Abstract: Vector constructs for expression of two or more functional proteins or polypeptides under operative control of a single promoter and methods of making and using the same are described. The vectors comprise a self-processing cleavage site between each respective protein or polypeptide coding sequence. The vector constructs include the coding sequence for a self-processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from expressed protein(s) or polypeptide(s). The vector constructs find utility in methods for enhanced production of biologically active proteins and polypeptides in vitro and in vivo.

Abstract: A cooling system for beverage dispense has a circuit 5 in which binary ice is circulated. A modular heat exchange unit 13 for a plurality of beverage lines 25,27,29,31,33 is connected to the circuit 5 for cooling beverage prior to dispense from a dispense tap 35 in a serving area such as a bar. The ice fraction in the binary ice provides a thermal store that can absorb heat without increasing the temperature of the binary ice and enables the system to respond quickly to changes in the cooling requirements in the circuit 5.

Abstract: Lentivector constructs for expression of recombinant proteins, polypeptides or fragments thereof and methods of making the same are described. The lentivectors typically have a self-processing cleavage sequence between a first and second protein or polypeptide coding sequence allowing for expression of a functional protein or polypeptide under operative control of a single promoter and may further include an additional proteolytic cleavage sequence which provides a means to remove the self-processing cleavage sequence from the expressed protein or polypeptide. The vector constructs find utility in methods relating to enhanced production of biologically active proteins, such as immunoglobulins or fragments thereof in vitro and in vivo.

Abstract: A cracking furnace construction includes a firebox defining a chamber having a high emissivity thermal protective coating disposed on at least part of the refractory walls and/or on process tubes disposed within the chamber. The coating contains an inorganic adhesive for metal/alloy tubes or colloidal silica and/or colloidal alumina for refractory walls or ceramic tubes, a filler, and one or more emissivity agents. A method of coating the firebox chamber includes preparing the surface of the tubes/refractory walls, preparing the coating, and applying the coating to the surface.

Abstract: Vector constructs for expression of two or more functional proteins or polypeptides under operative control of a single promoter and methods of making and using the same are described. The vectors comprise a self-processing cleavage site between each respective protein or polypeptide coding sequence. The vector constructs include the coding sequence for a self-processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from expressed protein(s) or polypeptide(s). The vector constructs find utility in methods for enhanced production of biologically active proteins and polypeptides in vitro and in vivo.

Abstract: Cellular compositions and methods for inducing an immune response to tumor cells are described. The cellular compositions include a tumor antigen and cells that have been modified to express a cytokine and one or more of a tumor antigen, anti-CTLA4 antibody and an additional cytokine. The cellular compositions find utility in methods for treating cancer.

Abstract: A post-mix beverage valve provides for automatic, accurate beverage ratioing. A valve body can be assembled, and includes a water flow hard body, syrup body and common nozzle body. The water and syrup flow bodies define flow channels and include one end for connection to water and syrup respectively, and opposite ends for fluid connection to the nozzle body. The water flow channel includes a turbine flow sensor connected to a micro-controller determining the water flow rate. The syrup flow channel includes a flow sensor, two MEMS pressure sensors, monitoring the syrup. The sensors are connected to the micro-controller and positioned about an orifice and senses sense a differential pressure indicative of syrup flow rate solenoid regulates flow of syrup through the syrup body. A stepper motor on the water body controls a rod in the flow channel in conjunction with a V-groove.

Abstract: Recombinant lentiviruses and transfer vectors for transgene delivery as well as methods for gene therapy using such vectors are disclosed. The invention provides a third generation lentiviral packaging system and a set of vectors for producing recombinant lentiviruses, as well as novel tissue specific enhancer and promoter elements useful for optimizing liver specific transgene delivery. The transgene is preferably a blood clotting factor such as human factor IX (hFIX) or human factor VIII (hFVIII) and can be used for treatment of hemophilia.

Abstract: Lentivector constructs for expression of recombinant proteins, polypeptides or fragments thereof and methods of making the same are described. The lentivectors typically have a self-processing cleavage sequence between a first and second protein or polypeptide coding sequence allowing for expression of a functional protein or polypeptide under operative control of a single promoter and may further include an additional proteolytic cleavage sequence which provides a means to remove the self-processing cleavage sequence from the expressed protein or polypeptide. The vector constructs find utility in methods relating to enhanced production of biologically active proteins, such as immunoglobulins or fragments thereof in vitro and in vivo.

Abstract: A coating, method of coating and substrates coated thereby, wherein the coating contains an inorganic adhesive such as an alkali/alkaline earth metal silicate such as sodium silicate, potassium silicate, calcium silicate, and magnesium silicate; a filler such as a metal oxide for example silicon dioxide, aluminum oxide, titanium dioxide, magnesium oxide, calcium oxide and boron oxide; and one or more emissivity agents such as silicon hexaboride, carbon tetraboride, silicon tetraboride, silicon carbide, molybdenum disilicide, tungsten disilicide, zirconium diboride, cupric chromite, or metallic oxides such as iron oxides, magnesium oxides, manganese oxides, chromium oxides and copper chromium oxides, and derivatives thereof. In a coating solution, an admixture of the coating contains water. A stabilizer such as bentonite, kaolin, magnesium alumina silicon clay, tabular alumina and stabilized zirconium oxide may be added.

Abstract: A beverage dispenser to provide a plurality of different flavors with accurate mixing control comprises a housing (40, 42, 44) containing a diluent valve (46A, 46B) and at least two concentrate valves (48A, B, C, D), each valve having its own inlet (12) and outlet (14), characterized in that all the outlets (14) lead to a single dispense nozzle (50A, B, C), a flow rate sensor (16) is provided for each valve, the flow rate sensors (16) being connected to a controller (54), and a setting mechanism (52A, B, C, D, E, F) is provided to open and close each valve, the controller (54) operating the setting mechanisms whereby one concentrate valve and the diluent valve may be opened to dispense a particular beverage and, in response to the sensed flow rates through those opened valves, controlling the degree of opening of those valves to achieve a predetermined diluent to concentrate ratio for the beverage mixture in the dispense nozzle (50A, B, C).

