Patents by Inventor David A. Zarling
David A. Zarling has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Use of consensus sequences for targeted homologous gene isolation and recombination in gene families
Patent number: 6524856Abstract: The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or pathogen screening.Type: GrantFiled: December 11, 1998Date of Patent: February 25, 2003Assignee: Pangene CorporationInventors: Sushma Pati, David A. Zarling, Hong Zeng -
Publication number: 20020147161Abstract: Provided herein are methods for inhibiting cell proliferation in an individual comprising administering to the individual a composition comprising a Rad51 inhibitor. Also provided herein is a method for inhibiting the growth of a cell comprising administering to said cell a composition comprising a Rad51 inhibitor. Such methods can further include the step of providing radiation or DNA damaging agents after administration of said Rad51 inhibitor. Also described herein are methods which are performed in vivo and/or on cancerous cells.Type: ApplicationFiled: December 6, 1999Publication date: October 10, 2002Inventors: HONG ZENG, GURUCHARAN REDDY, ANNE VALLERGA, DAVID A. ZARLING
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Publication number: 20020108136Abstract: The invention relates to methods and compositions for producing transgenic animals by targeted homologous recombination comprising targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence. In certain embodiments, the invention relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention.Type: ApplicationFiled: August 9, 2001Publication date: August 8, 2002Applicant: SRIInventors: Sushma Pati, David Zarling
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Publication number: 20020094555Abstract: The invention relates to methods for inhibiting, cloning, modifying or labelling an endogenous DNA sequence using compositions comprising recombinases in combination with exogenous polynucleotides containing “anchoring” or “locking” sequences. The anchoring sequences serve to stabilize structures formed by the exogenous polynucleotides and the endogenous DNA. The stabilized structure thus can either serve to regulate gene transcription or replication, or can allow the endogenous sequences to be labelled or pulled out, i.e. cloned, or modified.Type: ApplicationFiled: April 21, 2000Publication date: July 18, 2002Inventors: Boris P. Belotserkovskii, Gurucharan Reddy, David A. Zarling
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Publication number: 20020090361Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: ApplicationFiled: August 13, 1997Publication date: July 11, 2002Inventors: DAVID ZARLING, ELISSA SENA
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Publication number: 20020086840Abstract: The present invention is directed to methods and compositions for inhibiting or reducing tumor cell proliferation in an individual in vivo. More specifically, a tumor cell is contacted, in vivo, with a Rad51 inhibitor, and a polynucleotide capable of expressing functional p53 protein. In a further embodiment of the present invention the tumor cell is exposed in vivo to radiation or chemotherapeutic agents (e.g., BCNU, CCNU, and DMZ, GB, cisplatin and the like). The Rad51 inhibitor may be selected from the group consisting of peptides, small molecules and Rad51 antisense molecules. The Rad51 antisense molecule and the p53 polynucleotide may be encoded on an expression vector under the control of one or more promoters, and the expression vector may then be incorporated into a viral genome, preferably an andeno or retro virus, which is then used to introduce the expression vector into the tumor cell.Type: ApplicationFiled: January 26, 2001Publication date: July 4, 2002Inventors: David A. Zarling, Gurucharan Reddy
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Patent number: 6399397Abstract: The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.Type: GrantFiled: October 18, 2000Date of Patent: June 4, 2002Assignee: SRI InternationalInventors: David A. Zarling, Michel J. Rossi, Norman A. Peppers, James Kane, Gregory W. Faris, Mark J. Dyer, Steve Y. Ng, Luke V. Schneider
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Publication number: 20020061530Abstract: the present invention is directed to methods and compositions for using DNA analog probes in increasing the efficiency of DNA targeting by recombinase coated nucleoprotein filaments. The present invention teaches novel methods and compositions that combine the traditional advantages of RecA coated filaments in catalyzing homology searches with the utility of PNA (peptide nucleic acids) to bind DNA with a very high affinity and its ability to locally open the target DNA thereby improving the kinetics of RecA-mediated strand exchange.Type: ApplicationFiled: July 30, 2001Publication date: May 23, 2002Inventors: Boris P. Belotserkovskii, Gurucharan Reddy, David A. Zarling
