Patents by Inventor Anastassios Giannakopulos

Anastassios Giannakopulos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200328068
    Abstract: A data independent acquisition method of mass spectrometry for analyzing a sample within a mass range of interest as it elutes from a chromatography system. The method comprises selecting precursor ions within a mass range of interest to be analyzed, performing at least one MS1 scan of the precursor ions using a first, high-resolution mass analyzer and performing a set of MS2 scans by segmenting the precursor ions into a plurality of precursor mass segments, each precursor mass segment having a mass range of no greater than 5 amu, and for each precursor mass segment fragmenting the precursor ions within that precursor mass segment and performing an MS2 scan of the fragmented ions using a time of flight mass analyzer.
    Type: Application
    Filed: June 26, 2020
    Publication date: October 15, 2020
    Inventors: Anastassios GIANNAKOPULOS, Alexander MAKAROV, Christian Albrecht HOCK, Nicolaie Eugen DAMOC, Hamish STEWART
  • Publication number: 20200258727
    Abstract: Calibration of a mass spectrometer is described. In one aspect, a mass spectrometer can generate an offset value indicative of the mass difference between the corrected and reference external calibrant ion data. By comparing the offset value to a threshold, a preliminary mass calibration can be modified, or a recalibration of the mass spectrometer is performed.
    Type: Application
    Filed: January 22, 2020
    Publication date: August 13, 2020
    Inventor: Anastassios Giannakopulos
  • Patent number: 10699888
    Abstract: A data independent acquisition method of mass spectrometry for analyzing a sample within a mass range of interest as it elutes from a chromatography system. The method comprises selecting precursor ions within a mass range of interest to be analyzed, performing at least one MS1 scan of the precursor ions using a Fourier Transform mass analyser and performing a set of MS2 scans by segmenting the precursor ions into a plurality of precursor mass segments, each precursor mass segment having a mass range of no greater than 5 amu, and for each precursor mass segment fragmenting the precursor ions within that precursor mass segment and performing an MS2 scan of the fragmented ions.
    Type: Grant
    Filed: May 30, 2018
    Date of Patent: June 30, 2020
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Anastassios Giannakopulos, Alexander Makarov, Christian Albrecht Hock, Nicolaie Eugen Damoc, Hamish Stewart
  • Publication number: 20180350576
    Abstract: A data independent acquisition method of mass spectrometry for analyzing a sample within a mass range of interest as it elutes from a chromatography system. The method comprises selecting precursor ions within a mass range of interest to be analyzed, performing at least one MS1 scan of the precursor ions using a Fourier Transform mass analyser and performing a set of MS2 scans by segmenting the precursor ions into a plurality of precursor mass segments, each precursor mass segment having a mass range of no greater than 5 amu, and for each precursor mass segment fragmenting the precursor ions within that precursor mass segment and performing an MS2 scan of the fragmented ions.
    Type: Application
    Filed: May 30, 2018
    Publication date: December 6, 2018
    Inventors: Anastassios GIANNAKOPULOS, Alexander MAKAROV, Christian Albrecht HOCK, Nicolaie Eugen DAMOC, Hamish STEWART
  • Patent number: 10074528
    Abstract: The invention provides a data acquisition system and method for detecting ions in a mass spectrometer, comprising: a detection system for detecting ions comprising two or more detectors for outputting two or more detection signals in separate channels in response to ions arriving at the detection system; and a data processing system for receiving and processing the detection signals in separate channels of the data processing system and for merging the processed detection signals to construct a mass spectrum; wherein the processing in separate channels comprises removing noise from the detection signals by applying a threshold to the detection signals. The detection signals are preferably produced in response to the same ions, the signals being shifted in time relative to each other. The invention is suitable for a TOF mass spectrometer.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: September 11, 2018
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Alexander A. Makarov, Anastassios Giannakopulos, Matthias Biel
  • Patent number: 9947525
    Abstract: A technique for time interval measurement is provided. First and second signal components are received, sampled and digitized. The first signal component is derived from a trigger signal that causes or indicates generation 5 of the second signal component. A time interval between the first and second signal components is determined based on a reference time defined by the sampled and digitized first signal component and based on a reference time defined by the sampled and digitized second signal component.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: April 17, 2018
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Matthias Biel, Richard Heming, Anastassios Giannakopulos
  • Publication number: 20160336162
    Abstract: A technique for time interval measurement is provided. First and second signal components are received, sampled and digitized. The first signal component is derived from a trigger signal that causes or indicates generation 5 of the second signal component. A time interval between the first and second signal components is determined based on a reference time defined by the sampled and digitized first signal component and based on a reference time defined by the sampled and digitized second signal component.