Abstract: A coating admixture, method of coating and substrates coated thereby, wherein the coating contains colloidal silica, colloidal alumina, or combinations thereof; a filler such as silicon dioxide, aluminum oxide, titanium dioxide, magnesium oxide, calcium oxide and boron oxide; and one or more emissivity agents such as silicon hexaboride, carbon tetraboride, silicon tetraboride, silicon carbide, molybdenum disilicide, tungsten disilicide, zirconium diboride, cupric chromite, or metallic oxides such as iron oxides, magnesium oxides, manganese oxides, chromium oxides, copper chromium oxides, cerium oxides, terbium oxides, and derivatives thereof. In a coating solution, an admixture of the coating contains water. A stabilizer such as bentonite, kaolin, magnesium alumina silicon clay, tabular alumina and stabilized zirconium oxide is also added.

Abstract: Vector constructs for expression of two or more functional proteins or polypeptides under operative control of a single promoter and methods of making and using the same are described. The vectors comprise a self-processing cleavage site between each respective protein or polypeptide coding sequence. The vector constructs include the coding sequence for a self-processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from expressed protein(s) or polypeptide(s). The vector constructs find utility in methods for enhanced production of biologically active proteins and polypeptides in vitro and in vivo.

Abstract: A post-mix beverage valve provides for automatic, accurate beverage ratioing. A valve body can be assembled, and includes a water flow hard body, syrup body and common nozzle body. The water and syrup flow bodies define flow channels and include one end for connection to water and syrup respectively, and opposite ends for fluid connection to the nozzle body. The water flow channel includes a turbine flow sensor connected to a micro-controller determining the water flow rate. The syrup flow channel includes a flow sensor, two MEMS pressure sensors, monitoring the syrup. The sensors are connected to the micro-controller and positioned about an orifice and senses sense a differential pressure indicative of syrup flow rate solenoid regulates flow of syrup through the syrup body. A stepper motor on the water body controls a rod in the flow channel in conjunction with a v-groove.

Abstract: A method and apparatus for purifying water by employing steam generated by heating water in a lower section of a container to heat water in an upper section of the container. The lower section is separated from the upper section by a filter. A pressure differential is created across the filter such that a portion of the steam forms a steam pocket below the filter and the remaining portion passes through the filter to heat the water in the upper section. A microprocessor control system is operable to control heating of the water in the lower section and withdrawal of purified water from the upper section to maintain the size of the steam pocket between upper and lower limits.

Abstract: The present invention comprises a valve providing for the dispensing of two liquids at a desired ratio. The valve includes first and second liquid flow body assemblies releasably securable to a nozzle body assembly. Each liquid flow body assembly is securable to a source of liquid and includes flow sensing means and flow regulating means. A control receives inputs from the flow sensing means and regulates the operation of the flow regulating means to provide for the dispensing of the two liquids from the nozzle body assembly at a predetermined ratio.

Abstract: The present invention is a valve that provides for automatic and accurate fluid dispensing of two fluids at a predetermined ratio. The valve is designed to be easily assembled and disassembled by hand without the need for hand tools, and includes a diluent flow body and a concentrate flow body releasably securable to a common nozzle body portion. The diluent and concentrate flow bodies each define a flow channel and each include a first end for connection to sources of diluent and concentrate respectively, and an opposite end for fluid connection with the nozzle body. The diluent body flow channel includes a flow sensor connected to a micro-controller for sensing the flow rate of the diluent. The concentrate body flow channel includes a further flow sensor for sensing the flow rate of the concentrate. Stepper motors are secured to both the diluent and concentrate bodies and each operates a linear rod extending there through and through a flow regulator.

Abstract: Apparatus for mixing two fluids has separate supply lines for delivering the fluids to a mixing head. Flow rate of fluid in one supply line is monitored by arranging a pressure transducer to measure a pressure drop across an orifice in an orifice plate arranged in the supply line and a controller is responsive to the detected flow rate to control flow rate of fluid in the other supply line to achieve a desired flow ratio of both fluids at the mixing head for producing a mixed fluid having the correct proportions of both fluids. The fluids may be a concentrate syrup and a plain or carbonated water to produce a beverage.

Abstract: The present invention is dispensing valve that provides for automatic and accurate fluid ratioing of two fluids. A valve body is designed to be easily assembled and disassembled by hand without the need for hand tools, and includes a first liquid flow body and a second liquid flow body releasably securable to a common nozzle body portion. The first and second liquid flow bodies each include flow sensors and the first liquid flow rate is adjusted to the sensed second liquid flow rate. The flow rate of the first liquid is regulated by a linear actuator operating a shaft through an orifice located in a flow channel of the first liquid flow body. The position of an end of the shaft relative to the orifice regulates the cross-sectional size of the flow channel through the orifice and therefor regulates the flow rate of the first liquid as a function of that cross-sectional size. The flow of the second liquid is regulated by an on/off device.

Abstract: The present invention is a flow rate control valve through which a liquid flows and from which the liquid is dispensed. The flow rate of the liquid is sensed and that information is sent to a microprocessor based control. The control operates a drive of the control valve so as to vary the resultant rate at which the liquid is dispensed therefrom. The drive operates a piston closure member that extends closely within a cylindrical passageway. The passageway has an inlet end and an outlet end and the drive operates the closure member in the passageway to a plurality of positions from a first position at the inlet end and a second position at the outlet end.