Abstract: A vehicle comprises a front longitudinal beam, a subframe and a subframe front mounting point, wherein the subframe front mounting point is connected to the front longitudinal beam, the subframe front mounting point is arranged on the outside of the front longitudinal beam in the widthwise direction of the vehicle, and the subframe is connected to the front longitudinal beam by means of the subframe front mounting point.

Abstract: A safety belt lower anchorage front-back adjustment structure may have a bottom base and a seat frame that is mounted on the bottom base. A support base may be respectively mounted on the bottom base at positions corresponding to a left side and a right side of the seat frame. A sliding component may be provided on each the support base. Two of the sliding components may be able to slide forward and backward on the corresponding support base. A driving assembly may be provided between the sliding component and the corresponding support base used for driving the sliding component to slide forward and backward among the two sliding components. A buckle may be fixedly mounted on one sliding component, and a lower anchor is fixedly mounted on the other sliding component.

Abstract: Provided are a human LIFR antigen binding protein, a preparation method therefor, and an application thereof. A series of human LIFR antibodies having biological functions are obtained using human LIFR-ECD protein immune H2L2 humanized antibody transgenic mice by means of single B cell cloning technology (SBC), single B cell sequencing analysis, and Fc recombination. The method greatly improves the discovery efficiency and the yield of antibody drugs, and the obtained human LIFR antibody has good affinity and can block the binding of huLIF proteins to huLIFR/gp130 cells, thereby inhibiting signaling pathways such as STAT3, further inhibiting the growth of tumors, and achieving the purpose of tumor prevention and treatment.

Abstract: Disclosed are a thermal management system and a vehicle. The thermal management system includes a refrigerant circuit and a water circuit. The refrigerant circuit is provided with a liquid refrigerant and includes a corresponding circuit formed through a plurality of components among a compressor, a first heat exchanger, a second heat exchanger, a condenser, a first evaporator, a second evaporator, a first expansion valve, a second expansion valve, and a third expansion valve according to needs. The water circuit is provided with a cooling liquid and includes a corresponding circuit formed through a plurality of components among the first heat exchanger, a first water pump, a drive assembly, a first four-way valve, a second four-way valve, a battery assembly, a second water pump, a three-way valve, and the second heat exchanger according to needs.

Abstract: A method is provided for more effectively agitating a vibration responsive liquid in a container to enhance the qualities of the liquid. Vibration energy that is within the audio frequency spectrum and that corresponds to one or more resonant frequencies of the liquid filled container is coupled to the wall of the container. One or more vibration responsive sensors deployed in or on a container containing the vibration responsive liquid are used to determine the container's resonant frequencies. The method is particularly used in the aging of alcoholic beverages.

Abstract: Disclosed are a battery frame, a power battery and a vehicle. The battery frame includes a base plate and side beams. The side beams are provided on opposite sides of the base plate, each side beam includes a side beam part, a connecting part provided inside the side beam part and connected to the base plate, and a fixing part provided outside the side beam part and configured to connect to a door sill beam of a vehicle. The side beam part includes a upper side beam and a lower side beam, the upper side beam is provided above the lower side beam, an outer side of the lower side beam is connected to the fixing part, and an inner side of the lower side beam is connected to the connecting part, and a first cavity is provided at the lower side beam.

Abstract: Described herein are PKMYT1 (Myt1) inhibitors and pharmaceutical compositions comprising said inhibitors. The subject compounds and compositions are useful for the treatment of a disease or disorder associated with PKMYT1.

Abstract: A bike trailer frame structure includes a rear frame and a base frame. The rear side of the base frame is mounted with left and right pivot bases, that have a fixing hole (fixing groove) respectively connected to the bottom side of the rear frame through a respective positioning element. Thereby, when the right positioning elements are released, the rear frame can pivot toward the base frame and fold, or can pivot in the direction opposite to the base frame, and then fix the positioning elements to the fixing holes or fixing grooves of the pivot bases to complete the unfolding action. Therefore, the invention simplifies the folding structure of the rear frame and the base frame in the front and rear directions. It has the practicality of streamlined components, uncomplicated manufacturing, simple and convenient assembly, and saving transportation space.

Abstract: Compounds having activity as inhibitors of G12C mutant KRAS protein are provided. The compounds have the following structure (I): or a pharmaceutically acceptable salt, stereoisomer, isotopic form or prodrug thereof, wherein R1, L1, L2, L3, A1, A2, A3, A4, G1, G2, E, W, X, Y, Z, m, and n are as defined herein. Methods associated with preparation and use of such compounds, pharmaceutical compositions comprising such compounds and methods to modulate the activity of G12C mutant KRAS protein for treatment of disorders, such as cancer, are also provided.

Abstract: A silicone-vinylester copolymer and methods for its preparation and use are provided. The silicone-vinylester copolymer can be made using a vinylester-functional siloxane macromonomer, which can be prepared by a process comprising hydroformylation of an alkenyl-functional polyorganosiloxane to form an aldehyde-functional polyorganosiloxane; oxidation of the aldehyde-functional polyorganosiloxane to form a carboxy-functional polyorganosiloxane, and transvinylation of the carboxy-functional polyorganosiloxane to form the vinylester-functional siloxane macromonomer. The silicone-vinylester copolymer is useful in personal care compositions.

