Patents by Inventor Bianca Elisabeth Maria Gielesen

Bianca Elisabeth Maria Gielesen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11149268
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, René Verwaal, Bianca Elisabeth Maria Gielesen, Brenda Vonk
  • Patent number: 11149288
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Publication number: 20210198701
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Application
    Filed: March 12, 2021
    Publication date: July 1, 2021
    Inventors: Paul KLAASSEN, Gijsberdina Pieternella VAN SUYLEKOM, Bianca Elisabeth Maria GIELESEN, Nicolette Jasmijn BROERS, Beate WIEDEMANN, Wilhelmus Theodorus Antonius Maria DE LAAT
  • Patent number: 10982235
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: April 20, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
  • Publication number: 20200199599
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a guide-RNA expression system for a eukaryotic host cell.
    Type: Application
    Filed: July 17, 2017
    Publication date: June 25, 2020
    Inventors: Eric Mosher YOUNG, Amar GHODASARA, René VERWAAL, Johannes Andries ROUBOS, Bianca Elisabeth Maria GIELESEN, Brenda VONK, Alec A.K. NIELSEN, Christopher Ashby VOIGT
  • Publication number: 20200165637
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: May 28, 2020
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS
  • Patent number: 10590436
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: March 17, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Publication number: 20200032252
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a self-guiding integration construct for a genome editing system.
    Type: Application
    Filed: April 4, 2018
    Publication date: January 30, 2020
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Brenda VONK
  • Publication number: 20190194692
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: June 27, 2019
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS, Carsten POHL, Arnold Jacob Mattieu DRIESSEN, Roelof Ary Lans BOVENBERG, Yvonne Irene NYGÅRD
  • Publication number: 20190177748
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, arae and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Application
    Filed: February 22, 2019
    Publication date: June 13, 2019
    Inventors: Paul KLAASSEN, Gijsberdina Pieternella VAN SUYLEKOM, Bianca Elisabeth Maria GIELESEN, Nicolette Jasmijn BROERS, Beate WIEDEMANN, Wilhelmus Theodorus Antonius Maria DE LAAT
  • Patent number: 10260075
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Grant
    Filed: January 17, 2017
    Date of Patent: April 16, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
  • Publication number: 20180023096
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 25, 2018
    Applicant: DSM IP ASSETS B.V.
    Inventors: BERNARD MEIJRINK, RENÉ VERWAAL, BIANCA ELISABETH MARIA GIELESEN, JOHANNES ANDRIES ROUBOS
  • Publication number: 20170121738
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Application
    Filed: January 17, 2017
    Publication date: May 4, 2017
    Inventors: Paul KLAASSEN, Gijsberdina Pieternella VAN SUYLEKOM, Bianca Elisabeth Maria GIELESEN, Nicolette Jasmijn BROERS, Beate WIEDEMANN, Wilhelmus Theodorus Antonius Maria DE LAAT
  • Patent number: 9551015
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia,: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Grant
    Filed: July 6, 2010
    Date of Patent: January 24, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
  • Patent number: 9499841
    Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: November 22, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Wilbert Herman Marie Heijne
  • Patent number: 9334504
    Abstract: The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.
    Type: Grant
    Filed: February 27, 2014
    Date of Patent: May 10, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Marco Alexander Van Den Berg, Bianca Elisabeth Maria Gielesen, Adriana Marina Riemens
  • Publication number: 20150291983
    Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.
    Type: Application
    Filed: June 25, 2015
    Publication date: October 15, 2015
    Inventors: Paul KLAASSEN, Bianca Elisabeth Maria GIELESEN, Gijsberdina Pieternella VAN SUYLEKOM, Wilbert Herman Marie HEIJNE
  • Patent number: 9096675
    Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.
    Type: Grant
    Filed: April 19, 2011
    Date of Patent: August 4, 2015
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Wilbert Herman Marie Heijne
  • Publication number: 20140178932
    Abstract: The present invention provides a method for the transfection of filamentous fungal cells, comprising providing a multitude of containers, filling into each container an amount of polymer needed for the transfection, filling the cells to be transfected as well as an aqueous solution of transfection reagent into each of the containers, incubating the resulting mixture, removing the transfection reagent from the incubated mixture; and selecting the cells which have been transformed, characterized in that the total volume of the incubating mixture is less than 1 ml per container. Furthermore, the present invention provides the use of transformed filamentous fungal cells for the production of proteins or metabolites.
    Type: Application
    Filed: February 27, 2014
    Publication date: June 26, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Marco Alexander Van Den Berg, Bianca Elisabeth Maria Gielesen, Adriana Marina Riemens
  • Publication number: 20140141473
    Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.
    Type: Application
    Filed: April 20, 2012
    Publication date: May 22, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve