Patents by Inventor Donald J. Merlo

Donald J. Merlo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10450549
    Abstract: The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: October 22, 2019
    Assignee: Dow AgroSciences LLC
    Inventors: Carla N. Yerkes, Barbara A. Zilinskas, Donald J. Merlo, Terry R. Wright, Justin Lira, Nicole Arnold, Gary D. Thompson, Ning Zhou, Andrew Worden
  • Publication number: 20180346926
    Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
    Type: Application
    Filed: July 30, 2018
    Publication date: December 6, 2018
    Inventors: Ignacio Mario Larrinua, Donald J. Merlo, Avutu S. Reddy, Arvind Kumar ThirumalaiswamySekhar, Aaron T. Woosley
  • Publication number: 20170183673
    Abstract: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains.
    Type: Application
    Filed: March 8, 2017
    Publication date: June 29, 2017
    Inventors: Donald J. Merlo, Sean M. Russell, Diane Retallack, Aaron Woosley, Tom Meade, Kenneth E. Narva
  • Patent number: 9617551
    Abstract: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains.
    Type: Grant
    Filed: July 29, 2011
    Date of Patent: April 11, 2017
    Assignee: Dow AgroSciences LLC
    Inventors: Donald J. Merlo, Sean M. Russell, Diane M. Retallack, Aaron T. Woosley, Thomas Meade, Kenneth Narva
  • Publication number: 20170088855
    Abstract: The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.
    Type: Application
    Filed: October 6, 2016
    Publication date: March 30, 2017
    Inventors: Carla N. Yerkes, Barbara A. Zilinskas, Donald J. Merlo, Terry R. Wright, Justin Lira, Nicole Arnold, Gary D. Thompson, Ning Zhou, Andrew Worden
  • Patent number: 9434773
    Abstract: This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: September 6, 2016
    Assignee: Dow AgroSciences LLC
    Inventors: Avutu S. Reddy, Sun Yuejin, Donald J. Merlo, Weiting W. Ni, Dayakar Pareddy, Zhifang Gao, Gregory A. Bradfisch
  • Patent number: 9427003
    Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
    Type: Grant
    Filed: April 16, 2012
    Date of Patent: August 30, 2016
    Assignee: Dow AgroSciences LLC
    Inventors: Ignacio M. Larrinua, Donald J. Merlo, Avutu S. Reddy, Arvind Kumar ThirumalaiswamySekhar, Aaron T. Woosley
  • Publication number: 20150267213
    Abstract: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains.
    Type: Application
    Filed: July 29, 2011
    Publication date: September 24, 2015
    Applicant: DOW AGROSCIENCES LLC
    Inventors: Donald J. Merlo, Sean M. Russell, Diane M. Retallack, Aaron T. Woosley, Thomas Meade, Kenneth Narva
  • Patent number: 9127289
    Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No ?-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action.
    Type: Grant
    Filed: November 22, 2010
    Date of Patent: September 8, 2015
    Assignee: Dow AgroSciences LLC
    Inventors: Terry R. Wright, Justin M. Lira, Donald J. Merlo, Nicole L. Arnold
  • Publication number: 20150175672
    Abstract: This disclosure concerns methods for the design of synthetic nucleic acid sequences that encode polypeptide amino acid repeat regions. This disclosure also concerns the use of such sequences to express a polypeptide of interest that comprises amino acid repeat regions, and organisms comprising such sequences.
    Type: Application
    Filed: July 16, 2013
    Publication date: June 25, 2015
    Inventors: Donald J. Merlo, Ignacio Larrinua, Scott Bevan
  • Publication number: 20130067617
    Abstract: This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.
    Type: Application
    Filed: September 14, 2012
    Publication date: March 14, 2013
    Inventors: Avutu S. Reddy, Sun Yuejin, Donald J. Merlo, Weiting W. Ni, Dayakar Pareddy, Zhifang Gao, Gregory A. Bradfisch
  • Publication number: 20120266335
    Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
    Type: Application
    Filed: April 16, 2012
    Publication date: October 18, 2012
    Applicant: DOW AGROSCIENCES LLC
    Inventors: IGNACIO M. LARRINUA, DONALD J. MERLO, AVUTU S. REDDY, ARVIND KUMAR THIRUMALAISWAMYSEKHAR, AARON T. WOOSLEY
  • Patent number: 8283522
    Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
    Type: Grant
    Filed: October 27, 2006
    Date of Patent: October 9, 2012
    Assignee: Dow AgroSciences, LLC.
    Inventors: Terry R. Wright, Justin M. Lira, Terence Anthony Walsh, Donald J. Merlo, Jayakumar P. Samuel, Gaofeng Lin
  • Patent number: 8278505
    Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to a pyridyloxyacetate herbicide. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-13 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
    Type: Grant
    Filed: May 9, 2008
    Date of Patent: October 2, 2012
    Assignee: Dow AgroSciences, LLC.
    Inventors: Justin M. Lira, Erika Megan Snodderley, Andrew E. Robinson, Terry R. Wright, Donald J. Merlo
  • Patent number: 8084418
    Abstract: The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin.
    Type: Grant
    Filed: February 16, 2009
    Date of Patent: December 27, 2011
    Assignee: Dow AgroSciences LLC
    Inventors: Timothy D. Hey, Amanda D. Schleper, Scott A. Bevan, Scott B. Bintrim, Jon C. Michell, Ze Sheng Li, Weiting Ni, Baolong Zhu, Donald J. Merlo, Patricia C. Apel-Birkhold, Thomas Meade
  • Patent number: 8003777
    Abstract: The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5? and 3? untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.
    Type: Grant
    Filed: February 5, 2008
    Date of Patent: August 23, 2011
    Assignee: Dow AgroSciences LLC
    Inventors: Donald J. Merlo, Dong Liu, Stephanie L. Burton, Todd P. Glancy
  • Publication number: 20110203017
    Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
    Type: Application
    Filed: October 27, 2006
    Publication date: August 18, 2011
    Applicant: DOW AGROSCIENCES, LLC
    Inventors: Terry R. Wright, Justin M. Lira, Terence Anthony Walsh, Donald J. Merlo, Jayakumar P. Samuel, Gaofeng Lin
  • Patent number: 7982006
    Abstract: This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cp) ?-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.
    Type: Grant
    Filed: June 23, 2010
    Date of Patent: July 19, 2011
    Assignee: Dow AgroSciences LLC
    Inventors: William M. Ainley, Janna Armstrong, Krishna Madduri, Donald J. Merlo, Kelley A. Smith, Mark A. Thompson, Steven R. Webb, Liu Y. Shen
  • Publication number: 20110124503
    Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No ?-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action.
    Type: Application
    Filed: November 22, 2010
    Publication date: May 26, 2011
    Applicant: Dow AgroSciences LLC
    Inventors: Terry R. Wright, Justin M. Lira, Donald J. Merlo, Nicole L. Arnold
  • Publication number: 20110107455
    Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.
    Type: Application
    Filed: December 7, 2007
    Publication date: May 5, 2011
    Applicant: Dow AgroSciences LLC
    Inventors: Justin M. Lira, Terry R. Wright, Sean M. Russell, Donald J. Merlo, Steven Robert Webb, Nicole L. Arnold, Andrew E. Robinson, Kelley A. Smith