Patents by Inventor Doo-Byoung Oh

Doo-Byoung Oh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8685671
    Abstract: The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ?-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: April 1, 2014
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Jeong Seok Park, Moo Woong Kim, Eun Jung Kim, Hye Yun Moon, Doo Byoung Oh, Joo Hyung Heo, Sang Ki Rhee
  • Patent number: 8323926
    Abstract: The present invention relates to a novel H. polymorpha gene regulating Unfolded Protein Response (UPR) and a method for improving the efficiency of secretory expression of a recombinant protein using the same, more particularly, a method for improving the efficiency of secretory expression of a recombinant protein by identifying, modifying, and optimizing the HpHAC1 gene, which encodes a key transcription regulatory factor of the UPR mechanism in H. polymorpha, and a regulatory mechanism mediated by the gene. According to the present invention, the secretory expression system of H. polymorpha can be used for the mass-production of secretory proteins for industrial and medical applications, thereby producing a large amount of the proteins at low cost. Accordingly, the method is employed in the production of the proteins for industrial and medical use, so as to reduce economic burden for a patient, and contribute to the improvement in the welfare of all mankind.
    Type: Grant
    Filed: June 13, 2007
    Date of Patent: December 4, 2012
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Hye-Yun Moon, Doo-Byoung Oh, Sang Ki Rhee
  • Publication number: 20120202248
    Abstract: The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ?-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
    Type: Application
    Filed: April 19, 2012
    Publication date: August 9, 2012
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Jeong Seok Park, Moo Woong Kim, Eun Jung Kim, Hye Yun Moon, Doo Byoung Oh, Joo Hyung Heo, Sang Ki Rhee
  • Patent number: 8236520
    Abstract: The present invention relates to beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
    Type: Grant
    Filed: November 22, 2010
    Date of Patent: August 7, 2012
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Jae Kap Jeong, Ohsuk Kwon, Doo-Byoung Oh, Seonghun Kim
  • Patent number: 8187858
    Abstract: The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
    Type: Grant
    Filed: October 26, 2006
    Date of Patent: May 29, 2012
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Jeong Seok Park, Moo Woong Kim, Eun Jung Kim, Hye Yun Moon, Doo Byoung Oh, Joo Hyung Heo, Sang Ki Rhee
  • Publication number: 20110287463
    Abstract: The present invention relates to a novel beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
    Type: Application
    Filed: November 22, 2010
    Publication date: November 24, 2011
    Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Hyun Ah Kang, Jae Kap Jeong, Ohsuk Kwon, Doo-Byoung Oh, Seonghun Kim
  • Patent number: 8003349
    Abstract: The present invention relates to a novel YlMPO1 gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPO1 gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.
    Type: Grant
    Filed: November 30, 2007
    Date of Patent: August 23, 2011
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Jeong-Nam Park, Yunkyoung Song, Jeong-Yoon Kim, Doo-Byoung Oh, Hyun Ah Kang
  • Publication number: 20100297694
    Abstract: The present invention relates to a novel H. polymorpha gene regulating Unfolded Protein Response (UPR) and a method for improving the efficiency of secretory expression of a recombinant protein using the same, more particularly, a method for improving the efficiency of secretory expression of a recombinant protein by identifying, modifying, and optimizing the HpHAC1 gene, which encodes a key transcription regulatory factor of the UPR mechanism in H. polymorpha, and a regulatory mechanism mediated by the gene. According to the present invention, the secretory expression system of H. polymorpha can be used for the mass-production of secretory proteins for industrial and medical applications, thereby producing a large amount of the proteins at low cost. Accordingly, the method is employed in the production of the proteins for industrial and medical use, so as to reduce economic burden for a patient, and contribute to the improvement in the welfare of all mankind.
    Type: Application
    Filed: June 13, 2007
    Publication date: November 25, 2010
    Applicant: Korea research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Hye-Yun Moon, Doo-Byoung Oh, Sang Ki Rhee
  • Publication number: 20100227362
    Abstract: The present invention relates to a novel YlMPOl gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPOl gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.
    Type: Application
    Filed: November 30, 2007
    Publication date: September 9, 2010
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Jeong-Nam Park, Yunkyoung Song, Jeong-Yoon Kim, Doo-Byoung Oh, Hyun Ah Kang
  • Publication number: 20100062485
    Abstract: The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
    Type: Application
    Filed: October 26, 2006
    Publication date: March 11, 2010
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Jeong Seok Park, Moo Woong Kim, Eun Jung Kim, Hye Yun Moon, Doo Byoung Oh, Joo Hyung Heo, Sang Ki Rhee
  • Publication number: 20080248019
    Abstract: The present invention relates to a novel beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
    Type: Application
    Filed: June 25, 2007
    Publication date: October 9, 2008
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Jae Kap Jeong, Ohsuk Kwon, Doo-Byoung Oh, Seonghun Kim