Abstract: The present disclosure provides certain RIG-I antagonists. In certain embodiments, the antagonists of the disclosure can be used to treat or prevent a disease or disorder in a subject.

Abstract: A method for estimating a diagnostic score includes adding a first aliquot of a test sample to a first reaction vessel, and a second aliquot of the test sample to a second reaction vessel. The test sample includes a first target nucleic acid and a second target nucleic acid. The second target nucleic acid has a lower expected abundance than the first target nucleic acid. The second aliquot has a larger volume than the first aliquot. A first relative abundance value of the first target nucleic acid and a second relative abundance value of the second target nucleic acid are estimated by performing a first real-time quantitative isothermal amplification assay in the first reaction vessel, and performing a second real-time quantitative isothermal amplification assay in the second reaction vessel, respectively. The diagnostic score is estimated based on the first relative abundance value and the second relative abundance value.

Abstract: The present invention provides a small hairpin nucleic acid molecule that is capable of stimulating interferon production. The nucleic acid molecule of the present invention has a double-stranded section of less than 19 base pairs and at least one blunt end. In certain embodiments, the molecule comprises a 5?-triphosphate or a 5?-diphosphate.

Abstract: The present invention provides a small hairpin nucleic acid molecule that is capable of stimulating interferon production. The nucleic acid molecule of the present invention has a double-stranded section of less than 19 base pairs and at least one blunt end. In certain embodiments, the molecule comprises a 5? triphosphate or a 5? diphosphate.

Abstract: An angle adjustment mechanism for a pump and a motor includes a base, two eccentric bushings and a fixed link. The base has a hinge disposed between a motor flange and a pump flange and a pair of spaced apertures disposed opposite the hinge. The first eccentric bushing has a body portion received in a first of the apertures of the base and the second eccentric bushing has a body portion received in one of a second of the apertures of the base or an inner bore of the first eccentric bushing. The axial center line of the inner bore of the first eccentric bushing is offset from an axial center line of the body portion by a first distance and the axial center line of the inner bore of the second eccentric bushing is offset from an axial center line of the body portion by a second distance, wherein the second distance is different from the first distance.

Abstract: The present invention provides a small hairpin nucleic acid molecule that is capable of stimulating interferon production. The nucleic acid molecule of the present invention has a double-stranded section of less than 19 base pairs and at least one blunt end. In certain embodiments, the molecule comprises a 5? triphosphate or a 5? diphosphate.

Abstract: Disclosed herein are nuclease-based systems for genome editing and methods of using the system for genome editing. Also, disclosed are approaches to enhance Cas9-mediated gene editing efficiency in primary human cells with minimal toxicity when using adeno-associated virus vectors (AAV) to express the guide RNAs necessary for CRISPR/Cas9-based genome editing in the presence of helper proteins.

Abstract: In vivo gene therapies for immune deficiencies are described. The in vivo gene therapies utilize a foamy viral vector including a PGK promoter with a therapeutic gene. The foamy viral vector can be beneficially administered with cell mobilization into the peripheral blood.

Abstract: A computing device executes analytic source code published to it. A revision definition includes source code in a first programming language compiled using a first compiler. When the source code compilation is successful, the source code is parsed to define code elements, an integration function is generated, the integration function is compiled with the source code using a second compiler in a second programming language to define an executable, an entry point address to the generated integration function is retrieved from the defined executable, an incremented revision number and the entry point address are stored in a created revision data structure, and the created revision data structure is stored in a created module context data structure. The integration function maps first input and output parameters of the source code in the second programming language to second input and output parameters of the source code in the first programming language.

Abstract: A pumping apparatus for pumping a liquid from a source to a target including a motor and pump driven by the motor. The pumping apparatus further includes a support frame, a swivel platform pivotably mounted on the support frame and a drive assembly connected with the support frame and the swivel platform for pivoting the swivel platform with respect to the support frame, wherein pivoting of the swivel platform by the drive assembly changes an angle defined between a longitudinal axis of the motor and a longitudinal axis of the pump piston, and wherein a spacer is provided for limiting the range of the angle.

Abstract: Disclosed herein are nuclease-based systems for genome editing and methods of using the system for genome editing. Also, disclosed are approaches to enhance Cas9-mediated gene editing efficiency in primary human cells with minimal toxicity when using adeno-associated virus vectors (AAV) to express the guide RNAs necessary for CRISPR/Cas9-based genome editing in the presence of helper proteins.

