Patents by Inventor Chang-Guo Zhan

Chang-Guo Zhan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20110168636
    Abstract: Methods are described for removing aluminum from a solution using novel di- and tripodal compounds as chelators.
    Type: Application
    Filed: March 21, 2011
    Publication date: July 14, 2011
    Inventors: Robert A. Yokel, Wesley R. Harris, Christopher D. Spilling, Chang-Guo Zhan
  • Patent number: 7932326
    Abstract: Novel di- and tripodal compounds useful as chelators, intermediates for their production and a method for treating an aqueous solution to remove trivalent metal ions are presented.
    Type: Grant
    Filed: April 16, 2008
    Date of Patent: April 26, 2011
    Assignee: The University of Kentucky Research Foundation
    Inventors: Robert A. Yokel, Wesley R. Harris, Christopher D. Spilling, Chang-Guo Zhan
  • Patent number: 7919082
    Abstract: A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, A199S/F227A/S287G/A328W/Y332G, and A199S/F227A/S287G/A328W/E441D all have enhanced catalytic efficiency for (?)-cocaine compared with wild-type BChE.
    Type: Grant
    Filed: April 1, 2010
    Date of Patent: April 5, 2011
    Assignee: University of Kentucky Research Foundation
    Inventors: Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai
  • Patent number: 7892537
    Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.
    Type: Grant
    Filed: April 26, 2010
    Date of Patent: February 22, 2011
    Assignee: University of Kentucky Research Foundation
    Inventors: Chang-Guo Zhan, Fang Zheng, Wenchao Yang
  • Patent number: 7740840
    Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.
    Type: Grant
    Filed: January 11, 2010
    Date of Patent: June 22, 2010
    Assignee: University of Kentucky Research Foundation
    Inventors: Chang-Guo Zhan, Fang Zheng, Wenchao Yang
  • Patent number: 7731957
    Abstract: A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, A199S/F227A/S287G/A328W/Y332G, and A199S/F227A/S287G/A328W/E441D all have enhanced catalytic efficiency for (?)-cocaine compared with wild-type BChE.
    Type: Grant
    Filed: August 15, 2008
    Date of Patent: June 8, 2010
    Assignee: University of Kentucky Research Foundation
    Inventors: Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai
  • Publication number: 20100034799
    Abstract: Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.
    Type: Application
    Filed: July 10, 2007
    Publication date: February 11, 2010
    Inventors: Donald Landry, Chang-Guo Zhan, James H. Woods, Roger Sunahara, Diwahar L. Narasimhan, Joanne MacDonald, Victor Yang, Mei-Chuan Holden Ko, Shi-Xian Deng, John J. Tesmer, Tien-Yi Lee, Young Min Kwon, Daquan Gao
  • Publication number: 20090261043
    Abstract: Novel di- and tripodal compounds useful as chelators, intermediates for their production and a method for treating an aqueous solution to remove trivalent metal ions are presented.
    Type: Application
    Filed: April 16, 2008
    Publication date: October 22, 2009
    Inventors: Robert A. Yokel, Wesley R. Harris, Christopher D. Spilling, Chang-Guo Zhan
  • Patent number: 7438904
    Abstract: A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, A199S/F227A/S287G/A328W/Y332G, and A199S/F227A/S287G/A328W/E441D all have enhanced catalytic efficiency for (?)-cocaine compared with wild-type BChE.
    Type: Grant
    Filed: October 4, 2005
    Date of Patent: October 21, 2008
    Assignee: University of Kentucky Research Foundation
    Inventors: Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai
  • Publication number: 20080177521
    Abstract: This invention relates to representations of prostaglandin synthase three-dimensional structures. Such representations are suitable for designing agents that modulate the activity of the enzyme by binding to the substrate binding domain.
    Type: Application
    Filed: January 24, 2007
    Publication date: July 24, 2008
    Applicant: The University of Kentucky
    Inventors: Chang-Guo Zhan, Xiaoqin Huang, Adel Hamza