Patents by Inventor David D. Nolte

David D. Nolte has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10642014
    Abstract: An apparatus for viewing a biological sample that functions as both a microscope and an interferometer. A short-coherence light source directs light onto the sample. A Fourier transform lens and a pixel-array detector are positioned to collect light scattered by the sample. An optic fiber assembly conveys a reference beam from the short-coherence light source. The detector collects the reference beam and the signal beam and uses coherence gating to acquire interferometric image data. In some embodiments the axis of the incident light striking the sample and the axis of collected scattered signal light form an angle of less than 180 degrees and advantageously an angle between 120 and 150 degrees. A method of converting a microscope into an interferometer is also disclosed.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: May 5, 2020
    Assignee: Purdue Research Foundation
    Inventors: David D. Nolte, John J. Turek, Ran An
  • Publication number: 20190376110
    Abstract: Biodynamic imaging (BDI) performs Doppler spectroscopy of intracellular motion in living samples. The present disclosure describes novel methods and systems to perform: 1) BDI of living 3D tissue culture exposed to bacteria; 2) BDI of living biopsies exposed to bacteria; 3) BDI of infected tissues responding to antibiotics. A novel new element is the use of immortalized cancer cells to generate tissues that act as “biosensors” or “reporters” of bacterial infection, for cells as found directly in aqueous samples and cells that have been concentrated through filtration, centrifugation or a combination while maintaining them in a viable form. Pathogenicity is assessed through the spectral Doppler signatures of the changes in tissue dynamics induced by the bacteria.
    Type: Application
    Filed: June 7, 2019
    Publication date: December 12, 2019
    Applicant: Purdue Research Foundation
    Inventors: Michael R. Ladisch, David D. Nolte, John J. Turek, Eduardo de Aquino Ximenes, Honggu Choi
  • Patent number: 10426348
    Abstract: A phenotypic profiling method for drug/dose physiological response of living bodies utilizes feature recognition to segment the information in time-frequency tissue-response spectrograms to construct N-dimensional feature vectors. The feature vectors are used to generate a correlation matrix among a large number of different stimuli in the form of drugs, doses and conditions. Multi-dimensional scaling is applied to the correlation matrix to form a two-dimensional map of response relationships that retains rank distances from the higher-dimensionality feature matrix. The two-dimensional phenotypic profile space displays compact regions indicative of particular physiological responses, such as regions of enhanced active transport, membrane undulations and blebbing.
    Type: Grant
    Filed: February 6, 2013
    Date of Patent: October 1, 2019
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventors: David D. Nolte, John J. Turek
  • Patent number: 10401793
    Abstract: Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, which should not be confused with mechanical motion of the system. Apparatus for motility contrast imaging that provides increased mechanical stability are disclosed. It is based on common-path configurations, in which the signal and reference beams share optical elements in their paths to the detector. The two beams share mechanical motions in common, and hence those motions do not contribute to the signal.
    Type: Grant
    Filed: November 22, 2016
    Date of Patent: September 3, 2019
    Inventor: David D. Nolte
  • Patent number: 10101147
    Abstract: A system for motility contrast imaging a biological target within tissue comprising a CCD array; an illumination source for generating an incoming beam; a first beam splitter for receiving the incoming beam and producing an object beam and a reference beam; a second beam splitter for illuminating a multitude of biological targets with the object beam and for directing backscattered object beams towards the CCD array; a computer-controlled delay stage for zero-path-matching the reference beam to the backscattered object beams; a reference beam that intersects the backscattered object beams at an angle to produce a series of interference fringes that modulate Fourier-domain information; and a computer for receiving a time series of Fourier-domain information. The interference fringes between the backscattered object beam and the reference beam are recorded by the CCD array and passed to the computer which constructs a digital hologram at successive times.
    Type: Grant
    Filed: November 10, 2014
    Date of Patent: October 16, 2018
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventors: David D. Nolte, John J. Turek, Kwan Jeong
  • Patent number: 9977859
    Abstract: Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, not to be confused with mechanical motion of the system. The use of the resulting spectrogram response signatures, or “fingerprint” data, of known compounds is disclosed to screen new compounds for leads as to those having potentially beneficial mechanisms of action. The “fingerprint” data of known toxic compounds can be used to screen new compounds for toxicity.
