Patents by Inventor Ayako YOKOI

Ayako YOKOI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11155826
    Abstract: The present inventors found that the efficiency of introducing a protein into a plant dramatically increases as compared with conventional methods for cultivating a bacteria-infected plant as it is when the protein of interest is expressed with bacteria having the type III secretion system, the bacteria are brought into contact with the plant, and then the infected tissues are cultured under bacteriostatic conditions for a certain period of time.
    Type: Grant
    Filed: December 25, 2017
    Date of Patent: October 26, 2021
    Assignee: National Agriculture and Food Research Organization
    Inventors: Ichiro Mitsuhara, Yuki Yanagawa, Kasumi Yamada, Seiichi Toki, Ayako Yokoi
  • Publication number: 20200283781
    Abstract: The present inventors found that the efficiency of introducing a protein into a plant dramatically increases as compared with conventional methods for cultivating a bacteria-infected plant as it is when the protein of interest is expressed with bacteria having the type III secretion system, the bacteria are brought into contact with the plant, and then the infected tissues are cultured under bacteriostatic conditions for a certain period of time.
    Type: Application
    Filed: December 25, 2017
    Publication date: September 10, 2020
    Applicant: National Agriculture and Food Research Organization
    Inventors: Ichiro MITSUHARA, Yuki YANAGAWA, Kasumi YAMADA, Seiichi TOKI, Ayako YOKOI
  • Publication number: 20200170920
    Abstract: A cosmetic is provided, including a component (R): synthetic rubber particles, and a component (P): a polymer having a repeating unit (u1) represented by General Formula (p1). In General Formula (p1), R01, R02 and R03 each independently represents an alkyl group or a hydrogen atom; np represents an integer of 1 to 4; R10 represents a substituent; and mp represents an integer of 0 to (np+1).
    Type: Application
    Filed: July 12, 2018
    Publication date: June 4, 2020
    Inventors: Keiichi NAGAI, Ayako YOKOI
  • Patent number: 10323251
    Abstract: A method for producing a plant cell comprising a mutation introduced in a target DNA comprises: a step of introducing into plant cells a DNA construct comprising a DNA homologous to a target DNA, wherein a desired mutation is introduced and a piggyBac transposon containing a marker gene is inserted in the homologous DNA; a step of selecting a plant cell, in which the mutation and the piggyBac transposon are introduced in the target DNA via homologous recombination, based on an expression of the marker gene; and a step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.
    Type: Grant
    Filed: February 19, 2015
    Date of Patent: June 18, 2019
    Assignee: NATIONAL RESEARCH AND DEVELOPMENT AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
    Inventors: Ayako Yokoi, Seiichi Toki
  • Publication number: 20170058285
    Abstract: A method for producing a plant cell comprising a mutation introduced in a target DNA comprises: a step of introducing into plant cells a DNA construct comprising a DNA homologous to a target DNA, wherein a desired mutation is introduced and a piggyBac transposon containing a marker gene is inserted in the homologous DNA; a step of selecting a plant cell, in which the mutation and the piggyBac transposon are introduced in the target DNA via homologous recombination, based on an expression of the marker gene; and a step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.
    Type: Application
    Filed: February 19, 2015
    Publication date: March 2, 2017
    Applicant: NATIONAL RESEARCH AND DEVELOPMENT AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
    Inventors: Ayako YOKOI, Seiichi TOKI