Patents Examined by William H. Beisner
  • Patent number: 10731120
    Abstract: The present invention discloses a flow electroporation device comprising a three-dimensional electrode device and a flow electroporation chamber, the three-dimensional electrode device comprising an electrode array and an electrode fixing assembly for fixing electrodes in the electrode array, the flow electroporation chamber comprising a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening on the cavity, and the electrode array being inserted into the cavity through the opening By means of the above-mentioned manner, the flow electroporation device of the present invention can achieve a high throughput cell treatment, and also can work under a low voltage condition, can avoid damages to cells brought by liquid flow, can reduce effects brought by heat and cathode effect induced by electric pulses, and can maintain an closed aseptic environment.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: August 4, 2020
    Assignee: ETTA BIOTECH CO., LTD.
    Inventor: Edward Dai
  • Patent number: 10730808
    Abstract: The present invention relates to a self-deodorizing composting system, comprising an outer membrane, an inner membrane, an air-ribbed cage and a deodorizing base, wherein two deodorizing bases are provided; the outer membrane and the inner membrane are held by the air-ribbed cage, with both ends of the outer membrane and the inner membrane respectively fixed to the two deodorizing bases, whereby a closed space is formed between the outer membrane and the inner membrane; the inner side of the inner membrane is used for placing the composting piles, with the malodor and water vapor generated from the composting piles entering into the closed space formed between the outer membrane and the inner membrane through the inner membrane, so that the water vapor is condensed into the water in the closed space, and the malodor molecules are absorbed and dissolved into the condensed water, thus forming the waste water which falls along the inner side of the outer membrane and the outer side of the inner membrane to the b
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: August 4, 2020
    Inventors: Fan Lyu, Pinjing He, Liming Shao, Hua Zhang
  • Patent number: 10731117
    Abstract: The present invention relates to a solid state fermentation process for producing hydrogen, and to a bioreactor and solid support for use in the fermentation process.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: August 4, 2020
    Assignee: Q POWER OY
    Inventors: Anni Alitalo, Erkki Aura, Marko Niskanen
  • Patent number: 10723989
    Abstract: The invention relates to a container device (1), comprising: a container receiving portion (4) for receiving a container; at least one valve (6) for controlling a flow of fluid in a fluid line connected to said container; a pump device (8) for generating a conveyor pressure of the fluid in the fluid line; a control device (10) which can be connected to the valve (6) and/or the pump device (8) in order to control the valve (6) and/or pump device (8); and a frame (2) which supports said container receiving portion (4), valve (6), pump device (8) and control device (10).
    Type: Grant
    Filed: May 12, 2016
    Date of Patent: July 28, 2020
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Jan Schaefer, Christian Manzke, Etienne Evrard
  • Patent number: 10717961
    Abstract: A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.
    Type: Grant
    Filed: April 24, 2013
    Date of Patent: July 21, 2020
    Inventors: Ushio Iwamoto, Michi Sato, Kanako Konishi, Katsuhisa Matsuura, Tatsuya Shimizu, Teruo Okano
  • Patent number: 10717959
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: July 21, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier
  • Patent number: 10710080
    Abstract: Provided is a container for PCR enabling cell observation and a PCR treatment with a single container. In the container for PCR, a bottom surface 17a of an inside is flat, a shape of the bottom surface is a circular shape or a polygonal shape with four or more sides, a size of the bottom surface 17a is large so that a diameter L of an approximated circle circumscribing the bottom surface 17a is 0.05 mm? or more and 1 mm? or less, and an angle of an angle ? on a side surface side formed between a side surface 17b adjacent to the bottom surface 17a and the bottom surface 17a is 50° or more and 80° or less.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: July 14, 2020
    Assignee: FUJIFILM Corporation
    Inventor: Yasuhisa Kaneko
  • Patent number: 10703683
    Abstract: A facility for treating and recycling animal waste (2), including a unit (6) for methanizing the waste including treatment of the obtained biogases, a cogeneration unit (8) delivering electricity and heat (32) from the biogases, and a unit for hydroponically cultivating microalgae (14) in photo-bioreactors supplied by the liquid phase (12) of the organic residues from the methanization. The facility further includes a unit for cultivating macrophytes (22) supplied with water (20) leaving the unit for cultivating microalgae, and a vermiculture unit (24) fed by harvesting the macrophytes (22) and by the sludge (36) coming from the raw digestate from the methanization.
    Type: Grant
    Filed: June 30, 2014
    Date of Patent: July 7, 2020
    Inventor: René-Jean Guillard
  • Patent number: 10704018
    Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.
    Type: Grant
    Filed: July 30, 2019
    Date of Patent: July 7, 2020
    Inventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
  • Patent number: 10690677
    Abstract: Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: June 23, 2020
    Assignee: ACEA Biosciences, Inc.
    Inventors: Xiao Xu, Yama A. Abassi, Xiaobo Wang, Jiangbo Gan
  • Patent number: 10683476
    Abstract: A bioreactor system for preparing a cardiac organoid chamber and for subsequent testing thereof is described herein and shown in the exemplary drawing figures. The bioreactor system includes a first vessel having a hollow interior and an open top. A first cover is mated with the open top of the first vessel. The first cover has a first opening formed therein. The system further includes a cannula having a lumen that extends from an open first end to an open second end. The cannula is disposed within the first opening of the first cover such that a portion of the cannula lies below the first cover and for insertion into the hollow interior of the first vessel. A porous ring is coupled to the cannula at or proximate the open second end thereof. The system also includes a balloon catheter having an inflatable balloon at a distal end of a catheter shaft. The balloon catheter is adapted to pass through the lumen of the cannula when the balloon is in a deflated state.
