Patents by Inventor Erasmus Huber
Erasmus Huber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20040127689Abstract: Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for HIV protease inhibitors. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group common to all HIV protease inhibitors, e.g., saquinavir, nelfinavir, indinavir, amprenavir, ritonavir, lopinavir, and atazanavir.Type: ApplicationFiled: September 24, 2003Publication date: July 1, 2004Inventors: Gerald F. Sigler, Raymond A. Hui, Ina Deras, Richard Terry Root, Mitali Ghoshal, Erasmus Huber, Herbert W. Von Der Eltz, Sigrun Metz, Peter Kern
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Publication number: 20040081957Abstract: A method for quantifying an HIV protease inhibitor in a sample includes combining HIV protease, a conjugate comprising an HIV protease inhibitor analog, and a sample suspected of containing an HIV protease inhibitor. The HIV protease and the conjugate are capable of forming a detectable complex. The method also includes measuring the amount of the detectable complex, and relating the amount of the detectable complex to a concentration of the HIV protease inhibitor in the sample.Type: ApplicationFiled: October 29, 2002Publication date: April 29, 2004Inventors: Herbert von der Eltz, Lili Arabshahi, Haijuan Li, Erasmus Huber
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Patent number: 6524808Abstract: The present invention provides a method for the measurement of an analyte in biological samples whereby an uncompetitive inhibitor is coupled to a ligand and utilized in a homogeneous assay. The analyte can be a drug or drug derivative, hormone, polypeptide, or oligonucleotide. The present invention also provides novel compounds, assay reagents and packaged kits useful for performing such measurements.Type: GrantFiled: June 26, 2000Date of Patent: February 25, 2003Assignee: Roche Diagnostics CorporationInventors: Allan R. Dorn, Salvatore J. Salamone, Mitali Ghoshal, Eva Hoess, Erasmus Huber, Ronald C. Hawley, John W. Patterson
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Publication number: 20020119497Abstract: The present invention relates to methods for detection or measurement of an analyte in a sample by a binding assay comprising at least one hapten/anti-hapten or hapten-like binding pair. More specifically, the invention is directed to processes and test kits comprising the hapten or hapten-like molecule of such binding pair in free form.Type: ApplicationFiled: August 20, 2001Publication date: August 29, 2002Inventors: Thomas Wild, Erasmus Huber, Johann Karl, Peter Sluka, Peter Stegmueller, Norbert Wild
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Patent number: 5976812Abstract: The invention concerns activated amphetamine derivatives, a process for their production as well as their use for producing immunogens or detection conjugates carrying amphetamine groups. A further subject matter of the invention are new conjugates carrying amphetamine groups, a process for the production thereof as well as their use for the production of antibodies or for the determination of amphetamine or derivatives thereof.Type: GrantFiled: July 17, 1997Date of Patent: November 2, 1999Assignee: Roche Diagnostics GmbHInventors: Erasmus Huber, Christian Klein, Rudolf Vogel, Bruno Zink, Wolfgang Rollinger
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Patent number: 5952185Abstract: The invention addresses new photo-activatable biotin derivatives having the formula I ##STR1## wherein R=?(CH.sub.2).sub.n --O.sub.m !.sub.q --?(CH.sub.2).sub.r --O.sub.s !.sub.t --(CH.sub.2).sub.pwith n,r=2,3; m,s=0,1; q+t=1-4; p=1-4,wherein the sum of all CH.sub.2 groups does not exceed 10and wherein R1 as a single or multiple substituent is hydrogen, C.sub.1 -C.sub.5 alkyl, NH.sub.2, COOH, F, Cl, or Br.and their preparation, their use to inactivate streptavidin, the use of the inactivated streptavidin to reduce interference in immunoassays.Type: GrantFiled: October 27, 1997Date of Patent: September 14, 1999Assignee: Boehringer Mannheim GmbHInventors: Erasmus Huber, Michael Wiedmann, Frederic Donie
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Patent number: 5821065Abstract: A method for determining collagen degradation products in a body fluid sample using a non-competitive or competitive immunoassay is disclosed, the method comprising incubating the body fluid sample with an amount of antibody which specifically reacts with the amino acid sequence of SEQ ID NO. 2 of collagen l or a fragment thereof, and determining the collagen degradation products bound to the amount of the antibody. A kit for such a method is also disclosed.Type: GrantFiled: December 21, 1995Date of Patent: October 13, 1998Assignee: Boehringer Mannheim GmbHInventors: Werner Naser, Brigitte Drager, Ulrich Essig, Christa Hubner-Parajsz, Erasmus Huber
