Abstract: Systems and methods are disclosed. An example method of determining the concentration of at least one analyte in a plurality of samples by sequentially subjecting each sample to an analysis cycle includes contacting the sample or a sample-derived solution with a sensor surface supporting a species capable of specifically binding the analyte or an analyte-binding species, detecting the amount of binding to the sensor surface, and regenerating the sensor surface to prepare it for the next analytical cycle, and based on the detected binding to the sensor surface determining the concentration of analyte in each sample using virtual calibration data calculated for each analysis cycle from real calibration data obtained by contacting the solid phase with samples containing known concentrations of analyte.

Abstract: A method of determining the concentration of at least one analyte in a plurality of samples by sequentially subjecting each sample to an analysis cycle comprises contacting the sample or a sample-derived solution with a sensor surface supporting a species capable of specifically binding the analyte or an analyte-binding species, detecting the amount of binding to the sensor surface, and regenerating the sensor surface to prepare it for the next analytical cycle, and based on the detected binding to the sensor surface determining the concentration of analyte in each sample using virtual calibration data calculated for each analysis cycle from real calibration data obtained by contacting the solid phase with samples containing known concentrations of analyte.

Abstract: A system and a method for determining a concentration region for a measurement of a response value by means of a calibration curve is disclosed. The calibration curve includes response values as a function of concentrations. The method includes providing a series of measured calibration data; fitting a regression model to the series of measured calibration data; calculating a standard deviation and a standard error for the measured calibration data; calculating a probability using the t-distribution with parameters including degrees of freedom and a requested confidence interval; calculating a response value interval; applying the response value interval to the calibration model; measuring a response value for a sample; and determining the concentration region by means of the response value interval and the calibration model.

Abstract: A method of determining the concentration of at least one analyte in a plurality of samples by sequentially subjecting each sample to an analysis cycle comprises contacting the sample or a sample-derived solution with a sensor surface supporting a species capable of specifically binding the analyte or an analyte-binding species, detecting the amount of binding to the sensor surface, and regenerating the sensor surface to prepare it for the next analytical cycle, and based on the detected binding to the sensor surface determining the concentration of analyte in each sample using virtual calibration data calculated for each analysis cycle from real calibration data obtained by contacting the solid phase with samples containing known concentrations of analyte.

Abstract: A method of determining the concentration of a virus or antigen thereof in a sample comprises the steps of: providing a sensor surface having immobilized thereto a virus antigen or a virus antigen analogue, mixing the sample with a known amount of antibody to the virus antigen to obtain a predetermined concentration of antibody to the antigen in the sample mixture, contacting the sample mixture with the sensor surface to bind free antibody in the mixture to the sensor surface, measuring the response of the sensor surface to the binding of free antibody, and determining the concentration of the virus or antigen in the sample from a calibration curve prepared by measuring the responses obtained for mixtures containing the predetermined concentration of antibody and different concentrations of virus.