Abstract: Methods for additive manufacturing of an object are provided which comprise providing a build solution comprising proteins and a solvent in an additive manufacturing system; generating heat in the build solution to denature proteins; and aggregating denatured proteins to form a solidified region of an object, the solidified region comprising denatured, aggregated proteins. The heat may be generated in a localized area of the build solution to denature proteins in the localized area, wherein the localized area is in contact with unheated build solution and the solidified region may be formed in the localized area of the build solution.