Abstract: A process for preparation of RNA using an affinity column and a DNAzyme is disclosed. By the process, a target RNA can be mass-produced with a high yield, a high resolution and a high purification efficiency. The process includes the steps of preparing a RNA adduct including a target RNA and a tail RNA sequence which is affixed to the 3′-terminal of the target RNA and can complementary combine with an oligo-dN sequence of an oligo-dN affinity column; purifying the RNA adduct by combining the RNA adduct to the oligo-dN affinity column; and obtaining the target RNA by cleaving the purified RNA adduct with a DNAzyme. Preferably, the process for preparation of RNA further includes the steps of removing a RNA fragment cleaved by the DNAzyme by using an affinity column; and decomposing the DNAzyme with a DNA nuclease, and then removing decomposed DNAzyme fragments with a gel filtration column.