Patents by Inventor Elena V. Bolchakova
Elena V. Bolchakova has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170175093Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.Type: ApplicationFiled: March 6, 2017Publication date: June 22, 2017Inventors: Elena V. BOLCHAKOVA, James ROZZELLE
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Publication number: 20160312195Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.Type: ApplicationFiled: July 13, 2016Publication date: October 27, 2016Inventors: James E. ROZZELLE, Elena V. Bolchakova
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Patent number: 9382522Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus scotoductus. The invention also provides methods for using these nucleic acids and polypeptides.Type: GrantFiled: August 19, 2009Date of Patent: July 5, 2016Assignee: APPLIED BIOSYSTEMS, LLCInventors: Elena V. Bolchakova, James E. Rozzelle
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Patent number: 8318469Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.Type: GrantFiled: December 13, 2005Date of Patent: November 27, 2012Assignee: Applied Biosystems, LLCInventors: James E. Rozzelle, Elena V. Bolchakova
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Publication number: 20120082979Abstract: Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating misligation products using a particular ligase under reaction conditions and using reagents where that ligase is at least less prone to misligation. In certain embodiments, the recombinant ligase from Archaeoglobus fulgidus (Afu) is employed due to its unique misligation properties.Type: ApplicationFiled: July 18, 2011Publication date: April 5, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Achim E. KARGER, James E. Rozzelle, Chien-Wei Chang, Elena V. Bolchakova
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Publication number: 20100323406Abstract: The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.Type: ApplicationFiled: August 17, 2009Publication date: December 23, 2010Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware CorporationInventors: Paolo Vatta, John W. Brandis, Elena V. Bolchakova, Sandra L. Spurgeon
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Publication number: 20100086970Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus scotoductus. The invention also provides methods for using these nucleic acids and polypeptides.Type: ApplicationFiled: August 19, 2009Publication date: April 8, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Elena V. BOLCHAKOVA, James E. Rozzelle
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Publication number: 20090311709Abstract: Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating misligation products using a particular ligase under reaction conditions and using reagents where that ligase is at least less prone to misligation. In certain embodiments, the recombinant ligase from Archaeoglobus fulgidus (Afu) is employed due to its unique misligation properties.Type: ApplicationFiled: July 14, 2009Publication date: December 17, 2009Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Achim E. KARGER, James E. Rozzelle, Chien-Wei Chang, Elena V. Bolchakova
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Publication number: 20090209008Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.Type: ApplicationFiled: August 18, 2008Publication date: August 20, 2009Applicant: APPLIED BIOSYSTEMS INC.Inventors: JAMES E. ROZZELLE, ELENA V. BOLCHAKOVA
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Publication number: 20090068665Abstract: Ligation-based methods and kits are disclosed for identifying at least two target nucleotides in a mixed population sample that is a sample that contains or potentially contains target nucleic acid sequences from more than one source. Typically, two ligation reaction compositions are formed ligation products generated, and the ligation products or their surrogates are analyzed to identify target nucleotides in the mixed population sample. In certain embodiments, the target nucleic acid sequences, the ligation products, or both are amplified. In certain embodiments, multiplex amplification and/or ligation reactions are performed.Type: ApplicationFiled: July 31, 2008Publication date: March 12, 2009Applicant: APPLIED BIOSYSTEMS INC.Inventors: Achim E. KARGER, Elena V. BOLCHAKOVA
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Patent number: 7427479Abstract: Ligation-based methods and kits are disclosed for identifying at least two target nucleotides in a mixed population sample, that is a sample that contains or potentially contains target nucleic acid sequences from more than one source. Typically, two ligation reaction compositions are formed, ligation products generated, and the ligation products or their surrogates are analyzed to identify target nucleotides in the mixed population sample. In certain embodiments, the target nucleic acid sequences, the ligation products, or both are amplified. In certain embodiments, multiplex amplification and/or ligation reactions are performed.Type: GrantFiled: April 29, 2005Date of Patent: September 23, 2008Assignee: Applera CorporationInventors: Achim E. Karger, Elena V. Bolchakova
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Patent number: 7422872Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.Type: GrantFiled: December 11, 2006Date of Patent: September 9, 2008Assignee: Applied Biosystems Inc.Inventors: James E. Rozzelle, Elena V. Bolchakova
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Patent number: 7148340Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.Type: GrantFiled: November 22, 2002Date of Patent: December 12, 2006Assignee: Applera CorporationInventors: James E. Rozzelle, Elena V. Bolchakova
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Patent number: 7052877Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.Type: GrantFiled: November 22, 2002Date of Patent: May 30, 2006Assignee: Applera CorporationInventors: James E. Rozzelle, Elena V. Bolchakova
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Publication number: 20030211593Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.Type: ApplicationFiled: November 22, 2002Publication date: November 13, 2003Applicant: APPLERA CORPORATIONInventors: James E. Rozzelle, Elena V. Bolchakova
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Publication number: 20030198978Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.Type: ApplicationFiled: November 22, 2002Publication date: October 23, 2003Applicant: APPLERA CORPORATIONInventors: James E. Rozzelle, Elena V. Bolchakova
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Publication number: 20030194726Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase enzyme from a thermophilic organism, Thermus oshimai. The invention also provides methods for using these nucleic acids and polypeptides.Type: ApplicationFiled: November 22, 2002Publication date: October 16, 2003Applicant: APPLERA CORPORATIONInventors: Elena V. Bolchakova, James E. Rozzelle
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Publication number: 20030175756Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus igniterrae. The invention also provides methods for using these nucleic acids and polypeptides.Type: ApplicationFiled: November 22, 2002Publication date: September 18, 2003Applicant: APPLERA CORPORATIONInventors: Elena V. Bolchakova, James E. Rozzelle