Patents by Inventor Chihiro Uematsu
Chihiro Uematsu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20080318282Abstract: Provided is a simple and highly sensitive nucleic acid amplification method including hybridizing two types of oligonucleotide probes with a target gene and ligating the oligonucleotide probes with DNA ligase and amplifying the resultant single-stranded oligonucleotide in accordance with LAMP.Type: ApplicationFiled: May 30, 2008Publication date: December 25, 2008Inventors: Chihiro Uematsu, Yukie Nakashima, Toshiyuki Hatano
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Patent number: 7439522Abstract: Single molecule measurement is conducted by propagating a laser light under multiple reflection between two substrates constituting a flow channel for flowing a sample solution, exciting target molecules in the sample, detecting one-dimensional images of generated fluorescence by a one-dimensional detection portion, synthesizing two-dimensional images from the obtained one-dimensional images by a data synthesizing portion and measuring the concentration of target molecules in the sample solution by counting the number of target molecules based on the two-dimensional images, thereby measuring the concentration of the target molecules in the sample solution. These constitutions for single molecule measurement lead to high precision determination of a micro-amount of a biological material.Type: GrantFiled: February 9, 2006Date of Patent: October 21, 2008Assignee: Hitachi High-Technologies CorporationInventors: Masataka Shirai, Tsuyoshi Sonehara, Takashi Anazawa, Chifumi Gouda, Chihiro Uematsu
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Publication number: 20070238125Abstract: The present invention relates to a probe synthesis method and a probe synthesis kit for nucleic acid detection, which are intended for the fluorophore labeling and detection of a target gene. In this method, a probe unit for analyte nucleic acid recognition having a base sequence specific to an analyte nucleic acid and plural labeled probe units each having a fluorophore labeled internal base are hybridized to oligonucleotides complementary thereto, and the adjacent probe units are bonded by ligation. As a result, an analytical probe with increased fluorescence intensity is synthesized easily and inexpensively, while the number of fluorophore labels is controlled.Type: ApplicationFiled: April 6, 2007Publication date: October 11, 2007Inventors: Chihiro Uematsu, Chifumi Gouda, Masataka Shirai, Takashi Anazawa
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Publication number: 20070231808Abstract: This invention provides a method of nucleic acid analysis that enables highly accurate and sensitive quantitation by counting the number of molecules among a plurality of types of genes without amplifying specific genes and that enable reduction of quantitation limits.Type: ApplicationFiled: May 26, 2006Publication date: October 4, 2007Inventors: Chifumi Gouda, Chihiro Uematsu, Takashi Anazawa, Masataka Shirai
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Publication number: 20070215817Abstract: Single molecular measurement is conducted with a high throughput. Fluorescence from labeled target molecules flowing a sample flow cell is measured by a line CCD element to realize single molecular measurement with a high throughput. Where, the number of photo detecting pixels of the line CCD element is smaller than a value obtained by dividing an exposure time by pixel transfer rate.Type: ApplicationFiled: February 13, 2007Publication date: September 20, 2007Inventors: Masataka Shirai, Takashi Anazawa, Chihiro Uematsu, Chifumi Gouda
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Patent number: 7241597Abstract: The inventive method for assaying DNA fragments in mixture comprises step 1 of ligating different oligomers hybridizable to primers of the same melting temperature and the same length to individual groups of DNA fragments in a set of DNA fragments; step 2 of mixing together the groups of DNA fragments ligated with the oligomers; step 3 of simultaneous PCR of the groups of DNA fragments ligated with the oligomers in one receptacle by using the primers being complementary to the oligomers and corresponding to the individual groups; and step 4 of detecting PCR amplified DNA fragments; characterized in that the method enables the comparison of plural samples under no influence of PCR reproducibility.Type: GrantFiled: August 6, 2003Date of Patent: July 10, 2007Assignee: Hitachi, Ltd.Inventors: Chihiro Uematsu, Hideki Kambara, Kazunon Okano
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Publication number: 20060223085Abstract: This invention provides a novel method for amplifying and detecting a target gene rapidly with high sensitivity under isothermal conditions. In such method, a sequence to be amplified can be freely designed regardless of the template sequence, an amplified product can be amplified and detected within a short period of time with high sensitivity, and thus, the gene expression level can be determined more easily than is possible with prior art.Type: ApplicationFiled: January 17, 2006Publication date: October 5, 2006Inventors: Maiko Tanabe, Chihiro Uematsu
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Publication number: 20060197034Abstract: Single molecule measurement is conducted by propagating a laser light under multiple reflection between two substrates constituting a flow channel for flowing a sample solution, exciting target molecules in the sample, detecting one-dimensional images of generated fluorescence by a one-dimensional detection portion, synthesizing two-dimensional images from the obtained one-dimensional images by a data synthesizing portion and measuring the concentration of target molecules in the sample solution by counting the number of target molecules based on the two-dimensional images, thereby measuring the concentration of the target molecules in the sample solution. These constitutions for single molecule measurement lead to high precision determination of a micro-amount of a biological material.Type: ApplicationFiled: February 9, 2006Publication date: September 7, 2006Inventors: Masataka Shirai, Tsuyoshi Sonehara, Takashi Anazawa, Chifumi Gouda, Chihiro Uematsu
