Patents by Inventor Bruce P. Neri
Bruce P. Neri has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6692917Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on dendrimers and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: August 27, 2001Date of Patent: February 17, 2004Assignee: Third Wave Technologies, INCInventors: Bruce P. Neri, Jeff G. Hall, Victor Lyamichev, Lloyd M. Smith
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Patent number: 6635463Abstract: The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.Type: GrantFiled: January 11, 2001Date of Patent: October 21, 2003Assignee: Third Wave Technologies, Inc.Inventors: Wu-Po Ma, Victor I. Lyamichev, Michael W. Kaiser, Natalie E. Lyamicheva, Hatim Taysir Allawi, James J. Schaefer, Bruce P. Neri
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Publication number: 20030186238Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: February 26, 2002Publication date: October 2, 2003Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lymaicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
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Publication number: 20030152971Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: November 7, 2002Publication date: August 14, 2003Inventors: Victor Lyamichev, Bruce P. Neri, Jeff Hall, Andrew A. Lukowiak
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Publication number: 20030152942Abstract: The present invention relates to detecting target nucleic acid sequences in pooled samples. In particular, the present invention relates to compositions and methods for detecting the presence or absence of target nucleic acid sequences (e.g. RNA virus sequences) in a pooled sample employing an INVADER detection assay. In certain embodiments, the present invention allows target nucleic acid sequence detection in pooled biological samples (e.g. pooled blood samples) without prior amplification of the target.Type: ApplicationFiled: May 9, 2002Publication date: August 14, 2003Inventors: Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Monika de Arruda Indig, Robert Roeven
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Publication number: 20030143535Abstract: The present invention relates to methods and compositions for analyzing nucleic acids, and in particular, methods and compositions for detection and characterization of nucleic acid sequences and sequence changes. The present invention also provides methods and compositions for identifying oligonucleotides with desired hybridization properties to nucleic acid targets containing secondary structure.Type: ApplicationFiled: June 15, 2001Publication date: July 31, 2003Applicant: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Hatim Allawi, Fang Dong, Bruce P. Neri, Tatiani I. Vener
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Publication number: 20030143585Abstract: The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.Type: ApplicationFiled: October 11, 2002Publication date: July 31, 2003Applicant: Northwestern UniversityInventors: Priscilla Wilkins Stevens, David Kelso, Victor Lyamichev, Jeff Hall, Bruce P. Neri, Robert W. Kwiatkowski, Lloyd M. Smith
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Publication number: 20030134349Abstract: The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.Type: ApplicationFiled: January 11, 2001Publication date: July 17, 2003Applicant: Third Wave Technologies, Inc.Inventors: Wu-Po Ma, Victor I. Lyamichev, Michael W. Kaiser, Natalie E. Lyamicheva, Hatim Taysir Allawi, James J. Schaefer, Bruce P. Neri
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Publication number: 20030119020Abstract: The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.Type: ApplicationFiled: July 17, 2002Publication date: June 26, 2003Inventors: Priscilla Wilkins Stevens, David Kelso, Victor Lyamichev, Jeff Hall, Bruce P. Neri, Robert W. Kwiatkowski, Lloyd Smith
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Publication number: 20030044796Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on dendrimers and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: August 27, 2001Publication date: March 6, 2003Inventors: Bruce P. Neri, Jeff G. Hall, Victor Lyamichev, Lloyd M. Smith
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Publication number: 20020128465Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.Type: ApplicationFiled: February 6, 2001Publication date: September 12, 2002Applicant: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Zbigniew Skrzpczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Takova, Bruce P. Neri
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Publication number: 20020119454Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: ApplicationFiled: April 3, 2001Publication date: August 29, 2002Inventors: Victor I. Lyamichev, Fang Dong, Mary Ann D. Brow, Lance Fors, Bruce P. Neri
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Patent number: 6210880Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: September 19, 1997Date of Patent: April 3, 2001Assignee: Third Wave Technologies, Inc.Inventors: Victor I. Lyamichev, Fang Dong, Mary Ann D. Brow, Lance Fors, Bruce P. Neri
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Patent number: 5714380Abstract: The present invention features a vessel for isolating a target in a sample. The vessel includes at least one reaction chamber, a wash system and an effluent system. The reaction chamber includes a closed cell adapted to receive a support, a sample potentially containing target and at least one first probe, and thereafter being closed. The probe is capable of associating with the support and the target to form a support-probe-target complex and sample debris upon imposition of probe binding conditions within the reaction chamber. A wash system is capable of introducing solutions into the reaction chamber for washing the support to solubilize and suspend sample debris. Upon imposition of wash conditions, solutions are allowed to enter the reaction chamber to solubilize such sample debris. An Effluent system is in communication with the reaction chamber and capable of receiving sample debris and wash solutions.Type: GrantFiled: March 25, 1996Date of Patent: February 3, 1998Assignee: Amoco CorporationInventors: Bruce P. Neri, John S. Curtis, Mark L. Collins, Danahey Ryan
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Patent number: 5145784Abstract: A capillary flow device useful in double capture assays, such as double capture immunoassays, and a double capture assay. The capillary flow device comprises a capillary track, a sample receptacle, a particle collecting means and, optionally, a magnet and a particle concentrator. The assay method is useful for determining any analyte of interest for which there is a specific binding partner.Type: GrantFiled: January 2, 1992Date of Patent: September 8, 1992Assignee: Cambridge Biotech CorporationInventors: Daniel E. Cox, Obsidiana Abril, Sara Bauminger, Bruce P. Neri, Lisa Shinefeld
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Patent number: 4448889Abstract: In a method for colorimetric determination of calcium, the sample to be analyzed is mixed with a dye reagent of pH value at which that reagent does not produce a predetermined change when complexed with calcium ions; a first colorimetric measurement is made on the mixture of the sample and dye reagent; a second reagent is added to the mixture of the sample and dye reagent to shift the pH and activate the dye reagent producing the predetermined change; and then a second colorimetric measurement is made on the resulting mixture, the difference between the two colorimetric measurements being a measure of calcium in the sample.Type: GrantFiled: June 18, 1982Date of Patent: May 15, 1984Assignee: Instrumentation Laboratory Inc.Inventors: Bruce P. Neri, Stanley M. Liffmann, Carolyn Bergkuist
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Patent number: 4447544Abstract: An aqueous reagent for determining inorganic phosphate in a protein-containing liquid biological sample comprises a molybdate salt, an acid capable of reacting with the molybdate salt to form molybdic acid for complexation with phosphate to form phosphomolybdate complexes, a ferric salt in an amount sufficient to inhibit turbidity in the sample, and a nonionic surfactant reagent in an amount sufficient to further inhibit turbidity in the sample, the molybdate salt and the acid being present in amounts sufficient to form an amount of molybdic acid sufficient to produce a detectible color change when complexed with phosphate in the sample.Type: GrantFiled: July 22, 1982Date of Patent: May 8, 1984Assignee: Instrumentation Laboratory Inc.Inventors: Bruce P. Neri, Stanley M. Liffmann