Abstract: The present disclosure relates to manganese dioxide nanoparticles and their use in treatment of oxidative stress and conditions related to or characterized by oxidative stress, including osteoarthritis.

Abstract: Compositions and methods are disclosed herein to deliver and anchor nanoscale drug carriers into the extracellular matrix (ECM) of tissues, such as degenerating cartilage or tumor margins, and to provide local and sustained release of drugs.

Abstract: The present invention provides manganese dioxide nanoparticles complexed with one or more nucleic acid sequences for introduction into NK cells for an immunotherapy treatment. The nanoparticles discussed herein target and silence TGF-? in order to reactivate NK cells that have been inactivated or suppressed. Provided is a method of inducing an anti-cancer immune response in a subject, comprising administering to immune cells of the subject a pharmaceutically effective amount of a nanoparticle composition comprising a manganese dioxide nanoparticle complexed with a nucleic acid sequence in an amount sufficient to induce, enhance, or promote an immune response against the cancer in the subject. The invention also comprises a manganese dioxide nanoparticle formulation, comprising the manganese dioxide nanoparticle(s) as described and a pharmaceutically acceptable carrier.

Abstract: The present disclosure relates to manganese dioxide nanoparticles and their use in treatment of oxidative stress and conditions related to or characterized by oxidative stress, including osteoarthritis.

Abstract: Compositions and methods are disclosed herein to deliver and anchor nanoscale drug carriers into the extracellular matrix (ECM) of tissues, such as degenerating cartilage or tumor margins, and to provide local and sustained release of drugs.

Abstract: A multi-layered tissue construct includes: a first layer comprising a first hydrogel; and a second layer comprising a second hydrogel, wherein the first layer is connected to the second layer at a first transition zone and wherein at least one of the first layer and the second layer further comprises a component selected from the group consisting of cells and a bioactive substance. Another multi-layered tissue construct includes: a first layer comprising a first hydrogel; a second layer comprising cells of a first type, wherein the second layer is disposed on the first layer; and a third layer comprising a second hydrogel and optionally cells of the first type encapsulated in the second hydrogel, wherein the third layer is disposed on the second layer. Methods for producing these multi-layered tissue constructs are also disclosed.