Patents by Inventor Blake A. Simmons

Blake A. Simmons has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11414653
    Abstract: The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: August 16, 2022
    Assignees: National Technology & Engineering Solutions of Sandia, LLC, Battelle Memorial Institute, The Regents of the University of California
    Inventors: John M. Gladden, Saori Amaike Campen, Jinxiang Zhang, Jon K. Magnuson, Scott E. Baker, Blake A. Simmons
  • Publication number: 20220194877
    Abstract: The present invention provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent comprising a polyamine, or a mixture of polyamines, to a biomass to dissolve at least part of solid biomass in the solvent, wherein the polyamine is a Bronsted or Lewis base, and/or the polyamine is a hydrogen bond donor and/or acceptor; (b) optionally introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; (c) optionally separating the sugar from the solubilized biomass mixture; and (d) optionally separating the lignan from the solubilized biomass mixture.
    Type: Application
    Filed: December 9, 2021
    Publication date: June 23, 2022
    Inventors: Ezinne Achinivu, Mood Mohan, Hemant Choudhary, Lalitendu Das, Venkataramana R. Pidatala, Harsha D. Magurudeniya, Kaixuan Huang, John M. Gladden, Blake A. Simmons
  • Publication number: 20220195666
    Abstract: A method to deconstruct a biomass: the method comprising: introducing a solvent comprising a plurality of salt ionic liquid (PSIL) (such as a double salt ionic liquid (DSIL)) to a biomass to dissolve at least part of solid biomass in the solvent; wherein the PSIL (or DSIL) is an organic salt comprising three or more ions, and the PSIL comprises: (i) a hard anion ionic liquid (IL) and a soft anion IL, (ii) at least one IL having a pKa value of equal to or higher than 10, or (iii) at least one IL has a low hydrogen bond donor ability.
    Type: Application
    Filed: December 22, 2021
    Publication date: June 23, 2022
    Inventors: Hemant Choudhary, Harsha D. Magurudeniya, John M. Gladden, Blake A. Simmons
  • Publication number: 20220170053
    Abstract: In one aspect, the present invention provides methods for preparing a fermentation product. The methods include pre-treating a mixture of biomass and ionic liquid, wherein the ionic liquid comprises a choline cation and the biomass comprises polysaccharide and lignin. The methods further include forming hydrolysates from the introduction of glycoside hydrolase to the pre-treated mixture at conditions sufficient to produce a sugar composition mixture for fermentation steps. The present invention provides methods for loading biomass mixtures in a batch-fed process, wherein the biomass slurries can be loaded into water or a concentrated sugar composition for hydrolysate production. The methods can be performed in a one-pot process, wherein the ionic liquids are present in the mixtures throughout each step. Aspects of the invention provide compositions of sugar composition mixtures and fermentation product mixtures.
    Type: Application
    Filed: September 13, 2021
    Publication date: June 2, 2022
    Inventors: Feng Xu, Blake Simmons, Seema Singh
  • Patent number: 11312978
    Abstract: The present invention relates to methods of upgrading biomass to provide useful chemical intermediates, fuels, amino acids, nutrients, etc. In particular examples, the biomass is a by-product of ethanol production and is mainly used as high-protein feed. Described herein are methods for upgrading such biomass, such as by implementing pre-treatment conditions and by employing fermentation conditions including modified organisms.
    Type: Grant
    Filed: May 7, 2020
    Date of Patent: April 26, 2022
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Ryan Wesley Davis, Blake Simmons, Mary Bao Tran-Gyamfi, Benjamin Chiau-Pin Wu
  • Patent number: 11254637
    Abstract: Disclosed herein are lignin-derived ionic liquids and methods for preparing them. The methods include forming a reaction mixture comprising a lignin-derived starting material, a carbonyl compound, and an amine; maintaining the reaction mixture under conditions sufficient to form a lignin-derived aminophenol; and converting the lignin derived aminophenol to the lignin-derived ionic liquid. Monomeric phenols, oligomeric phenols, and polymeric phenols can be used as lignin-derived starting materials.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: February 22, 2022
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Tanmoy Dutta, Jian Sun, Blake A. Simmons, Seema Singh
  • Publication number: 20210363696
    Abstract: The present invention provides for a method to deconstruct a biomass comprising: (a) introducing one or more individual components of an ionic liquid (IL) or deep eutectic solvent (DES) to a biomass, wherein the one or more individual components, and optionally any components already present in the biomass, form an IL or DES, or mixture thereof, which solubilizes the biomass to form a solubilized biomass mixture, wherein at least one individual component is introduced to the biomass separately from any other individual component; (b) optionally introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; and, (c) optionally separating the sugar from the solubilized biomass mixture.
