Abstract: Embodiments are directed to sharing namespaces across file system clusters. A file in a spoke file system may be determined based on a command provided to the spoke file system such that the file may be associated with an inode mask. Data blocks associated with the file may be determined based on the command and the inode mask such that the data blocks may be absent from the spoke file system. Requests for leases may be generated based on the absent data blocks such that each lease request corresponds to a portion of the absent data blocks Employing the leases provided by a hub file system to: copy the absent data blocks from the hub file system where each portion of absent data blocks may be associated with a lease; updating the inode mask to include the leases and each copied portion of absent blocks.

Abstract: A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.

Abstract: A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.

Abstract: Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.