Patents by Inventor Brittany A. Rohrman
Brittany A. Rohrman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230357844Abstract: A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.Type: ApplicationFiled: March 16, 2023Publication date: November 9, 2023Inventors: Kurt Patterson, Hari K.K. Subramanian, Brittany A. Rohrman, Fabian Block
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Patent number: 11608528Abstract: A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.Type: GrantFiled: July 21, 2022Date of Patent: March 21, 2023Assignee: Pacific Biosciences of California, Inc.Inventors: Kurt Patterson, Hari K. K. Subramanian, Brittany A. Rohrman, Fabian Block
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Publication number: 20220372552Abstract: A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.Type: ApplicationFiled: June 21, 2022Publication date: November 24, 2022Applicant: Pacific Biosciences of California, Inc.Inventors: Brittany A. ROHRMAN, Denis MALYSHEV, Morassa Mohseni MIDDLETON, Arnold OLIPHANT
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Publication number: 20220356515Abstract: Provided include methods, compositions, kits, and systems for replenishing a rolling circle amplification (RCA) reaction in a vessel. The RCA reaction can be initiated by contacting a nucleic acid template and a primer with a loading buffer comprising a DNA polymerase and polymerase extension agents including a divalent metal cation and a polyelectrolyte, followed by replenishing with an amplification buffer to continue the nucleic acid amplification through primer extension. The amplification buffer is different in composition from the loading buffer and does not comprise any DNA polymerase.Type: ApplicationFiled: May 9, 2022Publication date: November 10, 2022Inventors: Nathan Jameson, Kurt Patterson, Fabian Block, Brittany Rohrman
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Publication number: 20220349002Abstract: A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.Type: ApplicationFiled: July 21, 2022Publication date: November 3, 2022Inventors: Kurt Patterson, Hari K.K. Subramanian, Brittany A. Rohrman, Fabian Block
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Publication number: 20220290216Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.Type: ApplicationFiled: January 4, 2022Publication date: September 15, 2022Applicant: Omniome, Inc.Inventors: Morassa Mohseni MIDDLETON, Mark C. WALLEN, Pinar IYIDOGAN, Michael James SCHMIDT, Brittany A. ROHRMAN, Ying Lin LIU, Fabian BLOCK, Arnold OLIPHANT
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Patent number: 11421262Abstract: A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.Type: GrantFiled: July 22, 2019Date of Patent: August 23, 2022Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.Inventors: Brittany A. Rohrman, Denis Malyshev, Morassa Mohseni Middleton, Arnold Oliphant
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Patent number: 11242557Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.Type: GrantFiled: July 19, 2019Date of Patent: February 8, 2022Assignee: OMNIOME, INC.Inventors: Morassa Mohseni Middleton, Mark C. Wallen, Pinar Iyidogan, Michael James Schmidt, Brittany A. Rohrman, Ying Lin Liu, Fabian Block, Arnold Oliphant
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Publication number: 20200032317Abstract: A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.Type: ApplicationFiled: July 22, 2019Publication date: January 30, 2020Applicant: Omniome, Inc.Inventors: Brittany A. ROHRMAN, Denis MALYSHEV, Morassa Mohseni MIDDLETON, Arnold OLIPHANT
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Publication number: 20190345544Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.Type: ApplicationFiled: July 19, 2019Publication date: November 14, 2019Applicant: Omniome, Inc.Inventors: Morassa Mohseni MIDDLETON, Mark C. WALLEN, Pinar IYIDOGAN, Michael James SCHMIDT, Brittany A. ROHRMAN, Ying Lin LIU, Fabian BLOCK, Arnold OLIPHANT
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Patent number: 10400272Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.Type: GrantFiled: March 15, 2019Date of Patent: September 3, 2019Assignee: OMNIOME, INC.Inventors: Morassa Mohseni Middleton, Mark C. Wallen, Pinar Iyidogan, Michael James Schmidt, Brittany A. Rohrman, Ying Lin Liu, Fabian Block, Arnold Oliphant