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Patent number: 5763272Abstract: Disclosed are antibodies which specifically bind the amino acid sequence of SEQ ID NO. 1 of collagen I. A hybridoma cell line which produces the antibodies is also disclosed.Type: GrantFiled: July 9, 1996Date of Patent: June 9, 1998Assignee: Boehringer Mannheim GmbHInventors: Werner Naser, Brigitte Drager, Ulrich Essig, Christa Hubner-Parajsz, Erasmus Huber
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Patent number: 5662911Abstract: Benzodiazepine protein conjugates of the formula I ##STR1## wherein R1 is hydrogen, a methyl group or R, R2 is hydrogen, a hydroxyl or an OR group, with R1 or R2 containing an R group,R3 is a halogen, NO2 or NH2,X is hydrogen or a halogen,wherein R is a group of the formula II, wherein Z is a macromolecular immunogen reactive carrier substance and n is 2 or 3.The benzodiazepine protein conjugates are used as immunogens to obtain antibodies to benzodiazepines.The invention also relates to an immunoassay for the detection of benzodiazepines where the so generated antibodies are used.Type: GrantFiled: January 24, 1996Date of Patent: September 2, 1997Assignee: Boehringer Mannheim GmbHInventors: Erasmus Huber, Christian Klein, Hans-Peter Josel, Bruno Zink
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Patent number: 5601824Abstract: Compound of the general formula ##STR1## wherein R is an ester-activating group, m and n are identical or different and 0-6, p =0, 2-4, and q and s=1, p=0 if s=0 and p=2-4 if s=1. The compound is novel and suitable for the reproducible coupling of immunological substances at a high yield.Type: GrantFiled: February 23, 1996Date of Patent: February 11, 1997Assignee: Boehringer Mannheim GmbHInventors: Eva Hoess, Erasmus Huber, Christine Markert-Hahn, Beatus Ofenloch-Haehnle
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Patent number: 5573922Abstract: An immunological method for the determination of triazine and triazine derivatives by a competitive immunoassay in which the sample and labelled atrazine compete for a polyclonal antibody which is bound to a solid phase before, during or after the immunological reaction, the immunological complex binds to the solid phase, the solid phase and unbound labelled atrazine are separated and the label in the solid or liquid phase is determined as a measure of the content of triazine and triazine derivatives, which is characterized in that polyclonal antibodies which were obtained by immunization with a 4-alkylamino-S-triazine are used and a conjugate of a label and 4-amino-S-triazine which is bound to the label via the 6 position is used as the labelled atrazine. Triazine and triazine derivatives can be determined simultaneously using this method.Type: GrantFiled: April 29, 1994Date of Patent: November 12, 1996Assignee: Boehringer Mannheim GmbHInventors: Eva Hoess, Erasmus Huber, Christine Markert-Hahn, Wolfgang Rollinger
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Patent number: 5525474Abstract: Novel derivatives of procainamide and N-acetylprocainamide (NAPA) are disclosed having the following formula: ##STR1## wherein: X=hydrogen or acetyl;n=1 to p where p=MW of Z/1000;Z=a poly(amino acid) or polysaccharide; andR3=a bond or ##STR2## wherein R2=an alkyl, cycloalkyl or aryl group having 2 to 10 carbon atoms. The derivatives include maleimide conjugates of proteins or poly(amino acids), enzymes, enzyme donor polypeptides and labeling substances. Novel activated hapten intermediates useful in the preparation of the conjugates and methods for synthesis of the hapten intermediates and derivatives are also disclosed.Type: GrantFiled: January 31, 1994Date of Patent: June 11, 1996Assignee: Boehringer Mannheim CorporationInventors: Gerald F. Sigler, Charles F. Walter, Todd Glancy, Erasmus Huber, Frank E. Klein
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Patent number: 5521319Abstract: The invention concerns a compound of the general formula (I): ##STR1## in which Bi denotes a residue derived by cleavage of a carboxyl group from biotin or from a biotin derivative, R.sup.1 and R.sup.2 denote independently of one another hydrogen or C.sub.1 --C.sub.4 alkyl,n denotes an integer from 4 to 10 andX denotes an alkylene residue with a chain length of 5 to 20 atoms substituted by one or several O or/and S atoms and a conjugate of this compound with a substance that has at least one primary or/and secondary amino group NHR.sup.3.Type: GrantFiled: September 14, 1994Date of Patent: May 28, 1996Inventors: Erasmus Huber, Bruno Zink, Helmut Lenz, Eva Hoess
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Patent number: 5519142Abstract: Compound of the general formula ##STR1## wherein R is an ester-activating group, m and n are identical or different and 0-6, p=0, 2-4, and q and s=1, p=0 if s=0 and p=2-4 if s=1. The compound is novel and suitable for the reproducible coupling of immunological substances at a high yield.Type: GrantFiled: March 29, 1994Date of Patent: May 21, 1996Assignee: Boehringer Mannheim GmbHInventors: Eva Hoess, Erasmus Huber, Christine Markert-Hahn, Beatus Ofenloch-Haehnle
