Patents by Inventor Elena V. Bolchakova

Elena V. Bolchakova has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20170175093
    Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Application
    Filed: March 6, 2017
    Publication date: June 22, 2017
    Inventors: Elena V. BOLCHAKOVA, James ROZZELLE
  • Publication number: 20160312195
    Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.
    Type: Application
    Filed: July 13, 2016
    Publication date: October 27, 2016
    Inventors: James E. ROZZELLE, Elena V. Bolchakova
  • Patent number: 9382522
    Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus scotoductus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Grant
    Filed: August 19, 2009
    Date of Patent: July 5, 2016
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Elena V. Bolchakova, James E. Rozzelle
  • Patent number: 8318469
    Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Grant
    Filed: December 13, 2005
    Date of Patent: November 27, 2012
    Assignee: Applied Biosystems, LLC
    Inventors: James E. Rozzelle, Elena V. Bolchakova
  • Publication number: 20120082979
    Abstract: Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating misligation products using a particular ligase under reaction conditions and using reagents where that ligase is at least less prone to misligation. In certain embodiments, the recombinant ligase from Archaeoglobus fulgidus (Afu) is employed due to its unique misligation properties.
    Type: Application
    Filed: July 18, 2011
    Publication date: April 5, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Achim E. KARGER, James E. Rozzelle, Chien-Wei Chang, Elena V. Bolchakova
  • Publication number: 20100323406
    Abstract: The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.
    Type: Application
    Filed: August 17, 2009
    Publication date: December 23, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: Paolo Vatta, John W. Brandis, Elena V. Bolchakova, Sandra L. Spurgeon
  • Publication number: 20100086970
    Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus scotoductus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Application
    Filed: August 19, 2009
    Publication date: April 8, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Elena V. BOLCHAKOVA, James E. Rozzelle
  • Publication number: 20090311709
    Abstract: Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating misligation products using a particular ligase under reaction conditions and using reagents where that ligase is at least less prone to misligation. In certain embodiments, the recombinant ligase from Archaeoglobus fulgidus (Afu) is employed due to its unique misligation properties.
    Type: Application
    Filed: July 14, 2009
    Publication date: December 17, 2009
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Achim E. KARGER, James E. Rozzelle, Chien-Wei Chang, Elena V. Bolchakova
  • Publication number: 20090209008
    Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.
    Type: Application
    Filed: August 18, 2008
    Publication date: August 20, 2009
    Applicant: APPLIED BIOSYSTEMS INC.
    Inventors: JAMES E. ROZZELLE, ELENA V. BOLCHAKOVA
  • Publication number: 20090068665
    Abstract: Ligation-based methods and kits are disclosed for identifying at least two target nucleotides in a mixed population sample that is a sample that contains or potentially contains target nucleic acid sequences from more than one source. Typically, two ligation reaction compositions are formed ligation products generated, and the ligation products or their surrogates are analyzed to identify target nucleotides in the mixed population sample. In certain embodiments, the target nucleic acid sequences, the ligation products, or both are amplified. In certain embodiments, multiplex amplification and/or ligation reactions are performed.
    Type: Application
    Filed: July 31, 2008
    Publication date: March 12, 2009
    Applicant: APPLIED BIOSYSTEMS INC.
    Inventors: Achim E. KARGER, Elena V. BOLCHAKOVA
  • Patent number: 7427479
    Abstract: Ligation-based methods and kits are disclosed for identifying at least two target nucleotides in a mixed population sample, that is a sample that contains or potentially contains target nucleic acid sequences from more than one source. Typically, two ligation reaction compositions are formed, ligation products generated, and the ligation products or their surrogates are analyzed to identify target nucleotides in the mixed population sample. In certain embodiments, the target nucleic acid sequences, the ligation products, or both are amplified. In certain embodiments, multiplex amplification and/or ligation reactions are performed.
    Type: Grant
    Filed: April 29, 2005
    Date of Patent: September 23, 2008
    Assignee: Applera Corporation
    Inventors: Achim E. Karger, Elena V. Bolchakova
  • Patent number: 7422872
    Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.
    Type: Grant
    Filed: December 11, 2006
    Date of Patent: September 9, 2008
    Assignee: Applied Biosystems Inc.
    Inventors: James E. Rozzelle, Elena V. Bolchakova
  • Patent number: 7148340
    Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.
    Type: Grant
    Filed: November 22, 2002
    Date of Patent: December 12, 2006
    Assignee: Applera Corporation
    Inventors: James E. Rozzelle, Elena V. Bolchakova
  • Patent number: 7052877
    Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Grant
    Filed: November 22, 2002
    Date of Patent: May 30, 2006
    Assignee: Applera Corporation
    Inventors: James E. Rozzelle, Elena V. Bolchakova
  • Publication number: 20030211593
    Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.
    Type: Application
    Filed: November 22, 2002
    Publication date: November 13, 2003
    Applicant: APPLERA CORPORATION
    Inventors: James E. Rozzelle, Elena V. Bolchakova
  • Publication number: 20030198978
    Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Application
    Filed: November 22, 2002
    Publication date: October 23, 2003
    Applicant: APPLERA CORPORATION
    Inventors: James E. Rozzelle, Elena V. Bolchakova
  • Publication number: 20030194726
    Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase enzyme from a thermophilic organism, Thermus oshimai. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Application
    Filed: November 22, 2002
    Publication date: October 16, 2003
    Applicant: APPLERA CORPORATION
    Inventors: Elena V. Bolchakova, James E. Rozzelle
  • Publication number: 20030175756
    Abstract: The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, Thermus igniterrae. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Application
    Filed: November 22, 2002
    Publication date: September 18, 2003
    Applicant: APPLERA CORPORATION
    Inventors: Elena V. Bolchakova, James E. Rozzelle