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: June 16, 2020
    Assignee: Icahn School of Medicine at Mount Sinai
    Inventors: Kevin David Costa, Timothy James Cashman, Peter Constantine Backeris
  • Patent number: 10684276
    Abstract: Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: June 16, 2020
    Assignee: Hitachi High-Tech Corporation
    Inventors: Ryosuke Takahashi, Katsuhiro Kanda, Yusuke Shimizu, Hisashi Ichikawa
  • Patent number: 10676705
    Abstract: The invention provides an automated apparatus of cell culture having tanks of culture medium, of growth factors and of cells to be cultured, an incubator having a thermostated enclosure which houses a cell culture vessel, and control computer system. A supporting and agitation device of the culture vessel is provided in the enclosure, and the culture vessel is formed by a bag having at least one inlet port connected to the tanks and one outlet port connected devices for harvesting and storage of the cells after culture, these harvesting and storage devices and tanks being located outside the enclosure and being connected to the cell expansion bag ports by conduits which together with the cell expansion bag form a preassembled module passing through a wall of the enclosure.
    Type: Grant
    Filed: June 8, 2012
    Date of Patent: June 9, 2020
    Assignee: CELLPROTHERA
    Inventors: Philippe Henon, Claire Saucourt, Patrick Gasse, Alain Sundas, Pierre Sugranes, Amandine Verdier, Frederic Demonchy
  • Patent number: 10669517
    Abstract: The present invention relates to an apparatus and a method that can accelerate reproduction of odor from an air-conditioner by segmenting microorganisms, a temperature/humidity condition and nutrients for metabolism of the microorganisms as conditions for reproducing the odor from the air-conditioner and setting the segmented microorganisms, a temperature/humidity condition and nutrients for metabolism of the microorganisms according to an accelerated condition thereof.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: June 2, 2020
    Assignees: Hyundai Motor Company, Kia Motors Corporation
    Inventors: Tae Hee Lee, Ki Young Yoon, Jae Sik Choi, Ji Wan Kim, So Yoon Park
  • Patent number: 10669516
    Abstract: A bioreactor system for conditioning of pluripotent cells or cell media is provided. In further aspects, conditioned pluripotent cells and methods for making such cells are provided.
    Type: Grant
    Filed: June 23, 2016
    Date of Patent: June 2, 2020
    Inventors: Charles S. Cox, Jr., Brijesh S. Gill, Kevin Aroom, Pamela Wenzel
  • Patent number: 10663989
    Abstract: A micro channel device processing apparatus includes a heating/cooling chamber configured to receive at least a sub-portion of a micro channel device and a fluid control system that controls a flow of a heating/cooling fluid in the chamber. A method includes controlling a temperature of a sample carried by a micro channel device installed in a micro channel device processing apparatus via a heating/cooling chamber of the processing apparatus. A micro channel device processing apparatus includes a heating/cooling chamber configured to receive a micro channel device carrying a sample and means for controlling a temperature of the sample in the chamber.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: May 26, 2020
    Assignee: Analogic Corporation
    Inventor: Louis Poulo
  • Patent number: 10655159
    Abstract: A locating method and system of determining a microorganism concentration of a fluid in a Petri dish is disclosed. The Petri dish includes a culture medium on which a seeding device is rotatable relative to the Petri dish. The seeding device distributes the fluid and includes at least one point of contact with the culture medium. The point of contact is associated with a contact zone.
    Type: Grant
    Filed: March 11, 2019
    Date of Patent: May 19, 2020
    Assignee: BIOMERIEUX
    Inventors: Laurent Drazek, Corine Fulchiron, Frederic Pinston, Herve Rostaing
  • Patent number: 10626358
    Abstract: The present invention provides for methods and at least partially automated devices suitable for producing a transplantable cellular suspension of living tissue suitable for promoting tissue regeneration in an epithelium-related procedure, as well as compositions produced therefrom. Tissue regeneration in humans is extremely limited and constitutes a major challenge to the repair of damaged organ function. Wound treatment is a typical area where tissue regeneration is required. Wounds (lacerations or openings) in mammalian tissue can result in tissue disruption and coagulation of the microvasculature at the wound face.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 21, 2020
    Inventors: Andrew Perry Quick, David Allen Fencil, William Ford Dolphin
  • Patent number: 10610910
    Abstract: A waste disposal apparatus for disposing of waste materials using aerobic decomposition includes a decomposition chamber, a waste inlet configured to admit waste materials into the decomposition chamber, a water feed system configured for supplying feed water to the decomposition chamber and a drain system configured for draining drain liquid from the decomposition chamber. The apparatus also includes a sensing system configured to sense the volume VF of feed water supplied to the decomposition chamber and the volume VD of drain liquid drained from the decomposition chamber, and a computing device that is configured to determine the quantity of waste material processed by the waste disposal apparatus from the sensed volumes VF and VD.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: April 7, 2020
    Assignee: Mechline Developments Limited
    Inventor: Peter Sage-Passant
  • Patent number: 10611699
    Abstract: A method of determining the volume of organic material in a composting device includes rotating a mixing paddle in a composting bin that stores the organic material; monitoring the torque from a motor that rotates the mixing paddle as the mixing paddle rotates; and determining the height of the organic material in the composting bin based on the motor torque and the angular displacement of the mixing paddle. The resulting height can be used to determine the volume of the organic material.
    Type: Grant
    Filed: November 17, 2016
    Date of Patent: April 7, 2020
    Inventors: Alexander Joseph Halbleib, Gregory Samuel Lieto, Matthew MacLean