Patents by Inventor Alexander N. Glazer

Alexander N. Glazer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7179618
    Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.
    Type: Grant
    Filed: November 1, 2002
    Date of Patent: February 20, 2007
    Assignee: Regents of the University of California
    Inventors: Takeshi Sano, Alexander N. Glazer, Charles R. Cantor
  • Patent number: 7176000
    Abstract: The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein. including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
    Type: Grant
    Filed: July 10, 2003
    Date of Patent: February 13, 2007
    Assignee: Regents of the University of California
    Inventors: Alexander N. Glazer, Yuping Cai
  • Patent number: 7169582
    Abstract: The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
    Type: Grant
    Filed: July 10, 2003
    Date of Patent: January 30, 2007
    Assignee: Regents of the University of California
    Inventors: Alexander N. Glazer, Yuping Cai
  • Patent number: 6740507
    Abstract: Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
    Type: Grant
    Filed: July 31, 2001
    Date of Patent: May 25, 2004
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Aaron J. Tooley, Yuping Cai
  • Publication number: 20040014151
    Abstract: The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein. including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
    Type: Application
    Filed: July 10, 2003
    Publication date: January 22, 2004
    Applicant: The Regents of the University of California
    Inventors: Alexander N. Glazer, Yuping Cai
  • Publication number: 20040009559
    Abstract: The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
    Type: Application
    Filed: July 10, 2003
    Publication date: January 15, 2004
    Applicant: The Regents of the University of California
    Inventors: Alexander N. Glazer, Yuping Cai
  • Publication number: 20030215922
    Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.
    Type: Application
    Filed: November 1, 2002
    Publication date: November 20, 2003
    Inventors: Takeshi Sano, Alexander N. Glazer, Charles R. Cantor
  • Patent number: 6649376
    Abstract: The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
    Type: Grant
    Filed: December 21, 1999
    Date of Patent: November 18, 2003
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Yuping Cai
  • Patent number: 6573047
    Abstract: The present invention provides methods and kits for identifying nucleotides at a variant site in a target molecule by forming fluorescence resonance energy transfer labeled product. The location and type of fluorescent labels in the labeled product provide strong signals and relatively high spectral purity that facilitate detection. Utilizing various secondary labels and different combinations of acceptor and donor labels, certain methods permit multiple analyses to be conducted simultaneously and at high throughput. The methods can be used in a variety of applications such as analyzing point mutations and single nucleotide polymorphisms (SNPs). In addition, the methods have utility in other applications in which specific sequence information is of value, including detection of pathogens, paternity disputes, prenatal testing and forensic analysis.
    Type: Grant
    Filed: April 11, 2000
    Date of Patent: June 3, 2003
    Assignee: DNA Sciences, Inc.
    Inventors: Su-Chun Hung, Alexander N. Glazer, Richard A. Mathies
  • Publication number: 20030027285
    Abstract: Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
    Type: Application
    Filed: July 31, 2001
    Publication date: February 6, 2003
    Inventors: Alexander N. Glazer, Aaron J. Tooley, Yuping Cai
  • Patent number: 6428667
    Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
    Type: Grant
    Filed: October 10, 2000
    Date of Patent: August 6, 2002
    Assignee: The Regents of the University of California, Berkeley
    Inventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
  • Patent number: 6391590
    Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.
    Type: Grant
    Filed: October 21, 1991
    Date of Patent: May 21, 2002
    Assignee: The Regents of the University of California
    Inventors: Takeshi Sano, Alexander N. Glazer, Charles R. Cantor
  • Patent number: 6361671
    Abstract: This invention relates to a microfabricated capillary electrophoresis chip for detecting multiple redox-active labels simultaneously using a matrix coding scheme and to a method of selectively labeling analytes for simultaneous electrochemical detection of multiple label-analyte conjugates after electrophoretic or chromatographic separation.
    Type: Grant
    Filed: January 11, 1999
    Date of Patent: March 26, 2002
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Pankaj Singhal, Jin Xie, Alexander N. Glazer
  • Patent number: 6355433
    Abstract: The present invention provides methods and kits for determining the identity of a nucleotide at a variant site in a target nucleic acid of interest, including, for example, point mutations and single nucleotide polymorphisms. The methods involve conducting template-dependent extension reactions in the presence of a mixture of nucleotides that include at least one labeled extendible nucleotide and at least one labeled non-extendible nucleotide that are selected to be complementary to the nucleotides that potentially occupy the variant site. The particular labeled nucleotide incorporated into the extension products is characteristic of the nucleotide at the variant site.
    Type: Grant
    Filed: June 2, 2000
    Date of Patent: March 12, 2002
    Assignee: DNA Sciences, Inc.
    Inventors: Hua Xu, Alexander N. Glazer
  • Patent number: 6280933
    Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
    Type: Grant
    Filed: November 7, 1997
    Date of Patent: August 28, 2001
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
  • Patent number: 6150107
    Abstract: Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
    Type: Grant
    Filed: October 1, 1998
    Date of Patent: November 21, 2000
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Richard A. Mathies, Su-Chun Hung, Jingyue Ju
  • Patent number: 6054272
    Abstract: Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
    Type: Grant
    Filed: November 7, 1997
    Date of Patent: April 25, 2000
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Scott C. Benson
  • Patent number: 6045676
    Abstract: A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.
    Type: Grant
    Filed: August 22, 1997
    Date of Patent: April 4, 2000
    Assignee: The Board of Regents of the University of California
    Inventors: Richard A. Mathies, Alexander N. Glazer, Adam T. Woolley, Kaigin Lao
  • Patent number: 5962223
    Abstract: The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed.
    Type: Grant
    Filed: December 19, 1995
    Date of Patent: October 5, 1999
    Assignee: The Perkin-Elmer Corporation
    Inventors: Norman M. Whiteley, Michael W. Hunkapiller, Alexander N. Glazer
  • Patent number: 5929227
    Abstract: Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.
    Type: Grant
    Filed: November 7, 1997
    Date of Patent: July 27, 1999
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Scott C. Benson