Patents by Inventor Chihiro Uematsu

Chihiro Uematsu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10793821
    Abstract: The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).
    Type: Grant
    Filed: January 22, 2016
    Date of Patent: October 6, 2020
    Assignee: HITACHI HIGH-TECH CORPORATION
    Inventors: Chihiro Uematsu, Muneo Maeshima
  • Publication number: 20200102587
    Abstract: Provided are a method and apparatus for forming clusters of amplified nucleic acid fragments without amplification bias, in a regular arrangement on a substrate. In the method according to the present invention, droplets enclosing the template nucleic acid are formed on a substrate that has a plurality of first surfaces having hydrophilicity and a second surface surrounding each of the plurality of first surfaces and being less hydrophilic than the first surfaces. Then, after a nucleic acid amplification reaction is performed in the droplets on the substrate, the droplets are removed and a nucleic acid amplification reaction is further performed on the substrate.
    Type: Application
    Filed: April 5, 2017
    Publication date: April 2, 2020
    Inventors: Takahide YOKOI, Chihiro UEMATSU, Naoshi ITABASHI
  • Publication number: 20190292505
    Abstract: A culturing device includes a microplate including a plurality of vessels, each of the vessels having a bottom surface having light transmittance and an opening at an upper portion, a lid having light transmittance, and an intermediate plate having light transmittance sandwiched between the lid and the microplate. The intermediate plate has a plurality of convex portions on a surface thereof facing the microplate and provided with a plurality of through holes corresponding to the convex portions that are disposed so that when the intermediate plate and the microplate are overlapped, each of the plurality of convex portions is inserted into each of the plurality of vessels and each of the plurality of through holes coincides with the opening of each of the plurality of vessels. The lid comes into contact with the intermediate plate so as to close the plurality of through holes provided in the intermediate plate.
    Type: Application
    Filed: December 14, 2016
    Publication date: September 26, 2019
    Inventors: Chihiro UEMATSU, Hiroko FUJITA, Akira MASUYA
  • Patent number: 10323266
    Abstract: Provided is a antimicrobial susceptibility testing device, including: an ATP examination culture plate that includes a reaction vessel, a reagent holding parts for holding reagents to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has plural layers that can be joined and separated; a gas feeding path for feeding a gas into the ATP examination culture plate; a heater; an optical detection unit; and a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on a detection result of the optical detection unit, wherein when the plural layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.
    Type: Grant
    Filed: March 17, 2015
    Date of Patent: June 18, 2019
    Assignee: HITACHI, LTD.
    Inventors: Hideyuki Noda, Hiroko Tada, Chihiro Uematsu, Masahiro Okanojo
  • Publication number: 20180195959
    Abstract: A light detection device comprises: a flow path section in which a plurality of lines of flow of a plurality of droplets 4 dispersed in oil and moving along a linear line of flow is retained on an array plane; a laser beam irradiation section which introduces a laser beam 3 in a direction in which the plurality of lines of flow are arrayed in the flow path section to irradiate the plurality of lines of flow; and a light detection section which detects emitted light generated from the plurality of lines of flow by the irradiation of the laser beam, from a vertical direction with respect to the array plane. A refractive index of the oil no and a refractive index of the droplets nd have a difference such that ?0.02?nd?no?0.05.
    Type: Application
    Filed: August 21, 2015
    Publication date: July 12, 2018
    Applicant: Hitachi, Ltd.
    Inventors: Takashi ANAZAWA, Yoshinobu KOHARA, Chihiro UEMATSU, Yusuke GOTO
  • Publication number: 20180057853
    Abstract: Provided is a antimicrobial susceptibility testing device, including: an ATP examination culture plate that includes a reaction vessel, a reagent holding parts for holding reagents to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has plural layers that can be joined and separated; a gas feeding path for feeding a gas into the ATP examination culture plate; a heater; an optical detection unit; and a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on a detection result of the optical detection unit, wherein when the plural layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.
    Type: Application
    Filed: March 17, 2015
    Publication date: March 1, 2018
    Inventors: Hideyuki NODA, Hiroko TADA, Chihiro UEMATSU, Masahiro OKANOJO
  • Publication number: 20180010084
    Abstract: The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).
    Type: Application
    Filed: January 22, 2016
    Publication date: January 11, 2018
    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Chihiro UEMATSU, Muneo MAESHIMA
  • Patent number: 9714449
    Abstract: A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA (1, 2) containing a target DNA sequence (1) to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (5). A 3?-end elongation reaction is performed using a strand-displacement DNA polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target DNA sequences (1) and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA. The concatemer (29) contains a plurality of the target DNA sequences (1) suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.
