Patents Examined by William H. Beisner
  • Patent number: 10914722
    Abstract: A sensor array for simultaneously measuring electrophysiological responses and contractility responses of a tissue includes: a substrate; a multi-electrode array (MEA) disposed in or on the substrate; and a plurality of interdigitized electrodes (IDEs) disposed in or on the substrate. The MEA and the IDEs are interpenetrating within a plane substantially parallel to an upper surface of the substrate. Systems for measuring such responses using the sensor array may also include contacts and/or connectors for interfacing with external control devices, electrodes, and cell culture components such as a chamber and lid. Fabrication techniques for making such systems include: forming a sensor array comprising an interpenetrating arrangement of IDEs and electrodes of a MEA in or on a substrate surface; forming a plurality of contacts for interfacing the system with one or more external devices in or on the substrate surface; and forming leads between the plurality of contacts and the sensor array.
    Type: Grant
    Filed: February 21, 2018
    Date of Patent: February 9, 2021
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: Fang Qian, Chao Huang, Anna Nikolaevna Ivanovskaya, Kris Kulp, Elizabeth K. Wheeler
  • Patent number: 10913930
    Abstract: A tissue treatment apparatus and method for treating bone tissue has a controller, an enclosure, a reagent supply system, a draining configuration, and a gas relief valve is controlled by the controller. A bioactive agent may be applied through the reagent supply system. Optionally provided is a gas evacuation assembly, a gas supply unit, a thermal unit for heating or cooling reagents or gases, a sonication unit, any or each operated by the controller. The method provides for programming controller to effect a treatment procedure. In an embodiment of the present application the treatment procedure effects demineralization of the bone material.
    Type: Grant
    Filed: August 9, 2016
    Date of Patent: February 9, 2021
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: Subhabrata Bhattacharyya, David R. Kaes, Daniel A. Shimko
  • Patent number: 10913926
    Abstract: An automatic processing device of culture plates (2) for microbiological samples, wherein the processing device (1) includes a support frame (3); a slide (4) provided with a seating (5) configured for removably housing a culture plate (2) and movably mounted on the support frame (3) so as to be selectively displaceable between a first loading position, a plurality of image-acquiring positions, and a first unloading; a camera (6) of a linear type, provided with an optic (7) of a telecentric type and a trilinear sensor, and arranged according to a vertical axis (8) such as to acquire, at an image-acquiring zone, a multiplicity of linear images of corresponding linear portions of an upper surface of the culture plate (2), during the displacing of the slide (4); a first lighting device (11) orientated such as to illuminate the linear portions of an upper surface of the culture plate (2); an advancing device (14) of the slide (4) configured such as to enable obtaining a constant and substantially vibration-free ad
    Type: Grant
    Filed: January 10, 2020
    Date of Patent: February 9, 2021
    Assignee: Copan Italia S.p.A.
    Inventor: Daniele Triva
  • Patent number: 10907125
    Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
    Type: Grant
    Filed: June 17, 2020
    Date of Patent: February 2, 2021
    Assignee: Inscripta, Inc.
    Inventors: Nathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
  • Patent number: 10900008
    Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: January 26, 2021
    Inventors: Paul Vulto, Sebastiaan Johannes Trietsch, Heiko Jan van der Linden, Adrianus Theodoras Joore, Thomas Hankemeier
  • Patent number: 10900005
    Abstract: The disclosure relates to a liquid reservoir for a food waste recycling appliance including a reservoir body with a hollow interior, a first arm portion, and a second arm portion, and at least partially bounding an interstitial space exterior of the reservoir body. A food waste through opening can be defined by an exterior surface of the reservoir body.
    Type: Grant
    Filed: July 24, 2019
    Date of Patent: January 26, 2021
    Assignee: Whirlpool Corporation
    Inventors: Akshay Bhandari, Thomas L. Burger, Rachel Maghas
  • Patent number: 10900012
    Abstract: A probe assembly and a method of manufacturing a probe assembly. In one aspect there is a method of manufacturing a probe assembly comprising providing an electrode carrier carrying a plurality of electrodes, the electrode carrier comprising a top wherein the plurality of electrodes are exposed relative to the top and a bottom having a plurality of electrical contacts in electrical communication with the plurality of electrodes respectively; moulding a body around the electrode carrier to retain the electrode carrier whilst leaving the plurality of electrodes exposed. The invention also extends to a biomass monitoring system comprising a flexible enclosure including a probe assembly and support arrangement for receipt of the probe assembly in an engaged configuration.