Abstract: An angle adjustment mechanism for a pump and a motor includes a base, two eccentric bushings and a fixed link. The base has a hinge disposed between a motor flange and a pump flange and a pair of spaced apertures disposed opposite the hinge. The first eccentric bushing has a body portion received in a first of the apertures of the base and the second eccentric bushing has a body portion received in one of a second of the apertures of the base or an inner bore of the first eccentric bushing. The axial center line of the inner bore of the first eccentric bushing is offset from an axial center line of the body portion by a first distance and the axial center line of the inner bore of the second eccentric bushing is offset from an axial center line of the body portion by a second distance, wherein the second distance is different from the first distance.

Abstract: The specification and drawings present modifications of a footwear (leg wear) and corresponding application methods for using an outer or inner element/compartment attached to the footwear for carrying/holding and draining wet items such as an umbrella. According to an embodiment, a modified footwear may comprise a regular footwear (such as a rain boot) having a foot portion and an upper portion at least partially covering a leg, and an outer/inner element attached at least to the upper portion of the regular footwear and used for holding and draining one or more items (such as folded umbrella) without wetting the leg and inside of the regular footwear (i.e., without introduction of moisture inside of the footwear).

Abstract: Disclosed herein are nuclease-based systems for genome editing and methods of using the system for genome editing. Also, disclosed are approaches to enhance Cas9-mediated gene editing efficiency in primary human cells with minimal toxicity when using adeno-associated virus vectors (AAV) to express the guide RNAs necessary for CRISPR/Cas9-based genome editing in the presence of helper proteins.

Abstract: A pumping apparatus for pumping a liquid from a source to a target including a motor and pump driven by the motor. Sacrificial zinc components are utilized to minimize corrosion due to chlorine vapors. Measures to ensure that liquid is pumped in only one direction include the use of a full wave rectifier and/or a spacer incorporated with a swivel platform to limits the range of the angle between the motor shaft and the pump piston. To minimize the risk of rupturing the pump, the pump piston includes a relieved portion in fluid communication with a transverse bore of the pump housing when the piston is axially inserted within the pump housing in its full forward position. To prevent overheating and damage to the pump components, the pumping apparatus makes use of a positive temperature coefficient (PTC) resistor interposed between the motor and a current source.

Abstract: A foam filled frame member comprises first and second frame components 12, 14 spaced apart from one another and connected to one another by a first bridge 20 and by a second bridge 22, the first and second frame components 12, 14 and first and second bridges 20, 22 together defining an elongate void, a foam material 26 located within the void and substantially filling the void, and a tie member 30 interconnecting the first and second bridges 20, 22 to restrain the first and second bridges 20, 22 against movement towards or away from one another.

Abstract: A method of manufacturing an insulated frame member including first and second frame components held in a spaced relationship by a first, glazing unit facing connector component and by a second connector component, the first and second frame components together defining a void substantially filled with a foamed material, wherein the first connector component is of width greater than or equal to the thickness of the glazing unit.

Abstract: A printing screen comprises a screen printing foil and a sub-frame component secured to an edge of the foil, wherein the foil is formed with cut-outs defining deflectable parts of the foil to be deflected out of the plane of the remainder thereof, and the sub-frame component comprises a fixed lip upon which part of the foil can be received, a recess into which the said deflectable parts of the foil can be deflected, and a moveable lip defining a series of spaced projections, the moveable lip being moveable to a position in which the projections bear against the deflectable parts of the foil, deflecting the deflectable parts into the recess to secure the foil to the sub-frame component.

Abstract: A printing screen comprises a screen printing foil and a sub-frame component secured to an edge of the foil, wherein the foil is formed with cut-outs defining deflectable parts of the foil to be deflected out of the plane of the remainder thereof, and the sub-frame component comprises a fixed lip upon which part of the foil can be received, a recess into which the said deflectable parts of the foil can be deflected, and a moveable lip defining a series of spaced projections, the moveable lip being moveable to a position in which the projections bear against the deflectable parts of the foil, deflecting the deflectable parts into the recess to secure the foil to the sub-frame component.

Abstract: A pumping apparatus for pumping a liquid from a source to a target including a motor and pump driven by the motor. Sacrificial zinc components are utilized to minimize corrosion due to chlorine vapors. Measures to ensure that liquid is pumped in only one direction include the use of a full wave rectifier and/or a spacer incorporated with a swivel platform to limits the range of the angle between the motor shaft and the pump piston. To minimize the risk of rupturing the pump, the pump piston includes a relieved portion in fluid communication with a transverse bore of the pump housing when the piston is axially inserted within the pump housing in its full forward position. To prevent overheating and damage to the pump components, the pumping apparatus makes use of a positive temperature coefficient (PTC) resistor interposed between the motor and a current source.