Patents by Inventor Carrie Graham
Carrie Graham has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10731199Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.Type: GrantFiled: November 20, 2012Date of Patent: August 4, 2020Assignee: ADVANCED LIQUID LOGIC, INC.Inventors: Carrie A. Graham, Allen E. Eckhardt
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Publication number: 20170241949Abstract: A droplet actuator for manipulating a fluid using an electrical field includes a droplet arranged on or over an electrode. The droplet includes a set of beads arranged substantially in a monolayer on or over a surface of the droplet actuator.Type: ApplicationFiled: March 28, 2017Publication date: August 24, 2017Inventors: Donovan Bort, Carrie Graham, Vamsee Pamula, Michael Pollack, Ramakrishna Sista, Vijay Srinivasan
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Publication number: 20170159105Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.Type: ApplicationFiled: February 20, 2017Publication date: June 8, 2017Applicant: Advanced Liquid Logic, Inc.Inventors: Allen E. Eckhardt, Carrie Graham, Ramakrishna Sista, Vamsee K. Pamula, Theodore Winger, Tong Wang, Yalin Xiong, Ning Wu, Sanjay Saha, Gajendrasinh Raolji, Raveendra Dayam
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Patent number: 9574220Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.Type: GrantFiled: March 11, 2015Date of Patent: February 21, 2017Assignee: Advanced Liquid Logic, Inc.Inventors: Allen E Eckhardt, Carrie Graham, Ramakrishna Sista, Vamsee K. Pamula, Theodore Winger, Tong Wang, Yalin Xiong, Ning Wu, Sanjay Saha, Gajendrasinh Raolji, Raveendra Dayam
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Publication number: 20150267242Abstract: A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme-specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals.Type: ApplicationFiled: October 30, 2013Publication date: September 24, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Nora J. Foegeding, Carrie A. Graham, Allen E. Eckhardt
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Patent number: 9140635Abstract: Methods of conducting an assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity are provided. The methods include concentrating the glycoprotein having enzymatic activity by capturing the glycoproteins from the input sample on a solid support, washing the glycoproteins captured on a solid support to remove unbound portions of the input sample, adding an enzyme substrate to the output sample comprising glycoproteins having enzymatic activity, and measuring enzymatic modification of the substrate. In some examples, the capturing of the glycoprotein and subsequent washing steps are performed in one or more droplets in oil.Type: GrantFiled: May 9, 2012Date of Patent: September 22, 2015Assignee: Advanced Liquid Logic, Inc.Inventors: Carrie Graham, Allen Eckhardt, Lisa Perkins
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Publication number: 20150184219Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.Type: ApplicationFiled: March 11, 2015Publication date: July 2, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Allen E Eckhardt, Carrie Graham, Ramakrishna Sista, Vamsee K. Pamula, Theodore Winger, Tong Wang, Yalin Xiong, Ning Wu, Sanjay Saha, Gajendrasinh Raolji, Raveendra Dayam
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Publication number: 20150111237Abstract: The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing.Type: ApplicationFiled: May 7, 2013Publication date: April 23, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Carrie A. Graham, Allen E. Eckhardt
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Publication number: 20150050664Abstract: A method of preparing a sample for conducting an assay, the method including: providing an input sample including glycoproteins; capturing glycoproteins from the input sample on a solid support; and washing the sample support to remove unbound portions of the input sample.Type: ApplicationFiled: May 9, 2012Publication date: February 19, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Carrie Graham, Allen Eckhardt, Lisa Perkins
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Publication number: 20140335069Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.Type: ApplicationFiled: November 20, 2012Publication date: November 13, 2014Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Carrie A. Graham, Allen E. Eckhardt
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Publication number: 20140174926Abstract: A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape.Type: ApplicationFiled: May 1, 2012Publication date: June 26, 2014Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Donovan Bort, Carrie Graham, Vamsee Pamula, Michael Pollack, Ramakrishna Sista, Vijay Srinivasan
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Publication number: 20110118132Abstract: The invention is directed to droplet actuator devices and assay methods. The method may include, among other things, a method of conducting enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil, the method including incubating a droplet in oil, the droplet preferably comprising an umbelliferone substrate, a sample, and a cyclodextrin compound. The methods may further include a method of substantially eliminating cross-contamination between droplets in enzymatic containing substrate-based bioassays. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the inclusion complex may be formed using cyclodextrins. In yet another embodiment, the invention may provide a method of enhancing hydrolysis of enzymatic substrates.Type: ApplicationFiled: January 25, 2011Publication date: May 19, 2011Applicant: Advanced Liquid Logic, Inc.Inventors: Theodore Winger, Allen E. Eckhardt, Carrie Graham, Ramakrishna Sista, Vamsee K. Pamula, Tong Wang, Yalin Xiong, Ning Wu, Sanjay Saha, Gajendrasinh Balvantsinh Raolji, Raveendra Dayam
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Publication number: 20110104725Abstract: The invention provides techniques for coagulating blood on a droplet actuator. The invention also provides methods of manipulating the coagulated blood including a variety of droplet operations that may be conducted using the coagulated blood. Further, the invention provides a variety of assays that make use of the coagulated blood or various blood samples as input.Type: ApplicationFiled: May 4, 2009Publication date: May 5, 2011Applicants: ADVANCED LIQUID LOGIC, INC., CHILDREN'S MEDICAL CENTER CORPORATIONInventors: Vamsee K. Pamula, Sitaram Emani, Allen Eckhardt, Carrie Graham, Jeremy Rouse