    Type: Application
    Filed: May 10, 2016
    Publication date: November 17, 2016
    Inventors: Matthias BIEL, Richard HEMING, Anastassios GIANNAKOPULOS
  • Patent number: 9412578
    Abstract: A method of separating charged particles using an analyzer is provided, the method comprising: causing a beam of charged particles to fly through the analyzer and undergo within the analyzer at least one full oscillation in the direction of an analyzer axis (z) of the analyzer whilst orbiting about the axis (z) along a main flight path; constraining the arcuate divergence of the beam as it flies through the analyzer; and separating the charged particles according to their flight time. An analyzer for performing the method is also provided. At least one arcuate focusing lens is preferably used to constrain the divergence, which may comprise a pair of opposed electrodes located either side of the beam.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: August 9, 2016
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Alexander A. Makarov, Anastassios Giannakopulos
  • Patent number: 9099287
    Abstract: A multi reflection time of flight (MRTOF) mass spectrometer (12) And method for identifying a sample is disclosed. Sample ions are generated at an ion source (15). The MRTOF is a closed mirror arrangement with first and second opposed ion mirrors (20, 20?) on an axis of reflection (XX?). The MRTOF (12) also includes a bidirectional ion deflector (50) on that axis (XX?). The deflector (50) deflects ions onto the reflection axis as a short pulse at time to <zero> where they oscillate multiple times, separating in time of flight according to ion m/z. At a later time t, ions travelling in both directions along the axis (XX?) are ejected out of the MRTOF (12) by the bidirectional deflector (50) to an ion detector arrangement (55). The separation of ions in time of flight allows a “fingerprint” of a biological sample to be produced by the detector arrangement (55) without the need to assign a mass to each peak. Comparison with a library of fingerprints permits identification.
    Type: Grant
    Filed: June 19, 2012
    Date of Patent: August 4, 2015
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventor: Anastassios Giannakopulos
  • Patent number: 8952322
    Abstract: A method and apparatus for high resolution separation of ions based on their high field and low-field mobility properties is described. An elongate ion separation channel is defined by a plurality of channel walls. First and second channel walls have first and second spaced ion separation electrode assemblies respectively. A power supply applies a periodic asymmetric potential to one or both of the electrode assemblies so as to generate a periodically asymmetric electric field in the channel for ion mobility separation. The channel walls define an ion separation channel of substantially helical shape.
    Type: Grant
    Filed: September 8, 2011
    Date of Patent: February 10, 2015
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventor: Anastassios Giannakopulos
  • Patent number: 8916819
    Abstract: A method of increasing ion throughput within an accumulator, an energy lift and a pulsed ion extractor, operated in that order upon a batch of ions, comprising the steps of: firstly loading a batch of ions into the accumulator; secondly changing the electrical potential of the energy lift to raise the energy of the batch of ions contained therein; and thirdly ejecting the batch of ions from the pulsed ion extractor; and wherein: the energy lift is a separate device from the accumulator and the pulsed ion extractor, and whilst changing the electrical potential in the second step a fresh batch of ions is loaded into the accumulator and/or a previous batch of ions is prepared for ejection in the pulsed ion extractor; or the energy lift is incorporated into the pulsed ion extractor and whilst changing the electrical potential in the second step a fresh batch of ions is loaded into the accumulator; or the energy lift is incorporated into the accumulator and whilst changing the electrical potential in the second st
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: December 23, 2014
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Alexander Makarov, Anastassios Giannakopulos
  • Publication number: 20140175274
    Abstract: Methods and analysers useful for time of flight mass spectrometry are provided.
    Type: Application
    Filed: January 17, 2014
    Publication date: June 26, 2014
    Applicant: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Alexander A. Makarov, Anastassios Giannakopulos
  • Patent number: 8759751
    Abstract: Methods and analyzers useful for time of flight mass spectrometry are provided.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: June 24, 2014
    Assignee: Thermo Fisher Scientific (Brmen) GmbH
    Inventors: Alexander Makarov, Anastassios Giannakopulos
  • Publication number: 20140166876
    Abstract: A method of separating charged particles using an analyser is provided, the method comprising: causing a beam of charged particles to fly through the analyser and undergo within the analyser at least one full oscillation in the direction of an analyser axis (z) of the analyser whilst orbiting about the axis (z) along a main flight path; constraining the arcuate divergence of the beam as it flies through the analyser; and separating the charged particles according to their flight time. An analyser for performing the method is also provided. At least one arcuate focusing lens is preferably used to constrain the divergence, which may comprise a pair of opposed electrodes located either side of the beam.
