Patents by Inventor Alfred Puehler

Alfred Puehler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6815196
    Abstract: The present invention relates to isolated polynucleotides from Corynebacterium glutamicum encoding proteins of the transcription activator (MetR) and O-succinylhomoserine sulfhydrylase (MetZ). The invention also relates to producing L-amino acids, particularly methionine, in coryneform cells having attenuated metR and metZ genes.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: November 9, 2004
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Patent number: 6812016
    Abstract: The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: November 2, 2004
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Patent number: 6740742
    Abstract: The invention relates to an isolated polynucleotide from coryneform bacteria wherein the polynucleotide encodes a protein having n-succinylaminokeptopimelate transaminase activity.
    Type: Grant
    Filed: March 22, 2001
    Date of Patent: May 25, 2004
    Assignee: Degussa AG
    Inventors: Bettina Mockel, Anke Weissenborn, Walter Pfefferle, Michael Hartmann, Jörn Kalinowski, Alfred Pühler
  • Patent number: 6713289
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Grant
    Filed: May 21, 2001
    Date of Patent: March 30, 2004
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Thomas Hermann, Alfred Pühler, Jörn Kalinowski, Brigitte Bathe
  • Patent number: 6670156
    Abstract: The present application is directed to a diaminopimelate epimerase from Corynebacterium glutamicum and to polynucleotides encoding this enzyme. The gene has been given the designation “dapF.
    Type: Grant
    Filed: October 27, 1999
    Date of Patent: December 30, 2003
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Brigitte Bathe, Jorn Kalinowski, Oliver Kirchner, Michael Hartmann, Alfred Pühler
  • Patent number: 6632644
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Grant
    Filed: December 8, 2000
    Date of Patent: October 14, 2003
    Assignee: DeGussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
  • Publication number: 20030092026
    Abstract: The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the metD gene and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of MetD transcription regulator activity, methods for preparation of L-amino acids, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of MetD transcription regulator activity. Further, the invention relates to animal food additives based on fermentation liquor and containing L-methionine, and to the preparation of such additive.
    Type: Application
    Filed: May 30, 2002
    Publication date: May 15, 2003
    Applicant: DEGUSSA AG
    Inventors: Daniel Rey, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Brigitte Bathe, Klaus Huthmacher, Walter Pfefferle
  • Patent number: 6555733
    Abstract: The invention relates to DNA molecules which code for deacetylases or proteins having the biological activity of a deacetylase and also transgenic plant cells which were transformed using the DNA molecules according to the invention. These molecules can be used for the production of plants having specifically destroyable parts, i.e. male- or female-sterile plants, by means of the specific expression of a deacetylase gene.
    Type: Grant
    Filed: November 30, 2000
    Date of Patent: April 29, 2003
    Assignee: Hoechst Schering AgrEvo GmbH
    Inventors: Klaus Bartsch, Guido Kriete, Inge Broer, Alfred Pühler
  • Publication number: 20020110877
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of
    Type: Application
    Filed: August 2, 2001
    Publication date: August 15, 2002
    Applicant: DEGUSSA AG
    Inventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20020110878
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of
    Type: Application
    Filed: August 2, 2001
    Publication date: August 15, 2002
    Applicant: DEGUSSA AG
    Inventors: Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20020102664
    Abstract: Polynucleotides from coryneform bacteria which code for the metR and/or metZ genes and comprise polynucleotide sequences, selected from the group consisting of
    Type: Application
    Filed: August 2, 2001
    Publication date: August 1, 2002
    Applicant: DEGUSSA AG
    Inventors: Brigitte Bathe, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20020048793
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of
    Type: Application
    Filed: August 2, 2001
    Publication date: April 25, 2002
    Applicant: DEGUSSA AG
    Inventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Patent number: 5633154
    Abstract: A method of locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:(1) The construction of a non-self-transferrable vector mobilizable from an E. coli mobilizer strain which vector is composed of(a) A DNA segment containing a replicon functional in E. coli,(b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT),(c) A third DNA segment which recombines homologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria,(d) A DNA segment from Bacillus subtilis containing the sacB gene,(2) Transfer of this vector by means of conjugative transfer into the coryneform recipient strains,(3) Cultivation of the transconjugants containing the vector in an .about.
    Type: Grant
    Filed: November 4, 1994
    Date of Patent: May 27, 1997
    Assignee: Degussa Aktiengesellschaft
    Inventors: Andreas Schaefer, Anna-Hildegard Seep-Feldhaus, Wolfgang Jaeger, Joern Kalinowski, Wolfgang Wohlleben, Alfred Puehler
  • Patent number: 5380657
    Abstract: A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:(1) The construction of a non-self-transferrable vector mobilizable from an E. coli mobilizer strain which vector is composed of(a) A DNA segment containing a replicon functional in E. coli,(b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT),(c) A third DNA segment which recombines homologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria,(d) A DNA segment from Bacillus subtilis containing the sacB gens,(2) Transfer of this vector by means of conjugative transfer into the coryneform recipient strains,(3) Cultivation of the transconjugants containing the vector in an .about.
    Type: Grant
    Filed: March 18, 1993
    Date of Patent: January 10, 1995
    Assignee: Degussa Aktiengesellschaft
    Inventors: Andreas Schaefer, Anna-Hildegard Seep-Feldhaus, Wolfgang Jaeger, Joern Kalinowski, Wolfgang Wohlleben, Alfred Puehler