Abstract: Disclosed herein are compositions and method comprising non-canonical (e.g., non-C2H2) zinc finger proteins.

Abstract: Disclosed herein are compositions and method comprising non-canonical (e.g., non-C2H2) zinc finger proteins.

Abstract: The invention provides a pharmaceutical composition for the transmucosal delivery of a statin, said composition comprising a statin and a carrier in which said statin is soluble or forms a suspension or emulsion. The invention also provides a pharmaceutical composition comprising a statin for use in inhibiting cholesterol synthesis in vivo, where in said use said composition is delivered by the transmucosal route.

Abstract: A method and an apparatus are provided for determining the location of a mobile device using multiple wireless access points, each wireless access point comprising multiple antennas. The apparatus and method are particularly suited to location of a mobile device in an indoor environment, such as a building. The method includes receiving a communication signal from the mobile device at each of multiple antennas of said multiple wireless access points; for each wireless access point, determining angle-of-arrival information of the received communication signal at the wireless access point, based on a difference in phase of the received signal between different antennas; collecting, from each of the multiple wireless access points, the determined angle-of-arrival information for the received communication signal from the mobile device; and estimating the location of the mobile device from the collected angle-of-arrival information.

Abstract: A subsea payload such as an AUV (autonomous underwater vehicle) or an AUV garage is lifted from an underwater location by flying a latch unit through the water to the payload. The latch unit carries a lift line toward the payload. The latch unit is then attached to the payload and the payload is lifted using tension applied through the lift line via the latch unit. The latch unit can also be used on a lift line to lower a payload and then to release the payload at an underwater location. The lift line is supported by a heave-compensating winch on a surface vessel that effects z-axis movement of the latch unit. The winch maintains tension on the lift line to prevent the lift line falling on the payload. Movement of the latch unit on x- and y-axes is effected by on-board thrusters.

Abstract: The invention provides a pharmaceutical composition for the transmucosal delivery of a PDE5 inhibitor, said composition comprising a PDE5 inhibitor and a carrier in which said PDE5 inhibitor is soluble or forms a suspension or emulsion. The invention also provides a pharmaceutical composition comprising a PDE5 inhibitor for use in inhibiting PDE activity in vivo, where in said use said composition is delivered by the transmucosal route.

Abstract: Disclosed herein are modified plant zinc finger proteins; compositions comprising modified plant zinc finger proteins and methods of making and using modified plant zinc finger proteins. The modified plant zinc finger proteins, in contrast to naturally-occurring plant zinc finger proteins, have a binding specificity that is determined by tandem arrays of modular zinc finger binding unit.

Abstract: The present application describes general process for the preparation of amino-substituted gamma-lactams involving the reaction of synthons of the general Formulae (I) and (VI): with amines. The processes are amenable to solid phase synthetic techniques and therefore allow the efficient incorporation of amino-substituted gamma-lactams into a wide variety of structural scaffolds, including, in particular peptides.

Abstract: A method and apparatus of operating an autonomous underwater vehicle (AUV) may include receiving an AUV in a receptacle of a submersible station. The AUV and the submersible station may be launched to an underwater location. The AUV may engage with the submersible station before, after or during a mission, and may return to the same or a different submersible station after part, or all, of the mission has been completed.

Abstract: A system for the authentication by a card-issuing financial institution of identifying information of a card-holding user of a public data network. The system includes a secure data entry device connected to the public data network and a gateway device connected to the public data network and to a private data network used for transmitting messages between financial institutions. The secure data entry device enables the user to enter identifying information of a card issued by the card-issuing financial institution, and transmits the identifying information in a secure manner over the public data network to the gateway device. The gateway device transmits the identifying information to the card-issuing financial institution and receives an approval response from the card-issuing financial institution over the private data network. The approval response provides authentication of the identifying information by the card-issuing financial institution.

Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

Abstract: Disclosed herein are zinc finger proteins that bind to target sites in a plant gamma-tocopherol methyl transferase (GMT) gene; compositions comprising these GMT-targeted zinc finger proteins and methods of making and using such zinc finger proteins. Also disclosed are methods for modulating the alpha-tocopherol content in various plant organs, particularly seeds, in transgenic plants.

Abstract: The present invention relates to methods of using libraries of randomized zinc finger proteins to identify genes associated with selected phenotypes.

Abstract: A display device is disclosed which may be used in a mobile telephone or other article which is intended to make secure transactions such as financial transactions, as well as other personal transactions such as telephone calls. The device includes a display (24) for displaying information, a financial transaction controller (26) including a processor (28), a display driver (30) for driving the display (24) and a secure memory (32). The financial transaction controller allows the display section (24), and an input keypad (12), to operate under the control of the device to enable personal functions other than financial transactions to be performed.

Abstract: The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5?NNGK3?. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.

Abstract: A strain-responsive visual indicator comprises a pair of overlapping shutter strips (1, 2) adapted to be mounted to a substrate such that strain in the substrate causes relative movement M between them. Each shutter strip comprises an alternating set of windows (5) or (6) and bars (7) or (8) and the distal strip (2) is backed by a strip (3) of a different color exposed through its windows (6), or the distal strip can instead be printed with a set of alternating colors in place of its windows and bars. The effect is that under different strain conditions different color indications from the distal strip (2) will be visible through the windows (5) of the proximal strip (1), in accordance with the relative positions of the two strips. The indicator can be incorporated e.g. in the shaft of a toothbrush to give a visual warning in response to flexure of the shaft if excessive brushing pressure is applied. Numerous other applications and the use of different types of indicia are indicated in the specification.

Abstract: Disclosed herein are modified plant zinc finger proteins; compositions comprising modified plant zinc finger proteins and methods of making and using modified plant zinc finger proteins. The modified plant zinc finger proteins, in contrast to naturally-occurring plant zinc finger proteins, have a binding specificity that is determined by tandem arrays of modular zinc finger binding unit.

Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.