Patents by Inventor Adam Ezra Cohen
Adam Ezra Cohen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230168480Abstract: High-speed optical targeting systems and methods are described, wherein a light source, e.g., a laser, is optically coupled with a spatial light modulator. Some embodiments include a device for two-dimensional light steering. In some embodiments, the device comprises a spatial light modulator, and a laser in optical communication with the spatial light modulator. In some exemplary methods, an area is scanned withing a microscope with millisecond revisit time, such as with at least 500 individually targeted points of light. In other exemplary methods, a beam of light is directed from a laser light source into an optical system, through which the light may be focused into a line on a spatial light modulator, wherein the light can be scanned across the spatial light modulator, and directed from the spatial light modulator onto a sample. Other exemplary methods are drawn to the construction and use of the embodiments escribed herein.Type: ApplicationFiled: April 21, 2021Publication date: June 1, 2023Applicant: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Shane Nichols, Vicente Jose Parot, Hunter Davis Ozawa
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Publication number: 20230151437Abstract: The present disclosure provides methods for profiling cellular events in a cell over time using protein assemblies. Methods for profiling the effect of a stimulus, such as a candidate therapeutic agent, are also provided by the present disclosure. Proteins, polynucleotides (e.g., vectors), and pairs of polynucleotides for use in the methods described herein, as well as systems and kits comprising such proteins, polynucleotides, and pairs of polynucleotides, are also provided herein.Type: ApplicationFiled: October 12, 2022Publication date: May 18, 2023Applicant: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Dingchang Lin, Xiuyuan E. Li, Doyeon Kim
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Publication number: 20220214279Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: ApplicationFiled: March 25, 2022Publication date: July 7, 2022Applicant: Quantum-Si IncorporatedInventors: JONATHAN M. ROTHBERG, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Ciperiany, Jack Jewell, Lawrence C. West, Michael Ferrigno, Paul E. Glenn, Adam Ezra Cohen, Anthony Bellofiore
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Publication number: 20220205919Abstract: The present disclosure relates to spatially modulating the light source used in microscopy. In some cases, a light source projects a sequence of two-dimensional spatial patterns onto a sample using a spatial light modulator. In some cases, the spatial patterns are based on Hadamard matrices. In some cases, an imaging device captures frames of image data in response to light emitted by the sample and orthogonal components of the image data are analyzed by cross-correlating the image data with the spatial pattern associated with each frame. A microscope may be calibrated by illuminating a sample with the sequence of spatial patterns, capturing image data, and storing calibration that maps each pixel of the spatial light modulator to at least one pixel of the imaging device.Type: ApplicationFiled: January 4, 2022Publication date: June 30, 2022Applicant: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Vicente Jose Parot
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Publication number: 20220170861Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: ApplicationFiled: February 18, 2022Publication date: June 2, 2022Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Cipriany, Jack Jewell, Lawrence C. West, Michael Ferrigno, Paul E. Glenn, Adam Ezra Cohen, Anthony Bellofiore
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Patent number: 11287382Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: GrantFiled: November 19, 2019Date of Patent: March 29, 2022Assignee: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Cipriany, Jack Jewell, Lawrence C. West, Michael Ferrigno, Paul E. Glenn, Adam Ezra Cohen, Anthony Bellofiore
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Patent number: 11237109Abstract: The present disclosure relates to spatially modulating the light source used in microscopy. In some cases, a light source projects a sequence of two-dimensional spatial patterns onto a sample using a spatial light modulator. In some cases, the spatial patterns are based on Hadamard matrices. In some cases, an imaging device captures frames of image data in response to light emitted by the sample and orthogonal components of the image data are analyzed by cross-correlating the image data with the spatial pattern associated with each frame. A microscope may be calibrated by illuminating a sample with the sequence of spatial patterns, capturing image data, and storing calibration that maps each pixel of the spatial light modulator to at least one pixel of the imaging device.Type: GrantFiled: January 26, 2018Date of Patent: February 1, 2022Assignee: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Vicente Jose Parot
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Patent number: 10800829Abstract: Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.Type: GrantFiled: October 16, 2019Date of Patent: October 13, 2020Assignees: President and Fellows of Harvard College, The Governors of the University of AlbertaInventors: Adam Ezra Cohen, Daniel Hochbaum, Peng Zou, Samouil Leon Farhi, Robert Earl Campbell, Yongxin Zhao, Daniel Jed Harrison
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Publication number: 20200123218Abstract: Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.Type: ApplicationFiled: October 16, 2019Publication date: April 23, 2020Applicants: President and Fellows of Harvard College, The Governors of the University of AlbertaInventors: Adam Ezra Cohen, Daniel Hochbaum, Peng Zou, Samouil Leon Farhi, Robert Earl Campbell, Yongxin Zhao, Daniel Jed Harrison
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Publication number: 20200088643Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: ApplicationFiled: November 19, 2019Publication date: March 19, 2020Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Cipriany, Jack Jewell, Lawrence C. West, Michael Ferrigno, Paul E. Glenn, Adam Ezra Cohen, Anthony Bellofiore
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Patent number: 10533945Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: GrantFiled: November 10, 2017Date of Patent: January 14, 2020Assignee: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Cipriany, Jack Jewell, Lawrence C. West, Michael Ferrigno, Paul E. Glenn, Adam Ezra Cohen, Anthony Bellofiore
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Publication number: 20190391078Abstract: The present disclosure relates to spatially modulating the light source used in microscopy. In some cases, a light source projects a sequence of two-dimensional spatial patterns onto a sample using a spatial light modulator. In some cases, the spatial patterns are based on Hadamard matrices. In some cases, an imaging device captures frames of image data in response to light emitted by the sample and orthogonal components of the image data are analyzed by cross-correlating the image data with the spatial pattern associated with each frame. A microscope may be calibrated by illuminating a sample with the sequence of spatial patterns, capturing image data, and storing calibration that maps each pixel of the spatial light modulator to at least one pixel of the imaging device.Type: ApplicationFiled: January 26, 2018Publication date: December 26, 2019Applicant: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Vicente Jose Parot
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Patent number: 10352945Abstract: The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell.Type: GrantFiled: October 13, 2017Date of Patent: July 16, 2019Assignee: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Joel Kralj, Adam D. Douglass
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Patent number: 10161937Abstract: The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.Type: GrantFiled: July 10, 2017Date of Patent: December 25, 2018Assignee: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Joel Kralj, Adam D. Douglass, Daniel Hochbaum
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Publication number: 20180088052Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: ApplicationFiled: November 10, 2017Publication date: March 29, 2018Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Cipriany, Jack Jewell, Lawrence C. West, Michael Ferrigno, Paul E. Glenn, Adam Ezra Cohen, Anthony Bellofiore
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Publication number: 20180031572Abstract: The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell.Type: ApplicationFiled: October 13, 2017Publication date: February 1, 2018Applicant: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Joel Kralj, Adam D. Douglass
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Publication number: 20180031553Abstract: The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.Type: ApplicationFiled: July 10, 2017Publication date: February 1, 2018Applicant: President and Fellows of Harvard CollegeInventors: Adam Ezra Cohen, Joel Kralj, Adam D. Douglass, Daniel Hochbaum