Patents by Inventor Bart W. Nieuwenhuijsen

Bart W. Nieuwenhuijsen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • PCR-DIRECTED GENE SYNTHESIS FROM LARGE NUMBER OF OVERLAPPING OLIGODEOXYRIBONUCLEOTIDES
    Publication number: 20080182296
    Abstract: The present invention provides methods of PCR-directed gene synthesis that may be used for all genes, including those with a high G+C content and/or a long sequence. The invention relates to methods of gene synthesis using overlapping oligonucleotides and polymerase chain reaction (PCR), wherein several PCR parameters, e.g., the concentration of overlapping oligonucleotides, the type of DNA polymerase used, and the number of PCR amplification cycles, are optimized. Additionally, the invention relates to oligonucleotide design that allows for increased protein expression of synthesized genes.
    Type: Application
    Filed: January 31, 2008
    Publication date: July 31, 2008
    Inventors: Pranab K. Chanda, Bart W. Nieuwenhuijsen