cells in a sugar free medium or a medium containing up to 2% by weight sugar, and then incubating the cells in a medium containing more than 2% by weight of sugar to generate an adventive embryo; a process for the production of a podophyllotoxin compound comprising the steps of preparing an adventive embryo of a plant belonging to the genus Podophyllum, culturing the adventive embryo to generate an adventitious organ, culturing the adventitious organ to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound; and a process for the production of a podophyllotoxin compound, comprising the steps of preparing de-differentiated cells or tissue of a plant belonging to the genus Podophyllum culturing the de-differentiated cells or tissue in a liquid medium to generate a differentiated organ, cu
    Type: Grant
    Filed: October 26, 1990
    Date of Patent: July 6, 1993
    Assignee: Nippon Oil Company, Ltd.
    Inventors: Ko Sakata, Eitaroh Morita, Tetsuya Takezono
  • Patent number: 5223491
    Abstract: A method revitalizing skin by applying topically a substantially purified water insoluble glucan, extracted from yeast cell walls, dispersed within a carrier. Also, disclosed is a method for producing the water insoluble glucan.
    Type: Grant
    Filed: November 9, 1989
    Date of Patent: June 29, 1993
    Inventor: Byron A. Donzis
  • Patent number: 5223430
    Abstract: This disclosure describes novel antibacterial agents designated LL-E10985.alpha., LL-E19085.beta., LL-E19085.gamma., LL-E19085.zeta. and LL-E19085.eta., having the structure: ##STR1## their production by aerobic fermentation of a new subspecies of Micromonospora citrea NRRL 18351 and mutants thereof, and the isolation and purification thereof.
    Type: Grant
    Filed: October 28, 1991
    Date of Patent: June 29, 1993
    Assignee: American Cyanamid Company
    Inventors: Guy T. Carter, Joseph J. Goodman, David P. Labeda
  • Patent number: 5221622
    Abstract: A novel 170 kD membrane protease is isolated from malignant human melanoma cell line LOX and RPMI7951. The protease is useful in a method of diagnosing cellular transformation.
    Type: Grant
    Filed: October 11, 1990
    Date of Patent: June 22, 1993
    Assignee: The Board of Regents of Georgetown University
    Inventor: Wen-Tien Chen
  • Patent number: 5217892
    Abstract: A method of plant tissue culture which comprises culturing a tissue or an organ of a plant, a part of the same or cultured cells in a medium containing a culture filtrate and/or an extract of a photosynthetic procaryotic microorganism.Such a culturing method effectively proliferates the plant tissues and cultured cells, and also promotes the formation of adventive embryos, regeneration of the plant body and production of useful substances formed by that plant.Strains of cyanobacteria or photosynthetic bacteria are preferably used as the photosynthetic procaryotic microorganism.
    Type: Grant
    Filed: July 8, 1992
    Date of Patent: June 8, 1993
    Assignees: Pentel Kabushiki Kaisha, Tadashi Matsunaga
    Inventors: Tadashi Matsunaga, Hitoshi Wake, Mayumi Ono, Kiyoshi Hishinuma, Hironori Umetsu
  • Patent number: 5208159
    Abstract: Two strains of chitinolytic bacteria respectively named "Corynebacterium Bine-A" "Achromobacter Bine-B", which were deposited under deposit numbers FERM BP-2879 and FERM BP-2880 in the Fermentation Research Institute, Agency of Industrial Science and Technology, Japan. At least one of these strains is incubated on a chitin or chitosan medium, to prepare a culture material, which is usable directly as a solid antibacterial composition or fermented or aged to produce a solid antibacterial composition. Alternatively, water is added to the prepared culture material, and either of the liquid and solid phases may be used as an antibacterial, anti-nematode, and/or plant-cell activating composition.
    Type: Grant
    Filed: May 15, 1990
    Date of Patent: May 4, 1993
    Assignee: Toda Biosystem Laboratory
    Inventors: Takashi Toda, Hideyuki Matsuda
  • Patent number: 5206165
    Abstract: Two immortalized human mammary epithelial cell sublines are provided. The sublines do not undergo terminal differentiation and senescence upon exposure to high calcium concentrations. The sublines exhibit positive reactivity with milk-fat globule membrane and cytokeratin anti-sera and are non-tumorigenic in athymic mice. The sublines are useful in evaluating the capacity of preselected agents to bring about a change in epithelial cell growth and in the production of proteins.
    Type: Grant
    Filed: July 9, 1991
    Date of Patent: April 27, 1993
    Assignee: Michigan Cancer Foundation
    Inventors: Robert J. Pauley, Terry J. Paine, Herbert D. Soule