Patents by Inventor Dino Zanette

Dino Zanette has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6573366
    Abstract: A process for purifying human Interleukin-1 receptor antagonist (IL-1ra), obtained by fermenting a strain of recombinant E. coli, which comprises: a) loading the mixture to be purified, which has been buffered at a pH value lower than 6.2 and optionally diluted to reduce its ionic strength at a low value, onto a cationic exchange matrix and eluting said matrix with an aqueous buffered solution having a pH value from about 7.5 to 9.0 and a low ionic strength; and b) applying the eluate from the cationic exchange step, containing the desired IL-1ra protein, directly onto an anionic exchange matrix and eluting with an aqueous buffered solution having a pH value within the above range 7.5 to 9.0 and an increased ionic strength.
    Type: Grant
    Filed: May 9, 2000
    Date of Patent: June 3, 2003
    Assignee: Gruppo Lepetit S.p.A.
    Inventors: Dino Zanette, Edoardo Giacomo Sarubbi, Adolfo Soffientini
  • Patent number: 5981716
    Abstract: The present invention is directed to a simple and efficient process for the recovery of a biologically active glycoprotein from a biological fluid containing it.In a preferred embodiment, it comprises:a) contacting a filtered culture fluid with a dihydroxyboronyl bearing chromatographic supportb) eluting with a first eluting buffer and contacting this eluate directly with an anion exchange matrix bearing quaternary ammonium functional groupsc) eluting with a second eluting buffer and collecting the product therefrom.
    Type: Grant
    Filed: July 22, 1997
    Date of Patent: November 9, 1999
    Assignee: Gruppo Lepettit, S.P.A.
    Inventors: Dino Zanette, Edoardo Giacomo Sarubbi, Adolfo Soffientini, Ermenegildo Restelli, Armando Grigoletto