    Type: Application
    Filed: January 31, 2014
    Publication date: June 19, 2014
    Applicant: THERMO FISHER SCIENTIFIC (BREMEN) GMBH
    Inventors: Alexander A. Makarov, Anastassios Giannakopulos
  • Publication number: 20140117226
    Abstract: A multi reflection time of flight (MRTOF) mass spectrometer (12) And method for identifying a sample is disclosed. Sample ions are generated at an ion source (15). The MRTOF is a closed mirror arrangement with first and second opposed ion mirrors (20, 20?) on an axis of reflection (XX?). The MRTOF (12) also includes a bidirectional ion deflector (50) on that axis (XX?). The deflector (50) deflects ions onto the reflection axis as a short pulse at time to <zero> where they oscillate multiple times, separating in time of flight according to ion m/z. At a later time t, ions travelling in both directions along the axis (XX?) are ejected out of the MRTOF (12) by the bidirectional deflector (50) to an ion detector arrangement (55). The separation of ions in time of flight allows a “fingerprint” of a biological sample to be produced by the detector arrangement (55) without the need to assign a mass to each peak. Comparison with a library of fingerprints permits identification.
    Type: Application
    Filed: June 19, 2012
    Publication date: May 1, 2014
    Inventor: Anastassios Giannakopulos
  • Patent number: 8680481
    Abstract: Embodiments of the invention provide a detection apparatus for detecting charged particles having a secondary particle generator for generating secondary charged particles in response to receiving incoming charged particles, a charged particle detector for receiving and detecting secondary charged particles generated by the secondary particle generator, a photon generator for generating photons in response to receiving secondary charged particles generated by the secondary particle generator, and a photon detector for detecting the photons generated by the photon generator.
    Type: Grant
    Filed: October 21, 2010
    Date of Patent: March 25, 2014
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Anastassios Giannakopulos, Alexander A. Makarov
  • Patent number: 8658984
    Abstract: A method of separating charged particles using an analyzer is provided, the method comprising: causing a beam of charged particles to fly through the analyzer and undergo within the analyzer at least one full oscillation in the direction of an analyzer axis (z) of the analyzer whilst orbiting about the axis (z) along a main flight path; constraining the arcuate divergence of the beam as it flies through the analyzer; and separating the charged particles according to their flight time. An analyzer for performing the method is also provided. At least one arcuate focusing lens is preferably used to constrain the divergence, which may comprise a pair of opposed electrodes located either side of the beam.
    Type: Grant
    Filed: May 27, 2010
    Date of Patent: February 25, 2014
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Alexander A. Makarov, Anastassios Giannakopulos
  • Patent number: 8642973
    Abstract: Embodiments of the invention provide a detection apparatus for detecting charged particles having a charged particle detector for receiving and detecting either incoming charged particles or secondary charged particles generated from the incoming charged particles, a photon generator for generating photons in response to receiving at least some of the same incoming charged particles or secondary charged particles generated from the incoming charged particles as are received and detected by the charged particle detector, and a photon detector for detecting photons generated by the photon generator.
    Type: Grant
    Filed: October 21, 2010
    Date of Patent: February 4, 2014
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Anastassios Giannakopulos, Alexander A. Makarov
  • Patent number: 8637815
    Abstract: Methods and analysers useful for time of flight mass spectrometry are provided.
    Type: Grant
    Filed: May 27, 2010
    Date of Patent: January 28, 2014
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Alexander A. Makarov, Anastassios Giannakopulos
  • Publication number: 20130268212
    Abstract: The invention provides a data acquisition system and method for detecting ions in a mass spectrometer, comprising: a detection system for detecting ions comprising two or more detectors for outputting two or more detection signals in separate channels in response to ions arriving at the detection system; and a data processing system for receiving and processing the detection signals in separate channels of the data processing system and for merging the processed detection signals to construct a mass spectrum; wherein the processing in separate channels comprises removing noise from the detection signals by applying a threshold to the detection signals. The detection signals are preferably produced in response to the same ions, the signals being shifted in time relative to each other. The invention is suitable for a TOF mass spectrometer.
    Type: Application
    Filed: December 15, 2011
    Publication date: October 10, 2013
    Inventors: Alexander A. Makarov, Anastassios Giannakopulos, Matthias Biel