    Type: Application
    Filed: June 4, 2021
    Publication date: November 25, 2021
    Inventors: Harsha D. Magurudeniya, Ezinne Achinivu, Blake A. Simmons, John M. Gladden
  • Publication number: 20210332530
    Abstract: The present invention provides for a method to deconstruct a biomass: the method comprising: (a) ensiling a biomass to produce comprising one or more organic acids, and (b) introducing a solvent to the ensiled biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent is an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, to form a solubilized biomass mixture.
    Type: Application
    Filed: April 27, 2021
    Publication date: October 28, 2021
    Inventors: Harsha D. Magurudeniya, Alberto Rodriguez, Nawa Raj Baral, Blake A. Simmons, John M. Gladden
  • Publication number: 20210317481
    Abstract: The present invention also provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent to a biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent comprises (i) a metal salt, and (ii) an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, to form a solubilized biomass mixture; (b) optionally introducing an enzyme and/or a microbe to the composition such that the enzyme and/or microbe produce a biofuel and/or chemical compound from the solubilized biomass; and, (c) optionally the biofuel and/or chemical compound is separated from the composition.
    Type: Application
    Filed: April 1, 2021
    Publication date: October 14, 2021
    Inventors: Lalitendu Das, Hemant Choudhary, John M. Gladden, Blake A. Simmons
  • Publication number: 20210284676
    Abstract: The present invention provides novel and improved processes for treating a lignocellulosic biomass or technical lignin using ionic liquids to obtain lignin breakdown products and polysaccharide biomass components. Recycling of ionic liquids can be included in the methods of the invention.
    Type: Application
    Filed: March 2, 2021
    Publication date: September 16, 2021
    Inventors: Noppadon Sathitsuksanoh, Ning Sun, Jian Shi, Anthe George, Manali Sawant, Seema Singh, Blake Simmons
  • Patent number: 11118197
    Abstract: In one aspect, the present invention provides methods for preparing a fermentation product. The methods include pre-treating a mixture of biomass and ionic liquid, wherein the ionic liquid comprises a choline cation and the biomass comprises polysaccharide and lignin. The methods further include forming hydrolysates from the introduction of glycoside hydrolase to the pre-treated mixture at conditions sufficient to produce a sugar composition mixture for fermentation steps. The present invention provides methods for loading biomass mixtures in a batch-fed process, wherein the biomass slurries can be loaded into water or a concentrated sugar composition for hydrolysate production. The methods can be performed in a one-pot process, wherein the ionic liquids are present in the mixtures throughout each step. Aspects of the invention provide compositions of sugar composition mixtures and fermentation product mixtures.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: September 14, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Feng Xu, Blake Simmons, Seema Singh
  • Patent number: 11118204
    Abstract: In one aspect, the present invention provides a method for preparing a sugar composition. The method includes: forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and wherein the ionic liquid contains a dicarboxylic acid anion and a cation. The pH of the mixture is greater than or equal to about 10, and the molar ratio of the dicarboxylic acid anion to the cation is at least about 1:2. The method further includes: maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; reducing the pH of the mixture containing the dissolved polysaccharide to at least about 7; adding at least one glycoside hydrolase to the mixture having the reduced pH.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: September 14, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Michael Liszka, Kenneth Sale, Blake Simmons
  • Publication number: 20210269810
    Abstract: The present invention provides for a genetically modified fungal host cell capable of producing prespatane and/or epi-isozizaene comprising prespatane synthase (PPS) and/or epi-isozizaene synthase (EIZS).