    Type: Grant
    Filed: June 17, 2011
    Date of Patent: May 22, 2018
    Assignee: PURDUE RESERACH FOUNDATION
    Inventors: David D. Nolte, Kwan Jeong
  • Patent number: 9829482
    Abstract: A method and system is provided for evaluating viability of oocytes and embryos comprising imaging an oocyte or embryo using motility contrast imaging; generating temporal contrast and spatial contrast data for the cells; generating a cell viability value as a function of the temporal and spatial contrast data; and comparing the cell viability value to a predetermined value indicative of a cell suitable for use in an in vitro fertilization program.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: November 28, 2017
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventors: Ran An, David D. Nolte, Zoltan Machaty, John J. Turek
  • Patent number: 9723989
    Abstract: In a method for ex vivo evaluation of tissue response, a target biological sample is placed in a chamber of a sample holder. Biodynamic imaging (BDI) is performed on the sample to extract BDI data of the entire sample, optical coherence imaging (OCI) data is generated from the BDI data; and then motility contrast imaging (MCI) data is generated from the OCI data. The MCI data is used to select an area of the ex vivo sample having the highest normalized standard deviation (NSD) value, indicative of a region of desirable responsiveness to a stimuli. The sample is subjected to a perturbation or external condition and an MCI analysis is performed on the selected area to determine the tissue response to the perturbation or external condition. In one aspect, the selected area or region of interest is obtained using a gradient descent method.
    Type: Grant
    Filed: January 19, 2016
    Date of Patent: August 8, 2017
    Inventors: David D. Nolte, John J. Turek, Ran An
  • Publication number: 20170156598
    Abstract: A phenotypic profiling method for drug/dose physiological response of living bodies utilizes feature recognition to segment the information in time-frequency tissue-response spectrograms to construct N-dimensional feature vectors. The feature vectors are used to generate a correlation matrix among a large number of different stimuli in the form of drugs, doses and conditions. Multi-dimensional scaling is applied to the correlation matrix to form a two-dimensional map of response relationships that retains rank distances from the higher-dimensionality feature matrix. The two-dimensional phenotypic profile space displays compact regions indicative of particular physiological responses, such as regions of enhanced active transport, membrane undulations and blebbing.
    Type: Application
    Filed: February 6, 2013
    Publication date: June 8, 2017
    Applicant: PURDUE RESEARCH FOUNDATION
    Inventors: David D. Nolte, John J. Turek
  • Publication number: 20170131682
    Abstract: Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, which should not be confused with mechanical motion of the system. Apparatus for motility contrast imaging that provides increased mechanical stability are disclosed. It is based on common-path configurations, in which the signal and reference beams share optical elements in their paths to the detector. The two beams share mechanical motions in common, and hence those motions do not contribute to the signal.
    Type: Application
    Filed: November 22, 2016
    Publication date: May 11, 2017
    Inventor: David D. Nolte
  • Publication number: 20170102376
    Abstract: A method and system is provided for evaluating viability of oocytes and embryos comprising imaging an oocyte or embryo using motility contrast imaging; generating temporal contrast and spatial contrast data for the cells; generating a cell viability value as a function of the temporal and spatial contrast data; and comparing the cell viability value to a predetermined value indicative of a cell suitable for use in an in vitro fertilization program.
    Type: Application
    Filed: September 13, 2013
    Publication date: April 13, 2017
    Applicant: Purdue Research Foundation
    Inventors: Ran An, David D. Nolte, Zoltan Machaty, John J. Turek
  • Patent number: 9514271
    Abstract: Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, which should not be confused with mechanical motion of the system. Apparatus for motility contrast imaging that provides increased mechanical stability is disclosed. It is based on common-path configurations, in which the signal and reference beams share optical elements in their paths to the detector. The two beams share mechanical motions in common, and hence these motions do not contribute to the signal.
    Type: Grant
    Filed: June 17, 2011
    Date of Patent: December 6, 2016
    Assignee: Purdue Research Foundation
    Inventor: David D. Nolte
  • Publication number: 20160266365
    Abstract: An apparatus for viewing a biological sample that functions as both a microscope and an interferometer. A short-coherence light source directs light onto the sample. A Fourier transform lens and a pixel-array detector are positioned to collect light scattered by the sample. An optic fiber assembly conveys a reference beam from the short-coherence light source. The detector collects the reference beam and the signal beam and uses coherence gating to acquire interferometric image data. In some embodiments the axis of the incident light striking the sample and the axis of collected scattered signal light form an angle of less than 180 degrees and advantageously an angle between 120 and 150 degrees. A method of converting a microscope into an interferometer is also disclosed.
    Type: Application
    Filed: March 11, 2016
    Publication date: September 15, 2016
    Inventors: David D. Nolte, John J. Turek, Ran An
  • Publication number: 20160210748
    Abstract: In a method for ex vivo evaluation of tissue response, a target biological sample is placed in a chamber of a sample holder. Biodynamic imaging (BDI) is performed on the sample to extract BDI data of the entire sample, optical coherence imaging (OCI) data is generated from the BDI data; and then motility contrast imaging (MCI) data is generated from the OCI data. The MCI data is used to select an area of the ex vivo sample having the highest normalized standard deviation (NSD) value, indicative of a region of desirable responsiveness to a stimuli. The sample is subjected to a perturbation or external condition and an MCI analysis is performed on the selected area to determine the tissue response to the perturbation or external condition. In one aspect, the selected area or region of interest is obtained using a gradient descent method.
