Abstract: Novel methods for producing, and compositions of humanized immunoglobulins having one or more complementarity determining regions (CDR's) and possible additional amino acids from a donor immunoglobulin and a framework region from an accepting human immunoglobulin are provided. Each humanized immunoglobulin chain will usually comprise, in addition to the CDR's, amino acids from the donor immunoglobulin framework that are, e.g., capable of interacting with the CDR's to effect binding affinity, such as one or more amino acids which are immediately adjacent to a CDR in the donor immunoglobulin or those within about 3 .ANG. as predicted by molecular modeling. The heavy and light chains may each be designed by using any one or all of various position criteria.

Abstract: Novel methods for producing, and compositions of, humanized immunoglobulins having one or more complementarity determining regions (CDR's) and possible additional amino acids from a donor immunoglobulin and a framework region from an accepting human immunoglobulin are provided. Each humanized immunoglobulin chain will usually comprise, in addition to the CDR's, amino acids from the donor immunoglobulin framework that are, e.g., capable of interacting with the CDR's to effect binding affinity, such as one or more amino acids which are immediately adjacent to a CDR in the donor immunoglobulin or those within about 3 .ANG. as predicted by molecular modeling. The heavy and light chains may each be designed by using any one or all of various position criteria.

Abstract: Improved means for producing Clostridium Phospholipase C (PL) polypeptides based on the cloning and expression of recombinant DNA segments containing Clostridium PLC genes and fragments. The DNA segments are operably linked to host specific expression control sequences for exogenous production of Clostridium PLC, or fragments thereof, substantially free from naturally-associated Clostridium gene products.

Abstract: Method and apparatus for comparing original and modified versions of a document. The system of the present invention utilizes a hash number generator CPU to generate hash numbers for lines and sentences contained in the documents. Matching hash numbers are defined as anchorpoints and stored in an anchorpoint memory. A comparator CPU performs a character-by-character comparison of the respective documents radiating outward from each anchorpoint. This comparison generates identity blocks which are defined as blocks which are the same in both documents. Non-identity blocks are defined as difference blocks and are characterized as insertions or deletions depending on their status. A portion of the original and modified document is displayed in a split-screen format on a display, such as a CRT. Cursors on the top and bottom half of the screen identify corresponding portions of the documents.