    Type: Grant
    Filed: April 22, 2017
    Date of Patent: January 26, 2021
    Inventor: Paul Yeomans
  • Patent number: 10895546
    Abstract: The present disclosure relates to a measuring device with a bipolar electrode array for the impedimetric analysis of adherent cells according to the ECIS principle (electric cells substrate impedance sensing). The measuring device comprises an electrode array which is adapted for being wetted with an electrolyte solution and adherently growing cells in order to perform impedimetric cell analyzes, characterized in that the electrode array comprises a bipolar electrode on a substrate, where the bipolar electrode is formed as a conductive path on the transparent substrate and has an inherent resistance between two connection points of the conductive path that is a multiple of the AC impedance of the electrolyte solution at 1 MHz, measured at the two connection points.
    Type: Grant
    Filed: December 12, 2017
    Date of Patent: January 19, 2021
    Inventors: Christian Goetz, Joachim Wegener
  • Patent number: 10883077
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: March 31, 2020
    Date of Patent: January 5, 2021
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier
  • Patent number: 10883076
    Abstract: The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
    Type: Grant
    Filed: November 13, 2018
    Date of Patent: January 5, 2021
    Assignee: Lonza Biologies PLC
    Inventor: Mohsan Khan
  • Patent number: 10883078
    Abstract: A culture device supplying an accurate amount of liquid into a culture bag has a culture bag having ports, a first bag holding portion having supporting surfaces supporting the culture bag, a rotation mechanism rotating the first bag holding portion, a liquid supplying mechanism supplying a liquid through a tube communicating with the ports a weight detector detecting the weight of the culture bag and the first bag holding portion, and a control portion, in which the control portion sets a first reference value according to a first detection information output from the weight detector in a first state and carries out a liquid supplying step of stopping the liquid supplying mechanism under the condition where a second detection information output from the weight detector when a liquid is supplied to the culturing bag reaches a first target value obtained by adding the weight of the liquid to be supplied to the first reference value.
    Type: Grant
    Filed: May 24, 2016
    Date of Patent: January 5, 2021
    Inventors: Norihisa Sasayama, Masakatsu Takeuchi, Atsushi Taguchi
  • Patent number: 10870823
    Abstract: Described herein are apparatus comprising: a first layer comprising a first microfluidic channel; a second layer comprising a second microfluidic channel; and a membrane for culturing cells; along with methods of making and using same.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: December 22, 2020
    Inventors: Yaling Liu, Antony Thomas
  • Patent number: 10851338
    Abstract: Bio-reactive systems for voltage controlled metabolism are described, that include electrochemical-electrostatic systems having a conventional three electrode cell modified with at least one additional gating electrode. The rate of a metabolic process occurring in at least one organism disposed on a working electrode is controllable by applying a gating voltage VG to the at least one gating electrode. A method for voltage controlled metabolism in a bio-reactive electrostatic cell that includes applying a gating voltage VG to at least one gating electrode is also described. The rate of a metabolic process may be controlled by altering at least one of the magnitude and polarity of the applied gating voltage VG. The method for voltage controlled metabolism may further be used to treat cancer and/or increase the rate of ethanol production by fermentation.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: December 1, 2020
    Inventor: Siu-Tung Yau
  • Patent number: 10850283
    Abstract: The invention relates to a thermal cycler (10) comprising a housing (12), the housing (12) accommodating a thermal block (14) having a plurality of sample wells (32), each for receiving a test sample in a sample vessel, electric heater means (18) for heating the thermal block (14), a power supply (24) and an electronic control (22) for controlling the electric heater means (18), and further comprising a temperature analysis and/or verification unit (28) for analyzing and/or verifying a thermal performance of the thermal block (14). The invention further relates to a method for analyzing or verifying a thermal performance of a thermal cycler (10) and for calibrating the thermal cycler (10).