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Publication number: 20060045809Abstract: A biological sensor utilizing no label, such as a fluorescent material, and that does not require the setting of any marker to a sample. The sensor can make measurement easily and quickly. Molecules to be coupled with an object substance contained in a sample are oscillated, and state changes of the oscillating molecules are measured before and after the coupling, thereby measuring the weight of the object molecules. As a result, the influence of impurities that differ among oscillation states may be suppressed. Periodical signals caused by the oscillation of molecules are subjected to lock-in measurement to reduce the 1/f noise generated by the combination of the object substance with the receptor on the surface of a solid body to improve measurement sensitivity.Type: ApplicationFiled: November 30, 2004Publication date: March 2, 2006Inventors: Masataka Shirai, Toshiki Sugawara, Hiroyuki Uchiyama, Chihiro Uematsu
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Publication number: 20060040261Abstract: The present application relates to primers for isothermal amplification of HCV each include at least eighteen consecutive bases corresponding to a 3? end region of one selected from base sequences of SEQ ID NOs: 1-10, 21 and 22. The primers are specific to HCV subtypes 1a, 1b, 2a, 2b and 3a, respectively and enable genotyping of HCV by isothermal amplification.Type: ApplicationFiled: May 19, 2005Publication date: February 23, 2006Inventors: Maiko Tanabe, Chihiro Uematsu
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Publication number: 20050100910Abstract: The inventive method for assaying DNA fragments in mixture comprises step 1 of ligating different oligomers hybridizable to primers of the same melting temperature and the same length to individual groups of DNA fragments in a set of DNA fragments; step 2 of mixing together the groups of DNA fragments ligated with the oligomers; step 3 of simultaneous PCR of the groups of DNA fragments ligated with the oligomers in one receptacle by using the primers being complementary to the oligomers and corresponding to the individual groups; and step 4 of detecting PCR amplified DNA fragments; characterized in that the method enables the comparison of plural samples under no influence of PCR reproducibility.Type: ApplicationFiled: August 6, 2003Publication date: May 12, 2005Inventors: Chihiro Uematsu, Hideki Kambara, Kazunon Okano
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Publication number: 20040209291Abstract: This invention provides a novel kit for detecting nucleic acid that can be universally used independent of the target nucleic acid sequence, and a simple method for detecting nucleic acid utilizing the same. This method comprises: subjecting a gene to be analyzed to real-time detection using a primer comprising a base sequence specifically hybridizing to the target gene or nucleic acid and the TaqMan® probe or the Molecular Beacon comprising a base sequence identical or complementary to the first base sequence, wherein the gene to be analyzed is prepared by introducing the first base sequence and the second base sequence comprising the T7 promoter sequence, which are nonspecific to the base sequence of the target gene or nucleic acid, into the target gene or nucleic acid so that the second base sequence is bound to a position closer to the 5′ end than the first base sequence. This invention also provides a universal probe for detecting nucleic acid.Type: ApplicationFiled: January 20, 2004Publication date: October 21, 2004Inventors: Chihiro Uematsu, Kazunori Okano
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Patent number: 6773901Abstract: Adenylation of a DNA fragment with a DNA polymerase occurs in the course of PCR, and thus two peaks are detected. To prevent the peak splitting, it is necessary to raise efficiency of adenylation a single peak to occur without changing reaction conditions. To this end, four types of PCR primers which, respectively, have an anchor sequence at 5′ terminus so that any of A, C, G or T is attached to at the 5′ terminus of the anchor sequence, and PCR is carried out by use of the respective primers to determine efficiencies of adenylation. Subsequently, an anchor sequence that is more likely to undergo adenylation is screened to decide an anchor sequence more likely undergo adenylation, followed by PCR by use of a primer having the decided anchor sequence.Type: GrantFiled: February 27, 2002Date of Patent: August 10, 2004Assignee: Hitachi, Ltd.Inventors: Chihiro Uematsu, Kazunori Okano, Takashi Irie
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Publication number: 20040091921Abstract: This invention provides a novel kit for gene expression analysis that can be universally used independent of the target nucleic acid sequence, and a simple method for gene expression analysis utilizing the same. This method comprises: subjecting a gene to be analyzed to real-time PCR detection using a primer comprising a base sequence specifically hybridizing to the target gene, a primer comprising a base sequence identical to the second base sequence, and the TaqMan® probe comprising a base sequence identical or complementary to the first base sequence, wherein the gene to be analyzed is prepared by introducing the first base sequence and the second base sequence, which are nonspecific to the base sequence of the target gene, into the target gene so that the second base sequence is bound to a position closer to the 5′ end than the first base sequence.Type: ApplicationFiled: August 29, 2003Publication date: May 13, 2004Inventors: Chihiro Uematsu, Kazunori Okano
