Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmPSID2 gene. Some embodiments relate to a promoter or a 5? UTR from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3? UTR or a terminator from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract: A method comprising: obtaining an image; identifying a rotation angle for the image by processing the image with a first neural network; rotating the image by the identified rotation angle to generate a rotated image; classifying the image with a second neural network; and outputting an indication of an outcome of the classification, wherein the first neural network is trained, at least in part, based on a categorical distance between training data and an output that is produced by the first neural network.

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmPSID2 gene. Some embodiments relate to a promoter or a 5’ UTR from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3’ UTR or a terminator from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmPSID2 gene. Some embodiments relate to a promoter or a 5? UTR from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3? UTR or a terminator from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract: A method comprising: obtaining an image; identifying a rotation angle for the image by processing the image with a first neural network; rotating the image by the identified rotation angle to generate a rotated image; classifying the image with a second neural network; and outputting an indication of an outcome of the classification, wherein the first neural network is trained, at least in part, based on a categorical distance between training data and an output that is produced by the first neural network.

Abstract: A well reader and actuator (100) for a well array (50) has an array of detectors, each detector for detecting a fluorescence signal from fluorophores in a respective well of the well array and an excitation subsystem for exciting the fluorophores in the wells of the well array. Embodiments of this invention can be used to carry out two important functions in a highly parallel manner: by addressing individual wells in a 32, 96, 384 etc. well array, where each well contains a potential chemical or biological reaction. The two functions are: 1) through thermal, optical or other means and combinations thereof the rate of a chemical or biological reaction is controlled or gated (e.g. colder wells inhibit a reaction, or an enzymatic reaction requires blue light to proceed); and 2) through use of fluorescent species that are sensitive to the target reaction—or reactions—an optical readout of fluorescent intensity and/or lifetime is tracked to monitor the evolution of the reaction.

Abstract: Provided are compositions and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters, 5?UTR, 3? UTRs, and/or terminators isolated from Glycine max rubisco activase genes.

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmPSID2 gene. Some embodiments relate to a promoter or a 5? UTR from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3? UTR or a terminator from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Abstract: Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using chimeric gene regulatory elements.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using chimeric gene regulatory elements.

Abstract: Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.