Abstract: Aspects herein are directed to an apparel item that promotes thermo-regulation through the use of engineered openings, venting, and/or stand-off structures. In exemplary aspects, 20-45% of the apparel item may comprise the engineered openings. Vents may be positioned on the apparel item in areas that experience high amounts of air flow to help channel air into the apparel item. The stand-off structures may be positioned on an inner-facing surface of the apparel item where they help to create a space between the apparel item and the wearer's body surface in which air can flow and help cool the wearer by promoting evaporative cooling.

Abstract: A surface cleaning head includes a housing having a front side and back side, a brush roll rotatably mounted to the housing within a suction conduit and having at least a portion proximate the opening of the suction conduit, a leading roller mounted to the housing in front of the brush roll, and a drive mechanism operatively coupled to the brush roll and the leading roller for driving the brush roll and the leading roller at same time. The brush roll includes an agitator body and a first bristle/flap arrangement comprising a first deformable flap extending from the agitator body and a first bristle strip and/or row of tufts extending from the agitator body and disposed adjacent to the first deformable flap. The first deformable flap is disposed at an aggressive angle and the first bristle strip and/or row of tufts is arranged at a passive angle.

Abstract: A robotic cleaner includes a housing, a suction conduit with an opening, and a leading roller mounted in front of a brush roll. An inter-roller air passageway may be defined between the leading roller and the brush roll wherein the lower portion of the leading roller is exposed to a flow path to the suction conduit and an upper portion of the leading roller is outside of the flow path. Optionally, a combing unit includes a plurality of combing protrusions extending into the leading roller and having leading edges not aligned with a center of the leading roller. Optionally, a sealing strip is located along a rear side of the opening and along a portion of left and right sides of the opening. The underside may define side edge vacuum passageways extending from the sides of the housing partially between the leading roller and the sealing strip towards the opening.

Abstract: A vehicle wheel alignment system has a plurality of cameras, each camera for viewing a respective target disposed at a respective wheel of the vehicle and capturing image data of the target as the wheel and target are continuously rotated a number of degrees of rotation without a pause. The image data is used to calculate a minimum number of poses of the target of at least one pose for every five degrees of rotation as the wheel and target are continuously rotated the number of degrees of rotation without a pause. At least one of the cameras comprises a data processor for performing the steps of preprocessing the image data, and calculating an alignment parameter for the vehicle based on the preprocessed image data.

Abstract: As demonstrated herein, when composite transcription factor binding sites do not function synergistically, mammalian promoters can be constructed according to simple design rules. Host-cell transcriptional machinery components were analyzed in silico to identify transcription factors with desired expression dynamics. Cognate binding sites were then comprehensively tested in homotypic and heterotypic architectures to assess modularity and determine the transcriptional activity exhibited by a single copy of each site. When elements were specifically selected to prevent combinatorial interactions, heterotypic promoter activities could be accurately modeled simply as a function of constituent binding site copy numbers. As binding site order, spacing, and orientation had minimal effect on promoter activity, blocks could be optimally combined and arranged in silico according to context-specific design-criteria.

Abstract: Simplified models of gene regulation are a fundamental requirement for mammalian synthetic biology. However, at the transcriptional level this has been impeded by the complex design rules governing promoter activity dynamics, preventing de novo-design of regulatory elements with user-defined functionalities. As demonstrated herein, when composite transcription factor binding sites do not function synergistically, mammalian promoters can be constructed according to simple design rules. Host-cell transcriptional machinery components were analyzed in silico to identify transcription factors with desired expression dynamics. Cognate binding sites were then comprehensively tested in homotypic and heterotypic architectures to assess modularity and determine the transcriptional activity exhibited by a single copy of each site.