    Type: Grant
    Filed: November 22, 2012
    Date of Patent: July 25, 2017
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yoshiaki Yazawa, Takahide Yokoi, Chihiro Uematsu
  • Publication number: 20170096631
    Abstract: Provided is a test apparatus in which a test for bacterial identification or antimicrobial susceptibility can be promptly determined. A division state of bacteria is monitored by performing microscopic observation of shapes and the number of the bacteria in each of wells in a culture plate for bacterial identification culture or an antimicrobial susceptibility test, and it is determined whether or not the bacteria grow in a stage shifted from an induction phase to a logarithmic phase, with reference to an image obtained through microscopic observation. In addition, determination performed based on turbidity in the related art may be combined with determination performed based on microscopic observation in which change and the like in the shapes of the bacteria are monitored. Accordingly, it is possible to realize a highly accurate test result.
    Type: Application
    Filed: September 19, 2014
    Publication date: April 6, 2017
    Inventors: Chihiro UEMATSU, Muneo MAESHIMA, Akira MASUYA
  • Patent number: 9523611
    Abstract: A surface temperature measuring apparatus comprising a radiation thermometer configured to detect thermal radiation light emitted from a surface of a temperature measurement target material in a cooling process with water, a housing having an opening on a temperature measurement target material side, the housing storing, in an inside of the housing, at least a light receiving unit of the radiation thermometer among structural elements of the radiation thermometer, and an optical glass that is fit and sealed in the inside of the housing between the temperature measurement target material and the light receiving unit of the radiation thermometer, the optical glass being configured to transmit the thermal radiation light. The optical glass has, on the temperature measurement target material side, an end surface adjacent to the surface of the temperature measurement target material. A surface temperature measuring method is also provided.
    Type: Grant
    Filed: August 21, 2013
    Date of Patent: December 20, 2016
    Assignee: Nippon Steel & Sumitomo Metal Corporation
    Inventors: Chihiro Uematsu, Yutaka Wakasu, Tatsuro Honda
  • Patent number: 9442127
    Abstract: This invention concerns a sample processing device capable of efficiently recovering biological molecules, such as nucleic acids or proteins. The sample processing device is capable of placing a reaction container having a plurality of reaction sites, and it comprises a nozzle mechanism with a nozzle capable of attaching and removing a dispenser tip for dispensing a solution into the reaction sites of the reaction container and a magnetic tip for generating a magnetic field that allows magnetic beads to migrate to a space among the plurality of reaction sites in the reaction container, and a drive control unit controlling the nozzle mechanism.
    Type: Grant
    Filed: December 8, 2010
    Date of Patent: September 13, 2016
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Chihiro Uematsu, Muneo Maeshima
  • Patent number: 9221055
    Abstract: Nucleic acid analysis apparatus includes a plurality of temperature adjustment apparatuses, a rotating mechanism, a delivery base and an ejection base, a delivery drive mechanism, an ejection drive mechanism, and a detection apparatus. The rotating mechanism can include a rotating shaft and a plurality of pressing portions that rotate around the rotating shaft. The reaction plate assembly can move over the temperature adjustment apparatuses along the circumferential direction in a state of being pressed onto the temperature adjustment apparatuses by the pressing portions. The delivery drive mechanism can cause the reaction plate assembly to be moved radially inward and delivered between the pressing portions and temperature adjustment apparatuses. The ejection drive mechanism can cause the reaction plate assembly to be moved radially outward and ejected from between the pressing portions and temperature adjustment apparatuses onto the ejection base.
    Type: Grant
    Filed: October 27, 2011
    Date of Patent: December 29, 2015
    Assignee: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Yasunori Shoji, Muneo Maeshima, Chihiro Uematsu, Makiko Takahashi, Kyoko Imai
  • Patent number: 9188493
    Abstract: A main object of the present invention is to provide a method capable of measuring surface temperatures of a plurality of points of cast slab at a secondary cooling zone of a continuous caster with a good accuracy and at low cost. The method comprises the steps of inserting one end of a plurality of optical fibers 3 respectively to nozzles 1 and tubes 2, and installing each nozzle between support rolls that support the cast slab, while spraying purge air from each nozzle toward a surface of the cast slab, receiving a thermal radiation light from the cast slab at the one end of each optical fiber, gathering other ends of the optical fibers 3, 81 in a bundle to take images including a core image of the other end of each optical fibers by an imaging means 5, and calculating the surface temperature of the cast slab.