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Publication number: 20020032530Abstract: The present invention is directed to methods and systems for integrating the provision of genomics services and the production of genomics products. In one aspect of the invention, a method is provided for integrated genomic services comprising (a) receiving a first request from a customer, wherein said request comprises a first nucleic acid sequence, and an order for at least two genomics products or services; and (b) utilizing said nucleic acid sequence to provide said at least two genomics services or products.Type: ApplicationFiled: February 12, 2001Publication date: March 14, 2002Inventors: Sushma Pati, Raya Zion, Ian Alexander Fairwether Black, Christopher Lehman, Ron Caspi, Kathryn M. Stephens, David Zarling
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Publication number: 20010044107Abstract: The present invention is directed to the use of recombinases such as E. coli recA to mediate the detection of target sequences on gene chips.Type: ApplicationFiled: December 28, 2000Publication date: November 22, 2001Inventors: David A. Zarling, Gurucharan Reddy, Sushma Pati
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Patent number: 6312914Abstract: The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.Type: GrantFiled: October 18, 2000Date of Patent: November 6, 2001Assignee: Orasure Technologies, Inc.Inventors: Keith W. Kardos, R. Sam Niedbala, Jarrett Lee Burton, David E. Cooper, David A. Zarling, Michel J. Rossi, Norman A. Peppers, James Kane, Gregory W. Faris, Mark J. Dyer, Steve Y. Ng, Luke V. Schneider
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Patent number: 6255113Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a eukaryotic cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active eukaryotic cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active eukaryotic cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: GrantFiled: February 8, 1995Date of Patent: July 3, 2001Assignee: SRI InternationalInventors: David A. Zarling, Elissa P. Sena
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Patent number: 6200812Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: GrantFiled: April 8, 1999Date of Patent: March 13, 2001Assignee: SRI InternationalInventors: Sushma Pati, David A. Zarling
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Patent number: 6159686Abstract: The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.Type: GrantFiled: January 30, 1998Date of Patent: December 12, 2000Assignee: SRI InternationalInventors: Keith W. Kardos, R. Sam Niedbala, Jarrett Lee Burton, David E. Cooper, David A. Zarling, Michel J. Rossi, Norman A. Peppers, James Kane, Gregory W. Faris, Mark J. Dyer, Steve Y. Ng, Luke V. Schneider
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Patent number: 6074853Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: GrantFiled: August 14, 1998Date of Patent: June 13, 2000Assignee: SRIInventors: Sushma Pati, David A. Zarling
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Patent number: 5948653Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: GrantFiled: August 13, 1997Date of Patent: September 7, 1999Inventors: Sushma Pati, David A. Zarling
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Patent number: 5891656Abstract: The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.Type: GrantFiled: July 2, 1997Date of Patent: April 6, 1999Assignee: SRI InternationalInventors: David A. Zarling, Michel J. Rossi, Norman A. Peppers, James Kane, Gregory W. Faris, Mark J. Dyer, Steve Y. Ng, Luke V. Schneider
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Patent number: 5763240Abstract: The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a eukaryotic cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active eukaryotic cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active eukaryotic cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell.Type: GrantFiled: July 14, 1994Date of Patent: June 9, 1998Assignee: SRI InternationalInventors: David A. Zarling, Elissa P. Sena
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Patent number: 5736410Abstract: The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.Type: GrantFiled: June 7, 1995Date of Patent: April 7, 1998Assignee: SRI InternationalInventors: David A. Zarling, Michel J. Rossi, Norman A. Peppers, James Kane, Gregory W. Faris, Mark J. Dyer, Steve Y. Ng, Luke V. Schneider
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Patent number: 5719023Abstract: A method of identifying the presence of a known target sequence in nucleic acid contained in a fixed cellular or subcellular biological structure. By adding a stable, reporter-labeled RecA/single-stranded probe complex to the cellular or subcellular structure, the target sequence can be effectively labeled by in situ hybridization, allowing the target sequence to be visualized histologically and microscopically or detected by in situ cytometry or cell sorting flow techniques.Type: GrantFiled: June 3, 1994Date of Patent: February 17, 1998Assignee: Daikin Industries, Ltd.Inventors: David A. Zarling, Cornelia J. Calhoun, Elissa P. Sena