Patents by Inventor Bhanu Chandra MULUKUTLA
Bhanu Chandra MULUKUTLA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240093248Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.Type: ApplicationFiled: July 12, 2023Publication date: March 21, 2024Applicant: Pfizer Inc.Inventors: Gregory Walter HILLER, Jeffrey Joseph MITCHELL, Bhanu Chandra MULUKUTLA, Pamela Mary PEGMAN
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Publication number: 20240016915Abstract: The invention relates to methods for producing an RSV F protein trimer in a fed batch cell culture.Type: ApplicationFiled: September 30, 2021Publication date: January 18, 2024Applicant: Pfizer Inc.Inventors: Shelby Hutchins BREEN, Cameron Albert HARRINGTON, Michaela Evelina JACOBS, Jason Arnold LOTVIN, Bhanu Chandra MULUKUTLA, David STEAD, Madhuresh SUMIT
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Patent number: 11718864Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.Type: GrantFiled: June 30, 2020Date of Patent: August 8, 2023Assignee: Pfizer Inc.Inventors: Gregory Walter Hiller, Jeffrey Joseph Mitchell, Bhanu Chandra Mulukutla, Pamela Mary Pegman
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Publication number: 20220073942Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.Type: ApplicationFiled: December 3, 2019Publication date: March 10, 2022Applicant: PFIZER INC.Inventors: Emily Ann DEUTSCHMAN, Jeffrey Joseph MITCHELL, Bhanu Chandra MULUKUTLA, John Joseph SCARCELLI
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Publication number: 20210017553Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.Type: ApplicationFiled: June 30, 2020Publication date: January 21, 2021Applicant: PFIZER INC.Inventors: Gregory Walter HILLER, Jeffrey Joseph MITCHELL, Bhanu Chandra MULUKUTLA, Pamela Mary PEGMAN
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Patent number: 10738334Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.Type: GrantFiled: September 22, 2016Date of Patent: August 11, 2020Assignee: Pfizer Inc.Inventors: Gregory Walter Hiller, Jeffrey Joseph Mitchell, Bhanu Chandra Mulukutla, Pamela Mary Pegman
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Publication number: 20200239852Abstract: A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3 mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2 mM.Type: ApplicationFiled: December 20, 2019Publication date: July 30, 2020Applicant: Pfizer Inc.Inventors: Gregory Walter Hiller, Bhanu Chandra Mulukutla
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Publication number: 20200056190Abstract: Provided herein is a tyrosine selection marker system, and uses thereof. In some embodiments, nucleic acid constructs, vectors, host cells and related compositions and methods for generating and selecting tyrosine prototroph cells are provided.Type: ApplicationFiled: March 9, 2018Publication date: February 20, 2020Applicant: PFIZER INC.Inventors: Gregory Walter HILLER, Jeffrey Joseph MITCHELL, Bhanu Chandra MULUKUTLA, Pamela Mary PEGMAN
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Patent number: 10544395Abstract: A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3 mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2 mM.Type: GrantFiled: March 17, 2015Date of Patent: January 28, 2020Assignee: Pfizer Inc.Inventors: Gregory Walter Hiller, Bhanu Chandra Mulukutla
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Publication number: 20180265904Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.Type: ApplicationFiled: September 22, 2016Publication date: September 20, 2018Applicant: PFIZER INC.Inventors: GREGORY WALTER HILLER, JEFFREY JOSEPH MITCHELL, BHANU CHANDRA MULUKUTLA, PAMELA MARY PEGMAN
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Publication number: 20170107552Abstract: A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3 mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2 mM.Type: ApplicationFiled: March 17, 2015Publication date: April 20, 2017Inventors: Gregory Walter HILLER, Bhanu Chandra MULUKUTLA