Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

Abstract: A vertical clamping device is provided that supports a flow cell component in a vertical configuration in which the flow cell is on an opposite side of the vertical support from the electronic interface. The clamp includes a vertical setting to receive the flow cell component and provides an electronic interface on a vertical surface of the vertical setting. A block supports the fluidics interface and can move in a horizontal direction bringing the fluidics interface into contact with the flow cell component.

Abstract: A vertical clamping device is provided that supports a flow cell component in a vertical configuration in which the flow cell is on an opposite side of the vertical support from the electronic interface. The clamp includes a vertical setting to receive the flow cell component and provides an electronic interface on a vertical surface of the vertical setting. A block supports the fluidics interface and can move in a horizontal direction bringing the fluidics interface into contact with the flow cell component.

Abstract: Systems and methods identify a product ion that does not include an interference. A full product ion spectrum for a mass range of an analyte in a sample is received from a tandem mass spectrometer. A first set of one or more peak parameters is calculated for a product ion in the full product ion spectrum using a first XIC window width. A second set of one or more peak parameters is calculated for the product ion using a second XIC window width. The product ion is identified as not including an interference, if the first set of one or more peak parameters and the second set of one or more peak parameters are substantially the same. The product ion is further confirmed or determined to be from the analyte and not from a matrix of the sample by correlating the product to a precursor ion of the analyte.

Abstract: A vertical clamping device is provided that supports a flow cell component in a vertical configuration in which the flow cell is on an opposite side of the vertical support from the electronic interface. The clamp includes a vertical setting to receive the flow cell component and provides an electronic interface on a vertical surface of the vertical setting. A block supports the fluidics interface and can move in a horizontal direction bringing the fluidics interface into contact with the flow cell component.

Abstract: Methods and systems are provided for triggering an information dependent mass spectrometry scan in real time. A mass spectrometry scan of a separating sample mixture is performed by a mass spectrometer at each time interval of a time period. The mass spectrometer receives the separating sample mixture from a separation device. It is determined at a certain time interval that a received mass spectrometry scan at the time interval and one or more preceding received mass spectrometry scans include two or more time-varying ion signals that represent two or more fragment ion transitions of a known compound. If a characteristic of the two or more time-varying ion signals meets a selection criterion, the mass spectrometer is instructed to perform a dependent mass spectrometry scan of the separating sample mixture for a precursor ion of the known compound at the time interval.

Abstract: A microfluidic manipulation system is provided that includes a blade for manipulating deformable material and at least one movable support that is capable of moving the blade into contact with a microfluidic device including a deformable feature. When the microfluidic device is operatively held by a holder, a movable support can position the distal end of the blade relative to the microfluidic device and move the contact tip surface of the blade such that it deforms the deformable feature.

Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Abstract: A system and method are provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions. A sample is retained in a pouch or flexible bag which permits bulk PCR amplification with efficient heat-transfer properties. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.

Abstract: A device, system, and method are provided for thermally treating a fluid processing device. According to various embodiments, a system is provided that can include a thermal device and a fluid processing device holder. The thermal device can include a first block having a thermal conductivity greater than 0.5 Watt per centimeter Kelvin (W/cm·K), a second block having a thermal conductivity greater than 0.5 W/cm·K, and a heat-pump device disposed between the first block and the second block. The heat-pump device can transfer thermal energy from at least one of the first block and the second block to the other of the first block and the second block. The fluid processing device holder can hold a fluid processing device in a heat-transfer position with respect to the first block and the second block. The fluid processing device can be a microfluidic device.

Abstract: A microfluidic device is provided that can include an input liquid-containment feature. The microfluidic device can include an overflow channel in fluid communication with the input liquid-containment feature. The microfluidic device can include a fluid capture appendix in fluid communication with the overflow channel.

Abstract: A device is provided that can include at least one gas trap that can be arranged in fluid communication with a sample-containment feature formed in or on the device. The gas trap can be arranged to trap gas or air displaced from the sample-containment feature as the sample-containment feature is loaded with a liquid. The trapped gas in the gas trap can assist in breaking-up and expelling the liquid from the sample-containment feature during a subsequent liquid transfer operation, for example, to an adjacent sample-containment feature. Systems for processing such a device and methods using such a device are also provided.

Abstract: A device, system, and method are provided for thermally treating a fluid processing device. According to various embodiments, a system is provided that can include a thermal device and a fluid processing device holder. The thermal device can include a first block having a thermal conductivity greater than 0.5 Watt per centimeter Kelvin (W/cm·K), a second block having a thermal conductivity greater than 0.5 W/cm·K, and a heat-pump device disposed between the first block and the second block. The heat-pump device can transfer thermal energy from at least one of the first block and the second block to the other of the first block and the second block. The fluid processing device holder can hold a fluid processing device in a heat-transfer position with respect to the first block and the second block. The fluid processing device can be a microfluidic device.

Abstract: A fluid processing device is provided that comprises a substrate including a surface and a fluid processing pathway at least partially formed in or on the surface. The fluid processing pathway can comprise a channel, a reaction region in fluid communication with the channel, a microarray in fluid communication with the channel, and optionally a deformable valve. The microarray can comprise binding and/or detection sites, and each site can comprise a binding moiety. A method and a system using the fluid processing device, are also provided.

Abstract: A device, system, and method are provided for thermally treating a fluid processing device. According to various embodiments, a system is provided that can include a thermal device and a fluid processing device holder. The thermal device can include a first block having a thermal conductivity greater than 0.5 Watt per centimeter Kelvin (W/cm·K), a second block having a thermal conductivity greater than 0.5 W/cm·K, and a heat-pump device disposed between the first block and the second block. The heat-pump device can transfer thermal energy from at least one of the first block and the second block to the other of the first block and the second block. The fluid processing device holder can hold a fluid processing device in a heat-transfer position with respect to the first block and the second block. The fluid processing device can be a microfluidic device.

Abstract: A device is provided that can include at least one gas trap that can be arranged in fluid communication with a sample-containment feature formed in or on the device. The gas trap can be arranged to trap gas or air displaced from the sample-containment feature as the sample-containment feature is loaded with a liquid. The trapped gas in the gas trap can assist in breaking-up and expelling the liquid from the sample-containment feature during a subsequent liquid transfer operation, for example, to an adjacent sample-containment feature. Systems for processing such a device and methods using such a device are also provided.

Abstract: A microfluidic device is provided that can include an input liquid-containment feature. The microfluidic device can include an overflow channel in fluid communication with the input liquid-containment feature. The microfluidic device can include a fluid capture appendix in fluid communication with the overflow channel.

Abstract: A microfluidic device is provided that can include at least one trap for receiving displaceable adhesion material displaced during a closing operation of a deformable valve. The trap can be formed as a recess or chamber in a substrate of the microfluidic device. A system including a deformer for opening such a valve is also provided as are methods of opening such a valve.

Abstract: A device is provided that can include at least one gas trap that can be arranged in fluid communication with a sample-containment feature formed in or on the device. The gas trap can be arranged to trap gas or air displaced from the sample-containment feature as the sample-containment feature is loaded with a liquid. The trapped gas in the gas trap can assist in breaking-up and expelling the liquid from the sample-containment feature during a subsequent liquid transfer operation, for example, to an adjacent sample-containment feature. Systems for processing such a device and methods using such a device are also provided.