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Publication number: 20030099961Abstract: Adenylation of a DNA fragment with a DNA polymerase occurs in the course of PCR, and thus two peaks are detected. To prevent the peak splitting, it is necessary to raise efficiency of adenulation a single peak to occur without changing reaction conditions. To this end, four types of PCR primers which, respectively, have an anchor sequence at 5′ terminus so that any of A, C, G or T is attached to at the 5′ terminus of the anchor sequence, and PCR is carried out by use of the respective primers to determine efficiencies of adenylation. Subsequently, an anchor sequence that is more likely to undergo adenylation is screened to decide an anchor sequence more likely undergo adenylation, followed by PCR by use of a primer having the decided anchor sequence.Type: ApplicationFiled: February 27, 2002Publication date: May 29, 2003Applicant: Hitachi, Ltd.Inventors: Chihiro Uematsu, Kazunori Okano, Takashi Irie
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Patent number: 6514702Abstract: There are beforehand prepared a monomer having a reaction residue and a polynucleotide probe set comprising plural kinds of polynucleotide probes having a residue bonded to the reaction residue. The monomer is mixed with each kind of polynucleotide probes comprising any plural probes selected from the polynucleotide probe set. Each kind of the resultant mixtures is added to each of different small holes to make the mixture into gel matrix. Thus, a polynucleotide probe chip is produced. Sample DNA is forcibly migrated in the gels by electrophoresis. Laser light is projected onto the side face of the chip. The fluorescence emitted from the whole surface of the chip is collectively detected with a high-sensitive two-dimensional detector. Thus, the polynucleotide probe chip, holding various kinds of DNA probes, for detecting DNA can be provided. This chip has high hybridization-efficiency and makes high-sensitivity and high-speed DNA detection possible.Type: GrantFiled: September 28, 2000Date of Patent: February 4, 2003Assignee: Hitachi, Ltd.Inventors: Kazunori Okano, Hideki Kambara, Chihiro Uematsu, Hiroko Matsunaga, Takashi Irie, Tomoharu Kajiyama, Kenji Yasuda
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Publication number: 20020098490Abstract: The inventive method for assaying DNA fragments in mixture comprisesType: ApplicationFiled: April 27, 2001Publication date: July 25, 2002Applicant: Hitachi, Ltd.Inventors: Chihiro Uematsu, Hideki Kambara, Kazunori Okano
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Patent number: 6242193Abstract: A single molecule of single-stranded sample DNA (7) having a bead (5) at one end and a magnetic bead (6) at the other end is extended and fixed in the field of view of a fluorescent microscope by using a magnetic force (11) and a laser trap (3), and a primer (8) is bonded thereto, followed by elongation reaction (10) using polymerase. Only a single chemically modified nucleotide (9) labeled with at least one fluorophore which varies depending on the kind of the base is incorporated. Only the single fluorophore incorporated is measured as a fluorescence-microscopic image by evanescent irradiation (13) with exciting laser beams, and the kind of the base is determined from the kind of the fluorophore. The fluorophore labeling the nucleotide incorporated is released by evanescent irradiation (13) with ultraviolet laser beams (2), and the next nucleotide is incorporated. DNA sequencing is carried out by repeating the above procedure.Type: GrantFiled: May 9, 2000Date of Patent: June 5, 2001Assignee: Hitachi, Ltd.Inventors: Takashi Anazawa, Kazunori Okano, Chihiro Uematsu, Hideki Kambara
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Patent number: 6225064Abstract: The inventive method for assaying DNA fragments in mixture comprises step 1 of ligating different oligomers hybridizable to primers of the same melting temperature and the same length to individual groups of DNA fragments in a set of DNA fragments; step 2 of mixing together the groups of DNA fragments ligated with the oligomers; step 3 of simultaneous PCR of the groups of DNA fragments ligated with the oligomers in one receptacle by using the primers being complementary to the oligomers and corresponding to the individual groups; and step 4 of detecting PCR amplified DNA fragments; characterized in that the method enables the comparison of plural samples under no influence of PCR reproducibility.Type: GrantFiled: October 7, 1999Date of Patent: May 1, 2001Assignee: Hitachi, Ltd.Inventors: Chihiro Uematsu, Hideki Kambara, Kazunori Okano
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Patent number: 6203988Abstract: A DNA fragment preparation method for DNA analysis comprising, i) preparing a plurality of DNA fragments from a sample DNA, and ii) amplifying a specific DNA fragment by PCR, using a pair of primers which hybridize with terminus sequences of the DNA fragments, and a specific primer which hybridizes specifically with a base sequence of the specific DNA fragment at a position between a priming site of one of primer the pair of primers and a priming site of another primer of the pair of primers. The specific primer hybridizes specifically with a base sequence at a middle position of the specific DNA fragment. Products of PCR are separated, by electrophoresis, and signals from DNA fragments originated in a known genes and signals from DNA fragments originated in a unknown genes are displayed separately on a display.Type: GrantFiled: January 12, 1999Date of Patent: March 20, 2001Assignee: Hitachi, Ltd.Inventors: Hideki Kambara, Chihiro Uematsu