    Type: Application
    Filed: January 19, 2016
    Publication date: July 21, 2016
    Inventors: David D. Nolte, John J. Turek, Ran An
  • Publication number: 20150127309
    Abstract: A method is provided to translate from tissue dynamics spectroscopy (TDS) data formats into high-content analysis (HCA) data formats. The method utilizes TDS feature vectors and HCA feature vectors obtained from a shared set of compounds and cell lines to generate a translation matrix. The translator is applied to the unique data format of TDS that carries information from deep inside 3D tissue to convert the data into a standard data 2D HCA data format that fits into the standard workflow of potential customers.
    Type: Application
    Filed: October 29, 2014
    Publication date: May 7, 2015
    Inventors: Ran Am, David D. Nolte, John J. Turek
  • Publication number: 20150124259
    Abstract: Systems and methods for imaging small (˜1 mm thick) living biological specimen is provided to enable the generation of functional 3D images of living tissue for evaluating the effect of an external perturbation on the health of the specimen. A fluctuation power spectrum is constructed for each pixel of a holographic 3D image of the specimen over time and subject to the external perturbation. A normalized spectrum of dynamic intensity as a function of frequency is generated for each pixel. The normalized spectra for each pixel is filtered according to a selected frequency range from among characteristic frequencies corresponding to dynamic activity of naturally occurring biological events within the specimen to provide data corresponding only to the dynamic activity associated with the selected frequency range.
    Type: Application
    Filed: October 28, 2014
    Publication date: May 7, 2015
    Inventors: Ran An, David D. Nolte, John J. Turek
  • Publication number: 20150079621
    Abstract: A method and system is provided for evaluating viability of oocytes and embryos comprising imaging an oocyte or embryo using motility contrast imaging; generating temporal contrast and spatial contrast data for the cells; generating a cell viability value as a function of the temporal and spatial contrast data; and comparing the cell viability value to a predetermined value indicative of a cell suitable for use in an in vitro fertilization program.
    Type: Application
    Filed: September 13, 2013
    Publication date: March 19, 2015
    Applicant: Purdue Research Foundation
    Inventors: Ran An, David D. Nolte, Zoltan Machaty, John J. Turek
  • Publication number: 20150062592
    Abstract: A system for motility contrast imaging a biological target within tissue comprising a CCD array; an illumination source for generating an incoming beam; a first beam splitter for receiving the incoming beam and producing an object beam and a reference beam; a second beam splitter for illuminating a multitude of biological targets with the object beam and for directing backscattered object beams towards the CCD array; a computer-controlled delay stage for zero-path-matching the reference beam to the backscattered object beams; a reference beam that intersects the backscattered object beams at an angle to produce a series of interference fringes that modulate Fourier-domain information; and a computer for receiving a time series of Fourier-domain information. The interference fringes between the backscattered object beam and the reference beam are recorded by the CCD array and passed to the computer which constructs a digital hologram at successive times.
    Type: Application
    Filed: November 10, 2014
    Publication date: March 5, 2015
    Inventors: David D. Nolte, John J. Turek, Kwan Jeong
  • Patent number: 8886295
    Abstract: A system for motility contrast imaging a biological target within tissue comprising a CCD array; an illumination source for generating an incoming beam; a first beam splitter for receiving the incoming beam and producing an object beam and a reference beam; a second beam splitter for illuminating a multitude of biological targets with the object beam and for directing backscattered object beams towards the CCD array; a computer-controlled delay stage for zero-path-matching the reference beam to the backscattered object beams; a reference beam that intersects the backscattered object beams at an angle to produce a series of interference fringes that modulate Fourier-domain information; and a computer for receiving a time series of Fourier-domain information. The interference fringes between the backscattered object beam and the reference beam are recorded by the CCD array and passed to the computer which constructs a digital hologram at successive times.
    Type: Grant
    Filed: September 2, 2010
    Date of Patent: November 11, 2014
    Assignee: Purdue Research Foundation
    Inventors: David D. Nolte, John J. Turek, Kwan Jeong
  • Publication number: 20130096017
    Abstract: Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, not to be confused with mechanical motion of the system. The use of the resulting spectrogram response signatures, or “fingerprint” data, of known compounds is disclosed to screen new compounds for leads as to those having potentially beneficial mechanisms of action. The “fingerprint” data of known toxic compounds can be used to screen new compounds for toxicity.
    Type: Application
    Filed: June 17, 2011
    Publication date: April 18, 2013
    Applicant: PURDUE RESEARCH FOUNDATION
    Inventors: David D. Nolte, Kwan Jeong