Patents by Inventor Casey J. Krusemark

Casey J. Krusemark has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210284691
    Abstract: The present invention relates to series of peptidomimetic compounds selectively targeting CBX8 of polycomb chromobox protein homolog proteins. Pharmaceutical compositions of those compounds and methods of using them in the treatment of diseases involved CBX8 pharmacology, including various cancers and leukemia, by administering therapeutically effective amounts of such compound alone or together with other therapeutics, are within the scope of this disclosure.
    Type: Application
    Filed: May 18, 2021
    Publication date: September 16, 2021
    Applicant: Purdue Research Foundation
    Inventors: Casey J Krusemark, Emily C Dykhuizen, Sijie Wang
  • Publication number: 20210269863
    Abstract: Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.
    Type: Application
    Filed: May 18, 2021
    Publication date: September 2, 2021
    Applicant: Purdue Research Foundation
    Inventors: Casey J. Krusemark, Kyle Denton, Dongwook Kim, Rachael Jetson
  • Patent number: 11028427
    Abstract: Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.
    Type: Grant
    Filed: August 18, 2016
    Date of Patent: June 8, 2021
    Assignee: Purdue Research Foundation
    Inventors: Casey J. Krusemark, Kyle E. Denton, Dongwook Kim, Rachael R. Jetson
  • Publication number: 20200385424
    Abstract: The present invention relates to series of peptidomimetic compounds selectively targeting CBX8 of polycomb chromobox protein homolog proteins. Pharmaceutical compositions of those compounds and methods of using them in the treatment of diseases involved CBX8 pharmacology, including various cancers and leukemia, by administering therapeutically effective amounts of such compound alone or together with other therapeutics, are within the scope of this disclosure.
    Type: Application
    Filed: June 5, 2020
    Publication date: December 10, 2020
    Applicant: Purdue Research Foundation
    Inventors: Casey J. Krusemark, Emily C. Dykhuizen, Sijie Wang
  • Publication number: 20180237831
    Abstract: Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.
    Type: Application
    Filed: August 18, 2016
    Publication date: August 23, 2018
    Applicant: Purdue Research Foundation
    Inventors: Casey J. Krusemark, Kyle E. Denton, Dongwook Kim, Rachael R. Jetson
  • Patent number: 8592216
    Abstract: The present invention provides methods for enhancing the fragmentation of peptides for mass spectrometry by modifying the peptides with a tagging reagent containing a functional group, such as a tertiary amine, having a greater gas-phase basicity than the amide backbone of the peptide. These high gas-phase basicity functional groups are attached to a peptide by reacting the tagging reagent to one or more available carboxylic acid groups of the peptide. Linking these high gas-phase functional groups to the peptides leads to higher charge state ions from electrospray ionization mass spectrometry (ESI-MS), which fragment more extensively during fragmentation techniques, particularly non-ergodic fragmentation techniques such as electron capture dissociation (ECD) and electron transfer dissociation (ETD).
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: November 26, 2013
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brian L. Frey, April L. Jue, Casey J. Krusemark, Lloyd M. Smith, Joshua J. Coon
  • Patent number: 8563777
    Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: October 22, 2013
    Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the University of Illinois
    Inventors: Lloyd M. Smith, Michael R. Shortreed, Brian L. Frey, Margaret F. Phillips, Joshua J. Coon, Shane M. Lamos, Casey J. Krusemark, Peter J. Belshaw, Madhusudan Patel, Neil L. Kelleher
  • Publication number: 20120022230
    Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.
    Type: Application
    Filed: June 8, 2011
    Publication date: January 26, 2012
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Lloyd M. SMITH, MICHAEL R. SHORTREED, BRIAN L. FREY, MARGARET F. PHILLIPS, JOSHUA J. COON, SHANE M. LAMOS, CASEY J. KRUSEMARK, PETER J. BELSHAW, MADHUSUDAN PATEL, NEIL L. KELLEHER
  • Patent number: 7982070
    Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic kit facilitating relative quantification and providing tangible evidence for the existence of specific functional groups. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules, such as biological samples. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. Advantageously, the labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.
    Type: Grant
    Filed: March 21, 2007
    Date of Patent: July 19, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Lloyd M. Smith, Michael R. Shortreed, Brian L. Frey, Margaret F. Phillips, Joshua J. Coon, Shane M. Lamos, Casey J. Krusemark, Peter J. Belshaw, Madhusudan Patel, Neil L. Kelleher
  • Publication number: 20100330680
    Abstract: The present invention provides methods for enhancing the fragmentation of peptides for mass spectrometry by modifying the peptides with a tagging reagent containing a functional group, such as a tertiary amine, having a greater gas-phase basicity than the amide backbone of the peptide. These high gas-phase basicity functional groups are attached to a peptide by reacting the tagging reagent to one or more available carboxylic acid groups of the peptide. Linking these high gas-phase functional groups to the peptides leads to higher charge state ions from electrospray ionization mass spectrometry (ESI-MS), which fragment more extensively during fragmentation techniques, particularly non-ergodic fragmentation techniques such as electron capture dissociation (ECD) and electron transfer dissociation (ETD).
    Type: Application
    Filed: April 14, 2010
    Publication date: December 30, 2010
    Inventors: Brian L. Frey, April L. Jue, Casey J. Krusemark, Lloyd M. Smith, Joshua J. Coon