Patents by Inventor Emil P. Kartalov

Emil P. Kartalov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210012860
    Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.
    Type: Application
    Filed: July 23, 2020
    Publication date: January 14, 2021
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer
  • Patent number: 10889863
    Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: January 12, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer, Mark D. Goldberg
  • Patent number: 10770170
    Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: September 8, 2020
    Assignee: California Institute of Technology
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer
  • Publication number: 20200216155
    Abstract: Methods to build implantable micro-sensors used to detect tissue-dissolved inert gas and to detect microbubble formation to avoid Caisson disease are described. The disclosed methods and devices are based on measuring physical changes in hydrophobic liquids after absorbing an inert gas such as nitrogen. The teachings of the disclosure help improve safety and efficiency of diving operations.
    Type: Application
    Filed: December 18, 2019
    Publication date: July 9, 2020
    Inventors: Emil P. KARTALOV, Axel SCHERER
  • Patent number: 10457937
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: October 29, 2019
    Assignees: CALIFORNIA INSTITUTE OF TECHNOLOGY, UNIVERSITY OF SOUTHERN CALIFORNIA, CURATE BIOSCIENCES LLC
    Inventors: Emil P. Kartalov, Cheng-Chung Lee, Paul Predki
  • Publication number: 20190127799
    Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.
    Type: Application
    Filed: August 13, 2018
    Publication date: May 2, 2019
    Inventors: Emil P. KARTALOV, Aditya RAJAGOPAL, Axel SCHERER, Mark D. GOLDBERG
  • Publication number: 20190032112
    Abstract: The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.
    Type: Application
    Filed: August 1, 2018
    Publication date: January 31, 2019
    Inventors: Aditya Rajagopal, Emil P. Kartalov
  • Publication number: 20190034583
    Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.
    Type: Application
    Filed: March 7, 2018
    Publication date: January 31, 2019
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer
  • Patent number: 10174313
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: January 8, 2019
    Assignees: CALIFORNIA INSTITUTE OF TECHNOLOGY, UNIVERSITY OF SOUTHERN CALIFORNIA, CURATE BIOSCIENCES LLC
    Inventors: Emil P. Kartalov, Cheng-Chung Lee, Paul Predki
  • Patent number: 10077475
    Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.
    Type: Grant
    Filed: February 26, 2015
    Date of Patent: September 18, 2018
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer, Mark D. Goldberg
  • Patent number: 10066263
    Abstract: The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: September 4, 2018
    Assignee: California Institute of Technology
    Inventors: Aditya Rajagopal, Emil P. Kartalov
  • Publication number: 20180171331
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Application
    Filed: January 31, 2018
    Publication date: June 21, 2018
    Inventors: Emil P. KARTALOV, Cheng-Chung LEE, Paul PREDKI
  • Publication number: 20180171330
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Application
    Filed: January 31, 2018
    Publication date: June 21, 2018
    Inventors: Emil P. KARTALOV, Cheng-Chung LEE, Paul PREDKI
  • Patent number: 9920315
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: March 20, 2018
    Assignees: CALIFORNIA INSTITUTE OF TECHNOLOGY, UNIVERSITY OF SOUTHERN CALIFORNIA, CURATE BIOSCIENCES LLC
    Inventors: Emil P. Kartalov, Cheng-Chung Lee, Paul Predki
  • Publication number: 20170362636
    Abstract: The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.
    Type: Application
    Filed: June 15, 2017
    Publication date: December 21, 2017
    Inventors: Aditya Rajagopal, Emil P. Kartalov
  • Publication number: 20160208242
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Application
    Filed: January 15, 2016
    Publication date: July 21, 2016
    Inventors: Emil P. KARTALOV, Cheng-Chung LEE, Paul PREDKI
  • Publication number: 20150322395
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Application
    Filed: July 17, 2015
    Publication date: November 12, 2015
    Inventors: Emil P. KARTALOV, Darryl SHIBATA, Clive TAYLOR, Lawrence A. WADE
  • Patent number: 9149805
    Abstract: A microfluidic fluid separator for separating target components of a fluid by filtration is described. Methods for separating target components of a fluid by filtration and methods for processing blood on a large scale with the microfluidic fluid separator are provided.
    Type: Grant
    Filed: June 7, 2013
    Date of Patent: October 6, 2015
    Assignees: UNIVERSITY OF SOUTHERN CALIFORNIA, APIC CORPORATION, CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Emil P. Kartalov, Axel Scherer, Koichi Sayano
  • Publication number: 20150167092
    Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.
    Type: Application
    Filed: February 26, 2015
    Publication date: June 18, 2015
    Inventors: Emil P. KARTALOV, Aditya RAJAGOPAL, Axel SCHERER, Mark D. GOLDBERG
  • Patent number: 9037209
    Abstract: An implantable diagnostic device in accordance with the present disclosure includes a probe assembly that can be implemented in a variety of ways. A few example implementations include: a needle inside which is located a bio-sensor chip (the needle being insertable into a human being); a compact package containing the bio-sensor chip (the compact package configured for placement inside a catheter); or a silicon-based bio-sensor package configured for insertion into a vein.
    Type: Grant
    Filed: December 6, 2012
    Date of Patent: May 19, 2015
    Assignees: SANOFI, CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Axel Scherer, Remi Brouard, III, Emil P. Kartalov, Jingqing Huang