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Patent number: 5514559Abstract: Novel conjugate of the general formulaA--L.sub.1 --L.sub.2 --Bwherein A and B are the substances to be coupled selected from the groups of haptens, proteins, polysaccharides, soluble polystyrenes, or peptides, L.sub.1 and L.sub.2 are trivalent linkers and L.sub.1 and L.sub.2 are bound via two thioether. They can be used in immunological assays and exhibit an improved storage stability.Type: GrantFiled: March 29, 1994Date of Patent: May 7, 1996Assignee: Boehringer Mannheim GmbHInventors: Christine Markert-Hahn, Beatus Ofenloch-Haehnle, Eva Hoess, Erasmus Huber
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Patent number: 5219764Abstract: Hapten-biotin conjugates in which the hapten is linked with biotin via a spacer, which has 26 to 40 atoms in its chain and contains at least 5 heteroatoms, are novel and are suitable, in particular for use in a competitive homogeneous immunoassay in which the agglutination which occurs in the reaction is evaluated by turbidimetric or nephelometric measurements.Type: GrantFiled: April 10, 1991Date of Patent: June 15, 1993Assignee: Boehringer Mannheim GmbHInventors: Erasmus Huber, Dietmar Zdunek, Christian Klein, Roland Schenk
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Patent number: 5198537Abstract: The present invention provides digoxigenin derivatives of the formula: ##STR1## wherein n is a whole number of from 1 to 4 and Z is a carboxyl group or a functional derivative derived therefrom. The present invention also provides digoxigenin conjugates of the general formula: ##STR2## wherein the carrier is an immunogenic carrier material, a nucleic acid or a labelled immunocomponent of a labelled digoxigenin immunoconjugate for use in an immunoassay, m is a number from 1 to the number of coupling positions available on the carrier and Y is the group formed by the reaction of the carboxyl function Z of the digoxigenin derivative of general formula (I) with the reacting position of the carrier material. Also described are uses of these conjugates as nucleic acid probes, immunogens, and in immunoassay.Type: GrantFiled: October 25, 1989Date of Patent: March 30, 1993Assignee: Boehringer Mannheim GmbHInventors: Erasmus Huber, Klaus Muhlegger, Herbert von der Eltz, Bruno Zink
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Patent number: 5171679Abstract: The present invention provides cobalamin acid hydrazides of the general formula:B--CO--NH--NH--R--CO--NH--NH).sub.x H (I)wherein B is a residue formed from a cobalamin by the splitting off of a --CONH.sub.2 group, R is a spacer and x is 0 or 1, as well as a process for the preparation thereof.The present invention also provides cobalamin conjugates of the general formula:B--d--CO--NH--NH--R--CO--NH).sub.x N.dbd.GP (II)in which B, R and x have the above-given meanings and d signifies the position of --CO--NH--, as well as a process for the preparation thereof. These cobalamin conjugates are very suitable for use in immunoassays, especially for the determination of cyanocobalamin.Type: GrantFiled: January 5, 1990Date of Patent: December 15, 1992Assignee: Boehringer Mannheim GmbHInventors: Erasmus Huber, Josef Dieckhoff, Christian Klein, Konrad Kurzinger
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Patent number: 5032518Abstract: The present invention provides a hapten-protein conjugate, wherein a hapten is bound to the reducing end of a sugar which consists of up to 10 monosaccharide units and on a free CH.sub.2 OH group on the other end of the sugar, which is in the .alpha.-position to a hydroxyl group, is bound a protein.Type: GrantFiled: January 25, 1989Date of Patent: July 16, 1991Assignee: Boehringer Mannheim GmbHInventors: Erasmus Huber, Hans-Georg Batz, Herbert von der Eltz, Christian Klein
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Patent number: 4990443Abstract: The present invention provides hapten derivatives of the general formula: ##STR1## wherein Hap is a residue formed from a hapten carrying a keto or aldehyde group by splitting off an oxo group and R.sup.1 and R.sup.2, which can be the same or different, are alkyl radicals containing up to 7 carbon atoms and one of the symbols R.sup.1 and R.sup.2 can also represent a hydrogen atom.The present invention also provides hapten-protein conjugates of the general formula: ##STR2## wherein Hap, R.sup.1 and R.sup.2 have the above-given meanings and E--NH is the residue of a protein bound via an .epsilon.-amino group of a lysine residue.Type: GrantFiled: January 24, 1989Date of Patent: February 5, 1991Assignee: Boehringer Mannheim GmbHInventors: Erasmus Huber, Christian Klein, Gunter Pappert, Klaus Hallermayer