Abstract: The present invention belongs to the technical field of characterization of target analyte properties, and particularly provides a mutant of a porin monomer, a protein pore comprising same, and use thereof in the detection of a target analyte, wherein an amino acid of the mutant of the porin monomer comprises mutations at one or more of positions corresponding to A74, P75, G76, N77, A78, T79, N80, and F81 of SEQ ID NO: 1.

Abstract: Disclosed is a method for recycling a polyester blend fabric. The method includes: treating a polyester-containing raw material to be treated, subjecting a resulting treated polyester-containing raw material to polyester degradation at 40° C. to 120° C. for 0.5 h to 8 h in the presence of a catalyst to achieve complete depolymerization, separating out and recovering a polyester component, and reutilizing the polyester component and other blend components.

Abstract: An anti-oxidation dipping treatment method for a graphite sealing element for a thermal power generation unit, and an anti-oxidation production line. The anti-oxidation dipping treatment method for a graphite sealing element for a thermal power generation unit comprises the following steps: S1, placing a graphite sheet into a soaking device for soaking; S2, conveying the soaked graphite sheet into a drying and curing device for drying and curing; S3, stacking the multiple dried and cured graphite sheets together, and subjecting same to compression molding to form a layered graphite body; and S4, stamping the layered graphite body to form a finished graphite sealing element with a desired appearance.

Abstract: Disclosed are a drug delivery carrier complex targeting a central nervous system and a preparation method and an application thereof, relating to the technical field of biomedicine. The preparation method includes following steps: carrying out azide modification on carrier cells to obtain azide-modified carrier cells; modifying sulfhydryl group and cholesterol to two ends of therapeutic miRNA respectively, and then performing ligation reaction to modified therapeutic miRNA and an acryl complex to form a miRNA complex; and co-incubating the azide-modified carrier cells with the miRNA complex, and forming the drug delivery carrier complex through click chemistry reaction.

Abstract: Disclosed are a method for extracting lithium from brine, and an application. The method includes performing extraction on the brine through a continuous ion exchange process, where according to the continuous ion exchange process, in an adsorption section, adsorption is performed on the brine with an aluminum-based lithium adsorbent; in a water-pushing-material section, a resin column is washed with a solution; in a desorption section, desorption is performed with pure water, and a desorption solution is collected in segments; and in a material-pushing-water section, a solution in a resin column is replaced by spent brine.

Abstract: A transcription regulation system based on CRISPRi and CRISPRa, and an establishment method therefor and the use thereof. The system is a new transcription regulation system which has high expression intensity, a low leakage level and is flexible and programmable. Provided is a method for achieving high-intensity and low-leakage expression of a gene by means of the transcription regulation system, wherein a high-intensity transcription level is achieved while suppressing background expression by means of synergistic regulation on downstream signaling effect devices by means of CRISPRi and CRISPRa dev ices. The new transcription regulation system can obtain, by means of loading different input promoters, a new expression system for responding to a specific signal, and has application value in the development and establishment of an efficient heterologous protein expression platform and a microbial cell factory.

Abstract: The invention pertains to means and methods for fluorescent in situ hybridization (FISH) using a CRISPR CAS9 variant with nickase activity in order to denature the DNA double strand and then using an exonuclease in order to remove the DNA strand that is not targeted with the FISH probes. The invention enhances FISH signal to noise ratio and allows a highly specific targeting of genomic locations via the used sgRNA and FISH probe sequences. In addition, the invention provides uses and kits for the enhanced FISH methods.

Abstract: Described herein are compositions, vectors, cells, methods, and kits that provide cell data recorder systems for recording cell states. The cell data recorder systems allow for the recording of both the presence and duration of one or more stimuli in a programmable, reproducible, and multiplexable manner. These cell data recorder systems employ a nucleic acid programmable DNA binding protein, such as a Cas9 nuclease, or a fusion protein comprising a nucleic acid programmable DNA binding domain and a nucleic acid editing domain to introduce recordable changes in the genome of a cell or in a plasmid within the cell.

Abstract: The present invention discloses a set of SNP markers related to heat tolerance of Pisum sativum developed based on a SnaPshot technology, and use thereof. In the present invention, 2,358 accessions of Pisum sativum germplasms from all over the world are subjected to a heat tolerance screening experiment by employing a manner of sowing by stages, and a reasonable Pisum sativum heat-tolerant classifying standard is established through identification of yield-related traits, so that a Pisum sativum germplasm population including heat-tolerant and heat-sensitive germplasms is obtained; and the population is subjected to genetic diversity and population genetic structure analysis through a set of SNP markers (20 markers) related to heat tolerance of Pisum sativum developed based on an SNaPshot technology, so as to provide theoretical and practical basis for the future study of heat-tolerant genetic mechanism and heat-tolerant breeding of Pisum sativum.

Abstract: Provided herein are methods and systems for cell-free DNA extraction from liquid biological samples. The methods can be employed for determination of fetal DNA fraction and non- invasive prenatal screening of fetal aneuploidies and analyses of other types of cell-free DNA.