    Type: Application
    Filed: February 26, 2021
    Publication date: September 2, 2021
    Inventors: Gina Marie Geiselman, James Kirby, Taek Soon Lee, John M. Gladden, Blake A. Simmons
  • Publication number: 20210214757
    Abstract: The present invention provides for a method of fermenting or saccharifying a biomass comprising: (a) (i) contacting a biomass comprising a polysaccharide, and an ionic liquid (IL) to form a first solution, or (ii) providing the first solution comprising the biomass and the IL, (b) contacting the first solution and carbon dioxide such that the first solution results in a lower pH, (c) introducing (i) an enzyme capable of enzymatically to breakdown at least one bond in the polysaccharide or a breakdown product of the polysaccharide, and/or (ii) a microorganism that capable of producing the enzyme and/or fermenting the polysaccharide or a breakdown product of the polysaccharide, such that the polysaccharide is at least partially broken down and the first solution is transformed into a second solution.
    Type: Application
    Filed: November 16, 2020
    Publication date: July 15, 2021
    Inventors: Jian Sun, Jian Shi, Blake A. Simmons, Seema Singh
  • Publication number: 20210198708
    Abstract: Disclosed herein are methods for preparing sugar compositions. The methods include: i) forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and the ionic liquid contains a) a cation and b) a sugar acid anion or a ketoacid anion; ii) maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; iii) adding at least one glycoside hydrolase to the mixture; and iv) maintaining the mixture containing the glycoside hydrolase under conditions sufficient to hydrolyze at least a portion of the dissolved polysaccharide, thereby forming the sugar compositions. The sugar compositions contain at least one monosaccharide or oligosaccharide. New sugar-based ionic liquids are also described.
    Type: Application
    Filed: March 2, 2021
    Publication date: July 1, 2021
    Inventors: John M. Gladden, Tanmoy Dutta, Jian Sun, Seema Singh, Blake A. Simmons
  • Patent number: 11028249
    Abstract: Methods and compositions are provided for synthesizing ionic liquids from lignin. Methods and compositions are also provided for treating lignin with ionic liquids.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: June 8, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Tanmoy Dutta, Blake Simmons, Seema Singh
  • Publication number: 20210155964
    Abstract: Methods and compositions are provided for ionic liquid processing of biomass.
    Type: Application
    Filed: February 1, 2021
    Publication date: May 27, 2021
    Inventors: Parthasarathi Ramakrishnan, Tanmoy Dutta, Blake Simmons, Seema Singh
  • Patent number: 10934568
    Abstract: Disclosed herein are methods for preparing sugar compositions. The methods include: i) forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and the ionic liquid contains a) a cation and b) a sugar acid anion or a ketoacid anion; ii) maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; iii) adding at least one glycoside hydrolase to the mixture; and iv) maintaining the mixture containing the glycoside hydrolase under conditions sufficient to hydrolyze at least a portion of the dissolved polysaccharide, thereby forming the sugar compositions. The sugar compositions contain at least one monosaccharide or oligosaccharide. New sugar-based ionic liquids are also described.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: March 2, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: John M. Gladden, Tanmoy Dutta, Jian Sun, Seema Singh, Blake A. Simmons
  • Patent number: 10934321
    Abstract: The present invention provides novel and improved processes for treating a lignocellulosic biomass or technical lignin using ionic liquids to obtain lignin breakdown products and polysaccharide biomass components. Recycling of ionic liquids can be included in the methods of the invention.
    Type: Grant
    Filed: January 22, 2019
    Date of Patent: March 2, 2021
    Assignees: The Regents of the University of California, National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Noppadon Sathitsuksanoh, Ning Sun, Jian Shi, Anthe George, Manali Sawant, Seema Singh, Blake Simmons
  • Patent number: 10907184
    Abstract: Methods and compositions are provided for ionic liquid processing of biomass.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: February 2, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Parthasarathi Ramakrishnan, Tanmoy Dutta, Blake Simmons, Seema Singh