    Type: Grant
    Filed: December 30, 2018
    Date of Patent: December 1, 2020
    Inventor: James Courtney
  • Patent number: 10844364
    Abstract: A system for accelerating the migration of cells by applying a time-varying magnetic field to induce eddy currents that promote electrotaxis (galvanotaxis) of cells. The system of the present invention accelerates the healing of wounds by electrotaxis of cells.
    Type: Grant
    Filed: October 2, 2017
    Date of Patent: November 24, 2020
    Assignee: Ohio State Innovation Foundation
    Inventors: Vish Subramaniam, Joseph West, Emily Alkandry, Sashwati Roy, Chandan Sen, Piya Ghatak
  • Patent number: 10844343
    Abstract: Disclosed is a device for the electrical disintegration of cell structures including a chamber having an inlet for receiving material containing cell structures, an outlet for the discharge thereof, and a conveyor line extending between the inlet and the outlet, an electrode unit which has an electrode body which is arranged within the chamber at least portion-wise along the conveyor line, wherein the chamber has a wall which is portion-wise or completely electrically conductive and electrically insulated from the electrode body, and the electrode unit is adapted to generate an electric field between the electrode body and the wall for electrical disintegration.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: November 24, 2020
    Assignee: Hugo Vogelsang Maschinenbau GmbH
    Inventors: Clemens Hollah, Paul Krampe, Andreas Fastenau
  • Patent number: 10843195
    Abstract: Provided herein are devices, systems, and methods for specimen preparation by employing a combination of capillary and centrifugal forces. For example, provided herein are devices, systems, and methods that collect a sample by capillary force, separate components of the collected sample by centrifugal force, and isolate one or more of the separated components by further capillary force.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: November 24, 2020
    Assignee: Northwestern University
    Inventors: David M. Kelso, Robert Elghanian
  • Patent number: 10834920
    Abstract: The present invention is directed to a vitrification stick for use in the cryopreservation of biological materials, and may include a body having a first portion and a second portion, and a specimen end extending from the second portion of the body, and having a basket end tip formed thereon. The basket end tip may include a peripheral wall enclosing an interior region of the basket end tip, and the interior region may be further enclosed on a first side by a shell connected to the peripheral wall, and the interior region may be open on a second side in a direction towards the specimen end. The shell may include at least one slot formed therein.
    Type: Grant
    Filed: January 13, 2020
    Date of Patent: November 17, 2020
    Inventor: Lee L. Nemeth
  • Patent number: 10836988
    Abstract: A customizable laboratory instrument comprising an interior space for receipt of test materials therein. An indented exterior surface of the laboratory instrument defines a boundary surface, a backing surface, and a sidewall. The backing surface is offset from the boundary surface a distance substantially equal to a depth of the sidewall. A panel having an edge is attachable to the sidewall such that a space is formed between the panel and backing surface for receipt of an object therein.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: November 17, 2020
    Inventors: Scott Shindledecker, Edward Skevington, Brent Ronald Pohl, Dustin Diemert, Thierry Guillet
  • Patent number: 10837953
    Abstract: According to one embodiment, a sensor includes a nonmagnetic layer and a plurality of magnetic field sensors. The nonmagnetic layer has a first surface and a second surface. The magnetic field sensors are arranged along the second surface. The second surface is between the first surface and the magnetic field sensors. Each of the magnetic field sensors includes a first magnetic layer, a second magnetic layer provided between the first magnetic layer and the nonmagnetic layer, and an intermediate layer provided between the first magnetic layer and the second magnetic layer. The intermediate layer is nonmagnetic. A distance between the first surface and the second magnetic layer is not more than a pitch of the magnetic field sensors.
    Type: Grant
    Filed: February 22, 2017
    Date of Patent: November 17, 2020
    Assignee: Kabushiki Kaisha Toshiba
    Inventors: Akira Kikitsu, Hitoshi Iwasaki