    Type: Grant
    Filed: August 27, 2013
    Date of Patent: November 17, 2015
    Assignee: NIPPON STEEL & SUMITOMO METAL CORPORATION
    Inventors: Tatsuro Honda, Chihiro Uematsu, Yoichi Inoue, Naoki Tajima, Yasuhiro Mizuno
  • Publication number: 20150226610
    Abstract: To provide a surface temperature measuring apparatus that is capable of accurately measuring a surface temperature of a temperature measurement target material such as a steel material (e.g., a wheel, a steel pipe, a steel sheet, or a rail) in a cooling process with water, and a method of measuring the same.
    Type: Application
    Filed: August 21, 2013
    Publication date: August 13, 2015
    Applicant: NIPPON STEEL & SUMITOMO METAL CORPORATION
    Inventors: Chihiro Uematsu, Yutaka Wakasu, Tatsuro Honda
  • Publication number: 20150204732
    Abstract: A main object of the present invention is to provide a method capable of measuring surface temperatures of a plurality of points of cast slab at a secondary cooling zone of a continuous caster with a good accuracy and at low cost. The method comprises the steps of inserting one end of a plurality of optical fibers 3 respectively to nozzles 1 and tubes 2, and installing each nozzle between support rolls that support the cast slab, while spraying purge air from each nozzle toward a surface of the cast slab, receiving a thermal radiation light from the cast slab at the one end of each optical fiber, gathering other ends of the optical fibers 3, 81 in a bundle to take images including a core image of the other end of each optical fibers by an imaging means 5, and calculating the surface temperature of the cast slab.
    Type: Application
    Filed: August 27, 2013
    Publication date: July 23, 2015
    Inventors: Tatsuro Honda, Chihiro Uematsu, Yoichi Inoue, Noaki Tajima, Yasuhiro Mizuno
  • Patent number: 8895246
    Abstract: This invention provides a novel method for amplifying and detecting a target gene rapidly with high sensitivity under isothermal conditions. In such method, a sequence to be amplified can be freely designed regardless of the template sequence, an amplified product can be amplified and detected within a short period of time with high sensitivity, and thus, the gene expression level can be determined more easily than is possible with prior art.
    Type: Grant
    Filed: January 17, 2006
    Date of Patent: November 25, 2014
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Maiko Tanabe, Chihiro Uematsu
  • Publication number: 20140342922
    Abstract: A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA (1, 2) containing a target DNA sequence (1) to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (5). A 3?-end elongation reaction is performed using a strand-displacement DNA polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target DNA sequences (1) and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA. The concatemer (29) contains a plurality of the target DNA sequences (1) suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.
    Type: Application
    Filed: November 22, 2012
    Publication date: November 20, 2014
    Inventors: Yoshiaki Yazawa, Takahide Yokoi, Chihiro Uematsu
  • Publication number: 20130230908
    Abstract: Provided is a technology such that, in nucleic acid analysis, a high degree of freedom in loading or unloading a reaction plate can be obtained and a sample can be efficiently analyzed. A reaction plate assembly includes a reaction plate with one or more reaction wells, a visible light transmissive cover mounted on the reaction plate and covering the reaction wells, and a visible light transmissive weight member covering the cover. The reaction wells are disposed in an arc shape along the circumference of a circle with a predetermined radius r1.
    Type: Application
    Filed: October 27, 2011
    Publication date: September 5, 2013
    Inventors: Yasunori Shoji, Muneo Maeshima, Chihiro Uematsu, Makiko Takahashi, Kyoko Imai
  • Publication number: 20120309104
    Abstract: This invention concerns a sample processing device capable of efficiently recovering biological molecules, such as nucleic acids or proteins. The sample processing device is capable of placing a reaction container having a plurality of reaction sites, and it comprises a nozzle mechanism with a nozzle capable of attaching and removing a dispenser tip for dispensing a solution into the reaction sites of the reaction container and a magnetic tip for generating a magnetic field that allows magnetic beads to migrate to a space among the plurality of reaction sites in the reaction container, and a drive control unit controlling the nozzle mechanism.
    Type: Application
    Filed: December 8, 2010
    Publication date: December 6, 2012
    Applicant: Hitachi High-Technologies Corporation
    Inventors: Chihiro Uematsu, Muneo Maeshima
  • Patent number: 8008045
    Abstract: The present application relates to primers for isothermal amplification of HCV each include at least eighteen consecutive bases corresponding to a 3? end region of one selected from base sequences of SEQ ID NOs: 1-10, 21 and 22. The primers are specific to HCV subtypes 1a, 1b, 2a, 2b and 3a, respectively and enable genotyping of HCV by isothermal amplification.
    Type: Grant
    Filed: October 23, 2007
    Date of Patent: August 30, 2011
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Maiko Tanabe, Chihiro Uematsu