Abstract: Disclosed is a method of producing a target protein having improved solubility and folding efficiency, characterized by expressing a target protein as a fusion protein using an RNA-binding protein as a fusion partner, and binding the fusion partner fused to the target protein to an RNA molecule. More particularly, the present invention discloses a method of producing a target protein having improved solubility and folding efficiency, comprising constructing an expression vector encoding a fusion protein using a target protein and an RNA-binding protein as a fusion partner of the target protein; constructing an expression vector expressing an RNA molecule capable of binding to the RNA-binding protein; and cotransforming a host cell with the expression vectors and then culturing the resulting transformant, wherein the expressed fusion protein binds to the overexpressed RNA molecule. The method of the present invention is very useful for production of proteins for medical and industrial applications.