Abstract: A nanoscale molecularly imprinted polymer (MIP) thin film for small molecule detection, a method of manufacturing the same, and an electrochemical sensor using the MIP and quantum electrochemical impedance spectroscopy (EIS) are described. A plurality of specific recognition spaces for small molecules of 1000 Da or less are formed in the MIP, a receptor polymer is present at one end of the specific recognition spaces, a redox probe is present in a wire form, and the small molecule is a steroid hormone or a protein. The sensor is useful in point-of-care applications because it exhibits a rapid and reversible small molecule detection ability through a simple electrochemical regeneration process without cumbersome washing and solution replacement steps in the manufacturing process, thereby enabling continuous detection.