Abstract: This patent application discloses siRNA sequences against the constant region of the ,8 influenza virus nucleoprotein gene comprising: (SEQ ID NO: 1) Sense strand: 5??UGAAGGAUCUUAUUUCUUCdTdT 3? (SEQ ID NO: 2) Anti sense strand: 3??dTdTACUUCCUAGAAUAAAGAAG 5? or (SEQ ID NO: 3) Sense strand: 5??UGAAGGAUCUUAUUUCUUCGGdTdT 3? (SEQ ID NO: 4) Anti sense strand: 3??dTdTACUUCCUAGAAUAAAGAAGCC 5? or (SEQ ID NO: 5) Sense strand: 5??GGAUCUUAUUUCUUCGGAGACdTdT 3? (SEQ ID NO: 6) Anti sense strand: 3??dTdTCCUAGAAUAAAGAAGCCUCUG 5? said sequences being inhibitory against influenza virus in animals including humans. The invention further includes one or more of said siRNA sequences in the form of an aqueous suspension suitable for nasal inhalation. Still further, the invention includes one or more of said siRNA sequences in the form of a plasmid expressing intracellularly in animals including humans.

Abstract: This patent application discloses siRNA sequences against the constant region of the influenza virus nucleoprotein gene comprising: Sense strand: 5??UGAAGGAUCUUAUUUCUUCdTdT 3? (SEQ ID NO: 1) Anti sense 3??dTdTACUUCCUAGAAUAAAGAAG 5? strand: (SEQ ID NO: 2) or Sense strand: 5??UGAAGGAUCUUAUUUCUUCGGdTdT 3? (SEQ ID NO: 3) Anti sense 3??dTdTACUUCCUAGAAUAAAGAAGCC 5? strand: (SEQ ID NO: 4) or Sense strand: 5??GGAUCUUAUUUCUUCGGAGACdTdT 3? (SEQ ID NO: 5) Anti sense 3??dTdTCCUAGAAUAAAGAAGCCUCUG 5? strand: (SEQ ID NO: 6) said sequences being inhibitory against influenza virus in animals including humans. The invention further includes one or more of said siRNA sequences in the form of an aqueous suspension suitable for nasal inhalation. Still further, the invention includes one or more of said siRNA sequences in the form of a plasmid expressing intracellularly in animals including humans.

Abstract: This patent application discloses siRNA sequences against the constant region of the influenza virus nucleoprotein gene comprising: Sense strand: 5? UGAAGGAUCUUAUUUCUUCdTdT 3? (SEQ ID NO: 1) Anti sense strand: 3? dTdTACUUCCUAGAAUAAAGAAG 5? (SEQ ID NO: 2) Sense strand: 5? UGAAGGAUCUUAUUUCUUCGGdTdT 3? (SEQ ID NO: 3) Anti sense strand: 3? dTdTACUUCCUAGAAUAAAGAAGCC 5? (SEQ ID NO: 4) Sense strand: 5? GGAUCUUAUUUCUUCGGAGACdTdT 3? (SEQ ID NO: 5) Anti sense strand: 3? dTdTCCUAGAAUAAAGAAGCCUCUG 5? (SEQ ID NO: 6) said sequences being inhibitory against influenza virus in animals including humans. The invention further includes one or more of said siRNA sequences in the form of an aqueous suspension suitable for nasal inhalation. Still further, the invention includes one or more of said siRNA sequences in the form of a plasmid expressing intracellularly in animals including humans.

Abstract: A rapid, non-radioactive approach to the diagnosis of sickle cell anemia is described based on an allele specific polymerase chain reaction (ASPCR) in which the 3'-terminal nucleotide of one of the primers of the primer set forms a match with one allele and a mismatch with the other allele. This method allows the direct detection of the normal or the sickle cell .beta.-globin allele in genomic DNA without the additional steps of probe hybridization, ligation or restriction enzyme cleavage.