Patents by Inventor Eugeny A. Lukhtanov

Eugeny A. Lukhtanov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11155713
    Abstract: The carborhodamine dyes disclosed herein are novel reagents suitable for automated incorporation of carborhodamine dyes into oligonucleotides that can be used in detection methods for nucleic acid targets. This disclosure provides an efficient and simple process for the preparation of carborhodamine compounds and introduces previously unknown reagents for the automated synthesis of oligonucleotide-carborhodamine conjugates.
    Type: Grant
    Filed: May 6, 2019
    Date of Patent: October 26, 2021
    Assignee: ELITechGroup, Inc.
    Inventor: Eugeny A. Lukhtanov
  • Patent number: 10975423
    Abstract: Methods, primers and probes are provided for the isothermal amplification and detection, without denaturation, of double stranded nucleic acid targets for polymerase strand displacement amplification (“iSDA”). The methods and compositions disclosed are highly specific for nucleic acid targets with high sensitivity, specificity and speed that allow detection of clinical relevant target levels. The methods and compositions can easily be used to amplify or detect nucleic acid targets in biological samples.
    Type: Grant
    Filed: October 23, 2019
    Date of Patent: April 13, 2021
    Assignee: ELITECHGROUP, INC.
    Inventors: Yevgeniy S. Belousov, Eugeny A. Lukhtanov, Noah Scarr
  • Publication number: 20210041360
    Abstract: Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Application
    Filed: October 9, 2020
    Publication date: February 11, 2021
    Applicant: ELITechGroup, Inc.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Patent number: 10890529
    Abstract: Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Grant
    Filed: February 12, 2020
    Date of Patent: January 12, 2021
    Assignee: ELITECHGROUP, INC.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Publication number: 20200299755
    Abstract: Fluorescence quenching nitrodiarylethene analogs are useful in oligonucleotide conjugates and probes. These analogs, whose absorption spectra are substantially blue-shifted relatively to emission spectra of common fluorophores (such as fluorescein), do not need to rely on spectral overlap of quencher absorbance and fluorophore's emission for their quenching abilities. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Application
    Filed: April 9, 2020
    Publication date: September 24, 2020
    Applicant: ELITechGroup, Inc.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Patent number: 10738346
    Abstract: Fluorescence quenching nitrodiarylethene analogs are useful in oligonucleotide conjugates and probes. These analogs, whose absorption spectra are substantially blue-shifted relatively to emission spectra of common fluorophores (such as fluorescein), do not need to rely on spectral overlap of quencher absorbance and fluorophore's emission for their quenching abilities. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Grant
    Filed: March 1, 2018
    Date of Patent: August 11, 2020
    Assignee: ELITECHGROUP, INC.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Patent number: 10677728
    Abstract: Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Grant
    Filed: August 7, 2018
    Date of Patent: June 9, 2020
    Assignee: ELITECHGROUP B.V.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Publication number: 20200173924
    Abstract: Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Application
    Filed: February 12, 2020
    Publication date: June 4, 2020
    Applicant: ELITechGroup, Inc.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Publication number: 20200040387
    Abstract: Methods, primers and probes are provided for the isothermal amplification and detection, without denaturation, of double stranded nucleic acid targets for polymerase strand displacement amplification (“iSDA”). The methods and compositions disclosed are highly specific for nucleic acid targets with high sensitivity, specificity and speed that allow detection of clinical relevant target levels. The methods and compositions can easily be used to amplify or detect nucleic acid targets in biological samples.
    Type: Application
    Filed: October 23, 2019
    Publication date: February 6, 2020
    Applicant: ELITechGroup, Inc.
    Inventors: Yevgeniy S. Belousov, Eugeny A. Lukhtanov, Noah Scarr
  • Publication number: 20190367735
    Abstract: The carborhodamine dyes disclosed herein are novel reagents suitable for automated incorporation of carborhodamine dyes into oligonucleotides that can be used in detection methods for nucleic acid targets. This disclosure provides an efficient and simple process for the preparation of carborhodamine compounds and introduces previously unknown reagents for the automated synthesis of oligonucleotide-carborhodamine conjugates.
    Type: Application
    Filed: May 6, 2019
    Publication date: December 5, 2019
    Applicant: ELITechGroup, Inc.
