Abstract: Universal human induced pluripotent stem cells (universal hiPSCs) and a method of forming the same are provided in the disclosure, including the following steps: providing a first cell group including human stem cells; providing a second cell group including human mononuclear cells; in some embodiments, the second cell group further includes human stem cells, in which the human stem cells of the second cell group are allogenic cells from the first cell group; mixing the first cell group and the second cell group to form cell mixture; maintaining the cell mixture at a temperature below 30° C. for at least one day; reprogramming the human stem cells of the cell mixture to obtain universal hiPSCs. The universal hiPSCs includes human leukocyte antigen-1 (HLA class I) gene and human leukocyte antigen-2 (HLA class II) gene, but no HLA class I and HLA class II expressions.

Abstract: The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.

Abstract: The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.

Abstract: The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.

Abstract: A cell culture device includes a container having a surface and a plurality of nano-bristles immobilized to the surface. The nano-bristles are made of styrene copolymer or oligopeptide. A method of partially detaching stem cells from a cell culture device includes: seeding stem cells onto the plurality of nano-bristles, culturing the stem cells, detaching a portion of the stem cells from the nano-bristles by a change in temperature or a shear force, and harvesting the portion of the stem cells.

Abstract: A porous structure for purifying and culturing stem cells includes a substrate. The substrate has a plurality of pores. A pore diameter of the substrate ranges between 8 to 50 ?m, and a material of the substrate is selected from silk, modified fiber, polyester fiber, polyurethane fiber and the combination thereof.

Abstract: The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.

Abstract: The present invention discloses a method for forming a low biofouling filtration membrane. First, an ozone treatment is performed to a fluorine-based porous membrane to introduce peroxides on surface. Afterwards, a first grafting polymerization is initiated from the peroxides, and functional monomers are polymerized to introduce halide groups on surface. Finally, a second grafting polymerization is initiated from the halide groups, and macro-monomers are polymerized to introduce zwitterionic group on surface, so as to form the low biofouling filtration membrane.

Abstract: Disclosed herein are methods and systems for isolating, ex vivo expanding and harvesting hematopoietic stem cells. Methods and systems described herein are easy to use, time-efficient, and allow isolation, ex vivo expansion and harvest of hematopoietic stem cells either batchly or continuously.

Abstract: The present invention discloses a method for forming a low biofouling filtration membrane. First, an ozone treatment is performed to a fluorine-based porous membrane to introduce peroxides on surface. Afterwards, a first grafting polymerization is initiated from the peroxides, and functional monomers are polymerized to introduce halide groups on surface. Finally, a second grafting polymerization is initiated from the halide groups, and macro-monomers are polymerized to introduce zwitterionic group on surface, so as to form the low biofouling filtration membrane.

Abstract: This invention is to provide a catalyst (an artificial enzyme) which can be used as an alternative to a protein enzyme in the field relating to medicine, pharmaceuticals, biochemistry or chemical engineering. Such a catalyst comprises a complex of a transition metal and a monomeric or polymeric nucleotide or an analogue thereof.

Abstract: A membrane for the separation of an organic solvent from a water-organic solvent mixture has a polymeric membrane of a copolymer consisting essentially of an .alpha., .beta.-unsaturated carbonyl compound as a main monomer. The .alpha., .beta.-unsaturated carbonyl compound includes an acrylic acid ester or a methacrylic acid ester. The polymeric membrane has a crosslinked structure. The polymeric membrane is carried on a support member in the form of a flat membrane, a pipe or a hollow fiber. The polymeric member includes particles therein. The polymeric membrane is sandwiched by the support members to form a composite-type separation membrane. The composite-type separation membrane is modulated.

Abstract: A surface-hydrophilic, highly selective semipermeable membrane comprising a semipermeable membrane of a hydrophobic polymer and a hydrophilic segment having at least one end directly bonded to at least one surface of the semipermeable membrane is disclosed. The hydrophilic segment comprises at least one methylene group or substituted methylene group which is positioned at least at one end of the segment and at least one neutral hydroxyl group. The surface-hydrophilic, highly selective semipermeable membrane not only has excellent resistance to heat and organic solvents, but also non-adsorptivity for organic substances including even ionic organic substances. Therefore, the surface-hydrophilic, highly selective semipermeable membrane of the present invention can advantageously be used for microfiltration, ultrafiltration, reverse osmosis and dialysis.