Patents by Inventor Claudia Giessel
Claudia Giessel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11261238Abstract: The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.Type: GrantFiled: February 3, 2020Date of Patent: March 1, 2022Assignee: Hoffmann-La Roche Inc.Inventors: Roberto Falkenstein, Claudia Giessel
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Publication number: 20200377574Abstract: The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.Type: ApplicationFiled: February 3, 2020Publication date: December 3, 2020Inventors: Roberto Falkenstein, Claudia Giessel
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Patent number: 10590186Abstract: The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.Type: GrantFiled: October 19, 2016Date of Patent: March 17, 2020Assignee: HOFFMANN-LA ROCHE INC.Inventors: Roberto Falkenstein, Claudia Giessel
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Publication number: 20170137497Abstract: The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.Type: ApplicationFiled: October 19, 2016Publication date: May 18, 2017Inventors: Roberto Falkenstein, Claudia Giessel
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Patent number: 9493548Abstract: The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.Type: GrantFiled: May 28, 2008Date of Patent: November 15, 2016Assignee: Hoffmann-La Roche Inc.Inventors: Roberto Falkenstein, Claudia Giessel
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Patent number: 9481706Abstract: The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second cType: GrantFiled: April 7, 2014Date of Patent: November 1, 2016Assignee: Hoffmann-La Roche Inc.Inventors: Roberto Falkenstein, Birgit Weydanz, Nicole Fuehrler, Claudia Giessel, Sybille Gabel, Adelbert Grossmann, Friederike Hesse, Marc Pompiati, Andreas Schaubmar, Brigitte Kraemer
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Publication number: 20140220633Abstract: The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second cType: ApplicationFiled: April 7, 2014Publication date: August 7, 2014Applicant: HOFFMANN-LA ROCHE INC.Inventors: Roberto Falkenstein, Birgit Weydanz, Nicole Fuehrler, Claudia Giessel, Sybille Gabel, Adelbert Grossmann, Friederike Hesse, Marc Pompiati, Andreas Schaubmar, Brigitte Kraemer
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Publication number: 20110034672Abstract: The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second cType: ApplicationFiled: January 15, 2009Publication date: February 10, 2011Inventors: Roberto Falkenstein, Birgit Weydanz, Nicole Fuehrler, Claudia Giessel, Sybille Greithanner, Adelbert Grossmann, Friederike Hesse, Marc Pompiati, Andreas Schaubmar, Brigitte Kraemer
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Publication number: 20100311952Abstract: The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.Type: ApplicationFiled: May 28, 2008Publication date: December 9, 2010Inventors: Roberto Falkenstein, Claudia Giessel
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Patent number: 7666629Abstract: The present invention concerns a method for producing recombinant trypsin from porcine pancreas in Pichia pastoris which is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to ?-trypsin and autolysis of ?-trypsin by ?-trypsin into ?-trypsin and from there into inactive peptides.Type: GrantFiled: September 11, 2007Date of Patent: February 23, 2010Assignee: Roche Diagnostics Operations, Inc.Inventors: Rainer Muller, Stephan Glaser, Frank Geipel, Johann-Peter Thalhofer, Bernhard Rexer, Claus Schneider, Michael Ratka, Stephanie Ronning, Hellmut Eckstein, Claudia Giessel
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Publication number: 20080064084Abstract: The present invention concerns a method for producing recombinant trypsin from porcine pancreas in Pichia pastoris which is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to ?-trypsin and autolysis of ?-trypsin by ?-trypsin into ?-trypsin and from there into inactive peptides.Type: ApplicationFiled: September 11, 2007Publication date: March 13, 2008Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: Rainer MULLER, Stephan GLASER, Frank GEIPEL, Johann-Peter THALHOFER, Bernhard REXER, Claus SCHNEIDER, Michael RATKA, Stephanie RONNING, Hellmut ECKSTEIN, Claudia GIESSEL
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Patent number: 7276605Abstract: The present invention concerns a method for producing recombinant trypsin from porcine pancreas in Pichia pastoris which is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to ?-trypsin and autolysis of ?-trypsin by ?-trypsin into ?-trypsin and from there into inactive peptides.Type: GrantFiled: February 1, 2002Date of Patent: October 2, 2007Assignee: Roche Diagnostics Operations, Inc.Inventors: Rainer Muller, Stephan Glaser, Frank Geipel, Johann-Peter Thalhofer, Bernhard Rexer, Claus Schneider, Michael Ratka, Stephanie Ronning, Hellmut Eckstein, Claudia Giessel
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Publication number: 20050142633Abstract: The invention provides a method to produce a protein with carboxypeptidase B activity from a pro-carboxypeptidase B zymogen, derived from a non-animal host organism. Carboxypeptidase B is activated from the zymogen using non-denaturing conditions. Particularly, the activation is performed under conditions that avoid unwanted non-covalent binding of the propeptide to the activated carboxypeptidase B enzyme.Type: ApplicationFiled: December 3, 2004Publication date: June 30, 2005Inventors: Stephan Glaser, Frank Geipel, Thomas Kirschbaum, Bernhard Rexer, Johann-Peter Thalhofer, Rainer Mueller, Claudia Giessel, Hellmut Eckstein, Elvira Wolf
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Publication number: 20040203095Abstract: The present invention concerns a method for producing recombinant trypsin from porcine pancreas in Pichia pastoris which is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to &bgr;-trypsin and autolysis of &bgr;-trypsin by &agr;-trypsin into &egr;-trypsin and from there into inactive peptides.Type: ApplicationFiled: April 12, 2004Publication date: October 14, 2004Inventors: Rainer Muller, Stephan Glaser, Frank Geipel, Johann-Peter Thalhofer, Bernhard Rexer, Claus Schneider, Michael Ratka, Stephanie Ronning, Hellmut Eckstein, Claudia Giessel