    Inventor: Eugeny A. Lukhtanov
  • Publication number: 20190064067
    Abstract: Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Application
    Filed: August 7, 2018
    Publication date: February 28, 2019
    Applicant: ElitechGroup B.V.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Patent number: 10127349
    Abstract: The invention provides methods to identify pRNA- and pDNA oligomer affinity pairs. Affinity pairs comprised of nucleic acid oligomers which demonstrate no cross-reactivity (“orthogonal”) are designed using software and empirically verified by thermodynamic study and lateral flow testing. The design software uses a semi-random algorithm to build such sequences of nucleic acid oligomers based on user-input parameters for affinity strength and orthogonal stringency. These pairs can be applied for use in multi-analyte solid support and lateral flow diagnostic tests.
    Type: Grant
    Filed: July 18, 2011
    Date of Patent: November 13, 2018
    Assignee: ELITECHGROUP B.V.
    Inventors: Noah Scarr, Eugeny A. Lukhtanov
  • Publication number: 20180258471
    Abstract: Fluorescence quenching nitrodiarylethene analogs are useful in oligonucleotide conjugates and probes. These analogs, whose absorption spectra are substantially blue-shifted relatively to emission spectra of common fluorophores (such as fluorescein), do not need to rely on spectral overlap of quencher absorbance and fluorophore's emission for their quenching abilities. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets.
    Type: Application
    Filed: March 1, 2018
    Publication date: September 13, 2018
    Applicant: ElitechGroup B.V.
    Inventors: Eugeny A. Lukhtanov, Noah Scarr
  • Patent number: 9169256
    Abstract: Artificial nucleosides including 2-methyl-nucleobase-substituted butane-1,3-diol nucleosides are disclosed. Four different stereoisomers of such nucleosides are possible. Oligonucleotides made up of the artificial nucleosides form homoduplexes of greater stability than DNA duplexes and have a reduced ability to hybridize to DNA or RNA.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: October 27, 2015
    Assignee: ELITechGroup B.V.
    Inventors: Noah Scarr, Eugeny A. Lukhtanov
  • Patent number: 9085800
    Abstract: 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
    Type: Grant
    Filed: March 23, 2012
    Date of Patent: July 21, 2015
    Assignee: Elitech Holding B.V.
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov, Noah Scarr
  • Patent number: 9056887
    Abstract: Minor groove binder phosphoramidites having the formula M-L-PA, wherein M is a minor groove binder comprising a protected heteroaromatic amine, L is a linker, and PA is a phosphoramidite group, have been synthesized. Preferred methods of synthesis include synthesizing a minor groove binder intermediate containing a transiently protected hydroxyl group, protecting heteroaromatic amines of the corresponding minor groove binder intermediate as carbamate intermediates, reacting the carbamate intermediate to remove the transient protecting group to yield carbamate-protected minor groove binder agent as an intermediate with a free hydroxyl group, and converting the intermediate with a free hydroxyl group to the desired minor groove binder phosphoramidite. These minor groove binder phosphoramidites are useful in the preparation of oligonucleotide conjugates, particularly those for use as probes and primers.
    Type: Grant
    Filed: July 24, 2012
    Date of Patent: June 16, 2015
    Assignee: Elitech Holding B.V.
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov
  • Patent number: 8969003
    Abstract: 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogs can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogs can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: March 3, 2015
    Assignee: Elitech Holding B.V.
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov, Noah Scarr
  • Publication number: 20140275508
    Abstract: Artificial nucleosides including 2-methyl-nucleobase-substituted butane-1,3-diol nucleosides are disclosed. Four different stereoisomers of such nucleosides are possible. Oligonucleotides made up of the artificial nucleosides form homoduplexes of greater stability than DNA duplexes and have a reduced ability to hybridize to DNA or RNA.
    Type: Application
    Filed: March 13, 2014
    Publication date: September 18, 2014
    Applicant: Elitech Holding B.V.
    Inventors: Noah Scarr, Eugeny A. Lukhtanov
  • Patent number: 8569516
    Abstract: Compounds useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: February 17, 2006
    Date of Patent: October 29, 2013
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • Publication number: 20130261014
    Abstract: 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
    Type: Application
    Filed: March 14, 2013
    Publication date: October 3, 2013
    Inventors: Alexei Vorobiev, Eugeny A. Lukhtanov, Noah Scarr