Abstract: The invention comprises several embodiments of a multi-step method for processing hard tissues, including bone, teeth, tooth enamel and dentin, mollusk shells, crustacean shells, deep sea sponge, coral, radiolarians, diatoms, antler bone, and other naturally occurring mineralized biocomposites, and soft tissues, including connective tissue, cartilage, tendon, ligament, vertebral discs, pathological mineralized soft tissues and other soft tissues, including at least processing a portion of tissue by one or more of contacting, immersing, adding and mixing a portion of tissue with a first solution, obtaining a first material, derived from processing the portion of tissue with the first solution, processing the first material using one or more alkali metal and ammonium salts of citrate, acetate, phosphate, sulfate, tartarate and chloride, and obtaining a resulting material, derived from processing the first material with the one or more salts.

Abstract: The invention comprises several embodiments of a multi-step method for processing hard tissues, including bone, teeth, tooth enamel and dentin, mollusk shells, crustacean shells, deep sea sponge, coral, radiolarians, diatoms, antler bone, and other naturally occurring mineralized biocomposites, and soft tissues, including connective tissue, cartilage, tendon, ligament, vertebral discs, pathological mineralized soft tissues and other soft tissues, including at least processing a portion of tissue by one or more of contacting, immersing, adding and mixing a portion of tissue with a first solution, obtaining a first material, derived from processing the portion of tissue with the first solution, processing the first material using one or more alkali metal and ammonium salts of citrate, acetate, phosphate, sulfate, tartarate and chloride, and obtaining a resulting material, derived from processing the first material with the one or more salts.

Abstract: The invention comprises several embodiments of a multi-step method for extracting mineral and protein components from a mineralized biocomposite material, including at least the steps of: extracting proteins and minerals from a material into a first solution by contacting or immersing it with the first solution, comprising one or more of a demineralization solution and a salt solution; obtaining a first solid portion from the first solution, by separating the solid and liquid portions; extracting proteins and minerals from the first solid portion into a second solution by contacting or immersing it with the second solution, comprising one or more of a demineralization solution and a salt solution; and obtaining a second solid portion from the second solution by separating the liquid and solid portions. Each of these embodiments may be operated in succession to any of the other embodiments to create custom optimized extraction processes.